What are Silencer Select siRNAs?
Silencer Select siRNAs are the next generation Ambion siRNAs. These siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNAs with unrivalled efficacy, potency, and specificity. In siRNA screening applications, the result is fewer false negatives due to poor silencing and fewer false positives due to off-target effects. Silencer Select siRNAs yield cleaner, more consistent data from primary screen, reducing time and resources to find true, validated targets. As a result, there are fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data.
Ambion Silencer Select siRNAs are available in 96- or 384-well plates format plated to your specifications. These siRNA libraries enable medium and high-throughput analysis of phenotypes resulting from inhibition of sets of targets from specific functional classes or your favorite list of targets.
- Superior efficacy—Reduce off target effects by up to 90% due to latest design algorithms and LNA chemical modifications
- Highest efficiency—Perform knockdown studies with 100-fold more potent siRNAs than currently available siRNAs
- Dependable—Achieve faster discoveries via consistency of gene knockdown and reliability of phenotypic results
- Convenient—Reduce data de-convolution and accelerate target validation with our 96- and 384-well arrayed format
- Customizable—Choose the best option for your workflow demands, including additional siRNA scales and pooled formats
Pre-defined Silencer Select siRNA Libraries
The Silencer Select Human Epigenetics siRNA Library is a comprehensive collection of highly potent, chemically modified siRNAs targeting enzyme classes characterized in epigenetic pathways. These include protein families mediating epigenetic signaling through the acetylation and methylation of histones. The Silencer Select Human Epigenetics siRNA Library is an excellent solution for investigating the impact of loss of gene function on developmental, disease, or evolutionary biology. The Silencer Select Human Epigenetics siRNA Library contains three siRNAs per target for 451 epigenetic enzymes, ensuring comprehensive coverage of epigenetics-based gene function studies. This siRNA library is supplied as 0.25 nmol per well in 96-well plates.
The Silencer Select Human Transcription Factor siRNA Library targets genes expressing proteins that bind DNA in a sequence-specific manner to control the rate of transcription. The Silencer Human Transcription Factor siRNA Library contains three siRNAs per target for 2,167 known transcription factors. This siRNA library is supplied as 0.25 nmol per well in 96-well plates.
The Silencer Select Human DNA Damage Response siRNA Library targets genes expressing surveillance proteins that monitor DNA integrity and that can activate cell cycle checkpoints and DNA repair pathways in response to DNA damage to prevent potentially deleterious mutations. The Silencer Select Human DNA Damage Response siRNA Library allows potent and highly specific screening using three siRNAs per target for 582 DNA damage gene response targets, providing an essential and focused platform to investigate cellular pathways that sense, signal, and repair DNA lesions following DNA damage. The superior LNA chemistry–modified siRNA design ensures accurate RNAi screening. This siRNA library is supplied as 0.25 nmol per well in 96-well plates.
The Silencer Select Human Drug Targets siRNA Library includes genes involved with apoptosis, senescence, nucleic acid binding, autophagy, DNA repair, and characterized nuclear receptors, along with other genes that are considered potential targets for therapeutics. This library includes siRNAs targeting genes with gene IDs from the Thermo Fisher Scientific Druggable Genome and Druggable Genome Extension that are not included in the kinase, phosphatase, protease, ion channel, GPCR, or ubiquitin siRNA Libraries. The Silencer Select Human Drug Targets siRNA Library contains three unique, non-overlapping siRNAs per target for 7,926 genes. This siRNA library is supplied as 0.25 nmol per well in 96- and 384-well plates.
The Silencer Select Human Apoptosis siRNA Library provides investigators with a focused siRNA screening platform targeting the most up-to-date apoptosis gene targets, thus enabling a detailed analysis of the apoptotic signaling pathway. The Silencer Select Human Apoptosis siRNA Library contains three unique, non-overlapping siRNAs per target for 924 genes. The Silencer Select siRNA design algorithm combined with LNA chemistry allows potent and highly specific RNAi screening, offering confidence in your apoptosis investigations. This siRNA library is supplied as 0.25 nmol per well in 96-well plates.
The Silencer Select Human Drug Transporter siRNA Library contains siRNAs targeting 99 transporter genes that play key roles in pharmacology, affecting entry and extrusion of drugs into and out of cells. Targets also include those important in the absorption, distribution, metabolism, and excretion (ADME) of many drugs. The Silencer Select Human Drug Transporter siRNA Library offers a highly effective approach to RNAi screens, assessing genes important for drug compounds’ bioavailability and efficacy. This siRNA library is supplied as 0.25 nmol per well in 384-well plates.
The Silencer Select Human Cell Cycle Regulation siRNA Library V4 includes siRNAs targeting 1,471 genes whose functions are essential for controlling cell growth and division. The gene targets include cyclin-dependent kinases (CDKs) involved in cell cycle progression, members of the CIP/KIP and INK4 family of cell cycle inhibitors, members of the retinoblastoma protein family, and factors important for DNA replication including cell division cycle proteins (CDCs). Each gene is targeted by three unique, non-overlapping siRNAs that are derived using industry-leading design algorithms. Additional LNA chemical modifications are employed to enhance efficacy, specificity, and potency, resulting in more accurate and consistent silencing data when studying genes critical to cell cycle regulation. This siRNA library is supplied as 0.25 nmol per well in 96-well plates.
The Silencer Select Human Membrane Trafficking siRNA Library V4 is designed for researchers targeting proteins known or predicted to participate in membrane trafficking or remodeling, with an aim to understand gene expression in critical cellular functions including biosynthesis, secretion, endocytosis, lysosomal and proteasomal protein degradation, and phagocytosis. The Silencer Select Human Membrane Trafficking siRNA Library targets 142 genes with three unique, non-overlapping siRNAs each. Each siRNA is identified using industry-leading design algorithms, and LNA chemical modifications are applied for improved efficacy, specificity, and potency, enabling accurate and consistent silencing data for genes involved in cell membrane trafficking. This siRNA library is supplied as 0.25 nmol per well in 96-well plates.
Ubiquitination is an enzymatic-based, post-translational modification process essential to a wide array of cellular processes. These include immunology, apoptosis, biogenesis, DNA transcription and repair, cell cycle and division, cell stress, modulation of cell receptors and neural or muscular degeneration as well as disease and diagnostic associations. The Silencer Select Human Ubiquitin siRNA Library targets 981 genes, offering a comprehensive set of targets for RNAi-based analysis of the ubiquitin pathway. The siRNAs are identified using industry-leading design algorithms and include LNA chemical modifications to ensure efficacy, specificity, and potency, minimizing false positives and offering accurate and consistent silencing data for the study of the ubiquitin signaling pathway. This siRNA library is supplied as 0.25 nmol per well in 96-well plates.
The Silencer Select Human Cancer Genome siRNA Library contains gene targets commonly affected in cancers. Often, changes in these genes affect pathways in cells, causing them to divide and survive when they normally would die. The Silencer Select Human Cancer Genome siRNA Library contains three unique, non-overlapping siRNAs targeting each of 532 cancer-associated genes. The Silencer Select siRNAs are designed with industry-leading design algorithms and include LNA modifications to enable efficacy, specificity, and potency, offering accurate and consistent silencing data for RNAi screens in cancer target discovery. This siRNA library is supplied as 0.25 nmol per well in 96-well plates.
Cell surface receptors (membrane receptors, transmembrane receptors) are integral membrane proteins that take part in communication between cells and their surroundings. Extracellular signaling molecules (usually hormones, neurotransmitters, cytokines, growth factors or cell recognition molecules) attach to the receptor, triggering changes in the function of the cell. For this process called signal transduction, we have created the Silencer Select Human Cell Surface siRNA Library, which contains three unique, non-overlapping siRNAs for each of 794 cell surface receptor genes. Industry-leading siRNA design algorithms and the application of LNA modifications help ensure efficacy, specificity, and potency, offering accurate and consistent silencing data in screens of cell surface–based signaling factors. This siRNA library is supplied as 0.25 nmol per well in 96-well plates.
The Silencer Select Human Tumor Suppressor siRNA Library contains siRNAs targeting 745 tumor suppressor genes, or anti-oncogenes, which protect cells on the path to cancer. Mutations that cause loss or reduction in function can result in the progression to cancer, usually in combination with other genetic changes. The Silencer Select Human Tumor Suppressor siRNA Library allows highly robust RNAi screening using three unique, non-overlapping siRNAs per gene target. Silencer Select Human Tumor Suppressor siRNA designs are based on industry-leading design algorithms and include LNA chemical modifications to enable efficacy, specificity, and potency. Thus, this library offers an essential platform for accurate and consistent silencing data for studying the role of tumor suppressor genes. This siRNA library is supplied as 0.25 nmol per well in 96-well plates.
Three individual siRNAs per target provided in a convenient 96-well format
Increasingly, researchers are performing genome-scale surveys of gene function. The Silencer Select Human Genome siRNA Library is ideal for this purpose. Three unique, non-overlapping siRNAs are provided for each of 21,585 human targets. The siRNAs targeting the “druggable” portion of this library are arranged by gene functional class to enable easy screening of important gene subsets such as kinases, phosphatases, GPCRs, etc. This siRNA library is supplied in 384-well plates.
With siRNAs targeting the most therapeutically relevant human genes and conveniently grouped by target gene class, the Silencer Select Human Druggable Genome siRNA Library and Silencer Select Human Extended Druggable Genome siRNA Library are popular choices for many researchers. The druggable genome set targets 9,032 genes, whereas the extended druggable genome collection targets those genes plus the "Extension Set," for a total of 10,415 genes, including transcription factors. Please contact us for the complete list of targets. These libraries are available in both 96- and 384-well plate formats.
Because of their importance in cell signaling and many biological pathways, kinases are key drug targets and subjects of intense scrutiny. The Silencer Select Human Kinase siRNA Library targets 710 human kinases with three individual Silencer Select siRNAs per gene. This siRNA library enables systematic yet cost- effective RNAi studies of these key cell regulators. Validated siRNAs are included where available. The Silencer Select Human Kinase siRNA Library is provided in 96-well plates.
Along with kinases, phosphatases are important regulators of biological pathways and cell signaling cascades. The Silencer Select Human Phosphatase siRNA Library targets 298 human phosphatases with three individual siRNAs per gene supplied in 96-well plates. This library is the ideal companion to the Silencer Select Kinase siRNA Library , enabling more detailed studies of biological pathways.
G-protein–coupled receptors, or GPCRs, are key components of many signal transduction pathways and also represent an important class of druggable genes. The Silencer Select Human GPCR siRNA Library targets 380 non-olfactory human GPCRs with three individual Silencer Select siRNAs per target supplied in 96-well plates.
Focused siRNA sets against human proteases, ion channels, and nuclear hormone receptors provide a cost- effective means to study these important regulators of cellular function. These siRNA libraries are each supplied in 96-well plates. The Silencer Select Human Protease siRNA Library features three unique, non-overlapping siRNAs per target for 494 human proteases. The Silencer Select Human Nuclear Hormone Receptor siRNA Library includes three siRNAs per target for 47 nuclear hormone receptors. The Silencer Select Human Ion Channel siRNA Library features three siRNAs per target for 338 human ion channels.
Custom Silencer Select siRNA libraries
The ultimate in flexibility
Do you have a favorite list of genes you would like to target with a collection of siRNAs? We can prepare a custom Silencer Select siRNA library for any set of human genes. The minimum order is only 36 siRNAs. All you need to supply is a list of genes or transcripts identified by NCBI Entrez Gene ID or RefSeq mRNA accession number. With this user-friendly option, you can specify the order of the siRNAs on the plate, as well as the amount of siRNA per order (1 nmol minimum), number of aliquots on each plate, and the number of siRNAs per target. siRNAs can be provided individually or pooled.
Individual siRNAs for enhanced data reliability
Silencer Select siRNA Libraries feature three individual siRNAs for each target. Screening with three siRNAs per gene significantly decreases both false positive and false negative rates, resulting in enhanced confidence in RNAi screening data, reduced chance of missing important genes, and less time spent following up on false positive hits from the screen.
These libraries contain 0.25 nmol of each siRNA, sufficient for 500 transfections (at 5 nM siRNA, 100 µL transfection volume). The siRNAs are supplied dried into the wells of 96 well plates. One column of each 96 well plate is left empty to allow for the addition of controls at the time of use. The libraries are delivered with full siRNA sequence information, as well as the exon targeted and each targeted gene's symbol, full name, aliases, RefSeq number, and Entrez Gene ID, plus results from any validation experiments performed using the included siRNAs.
Silencer Select siRNA Libraries to these or any other list of human genes can also be provided with 1, 2, or 5 nmol of each siRNA. Custom formatting is available with these larger sizes. If you would like a gene list or other information, or would like to discuss your research with one of our technical experts, contact your local sales representative or e-mail us at RNAisupport@thermofisher.com
* Guarantee for Silencer Select Pre-designed siRNAs
We guarantee that when you purchase two Silencer Select Pre-designed siRNAs to the same target, then those two siRNAs will silence the target mRNA by 70% or more. To qualify for the guarantee, the siRNAs must have been transfected at ≥5 nM and mRNA levels detected 48 hours post-transfection. Real-time RT-PCR is recommended but not required for this application. Customers must also show sufficient knockdown with a positive control siRNA to demonstrate transfection efficiency. If the guaranteed level of knockdown is not observed and an appropriate positive control is successful, a new Silencer Select siRNA sequence will be synthesized free of charge. This guarantee does not extend to any replacement product.