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The SOLiD® Fragment Library Construction Kit provides a fully optimized set of reagents for constructing 10 fragment libraries for use with the SOLiD® Next Generation Sequencing System.

Reach your sequencing goals with confidence

The kit is available in 3 configurations (Table 1). The SOLiD® Fragment Library Construction Kit with SizeSelect™ Gels contains reagents, columns, and gels for use with the SOLiD® standard protocol. The SOLiD® Fragment Library Construction Kit for use with the express protocol includes all reagents and columns. The SOLiD® Fragment Library Construction Reagents Kit contains no purification components.

The SOLiD® Fragment Library Construction Kit:

  • Enables flexibility—choice of 3 kit configurations for use with standard or express SOLiD® protocols
  • The optimized and recommended protocol for use with the SOLiD® System
  • Components quality-tested together to enable optimal results
Enlarge Image
Figure 1: Click to enlarge.

How SOLiD® Fragment Library Construction works

Fragment libraries consist of short lengths of DNA specifically selected for optimal performance with a next generation sequencing platform. Briefly, genomic DNA is sheared, primer adaptors are ligated to the fragmented DNA followed by an end-polishing step, and the library is amplified using primers specific to the adaptors (Figure 1). The fragments are size-selected, purified, and are then ready for the sequencing reactions.

The SOLiD® Fragment Library Construction Kit is specifically optimized for use with the SOLiD® Sequencing System.

Table 1

Product Part Number SOLiD® Fragment Library Construction Reagents PureLink™ PCR Purification Kit (A11199) E-Gel® SizeSelect 2% 10 Pack
SOLiD® Fragment Library Construction Kit
with SizeSelect™ Gels
4443471YesYesYes
SOLiD® Fragment Library Construction Kit4443473YesYesNo
SOLiD® Fragment Library Construction Reagents4443713YesNoNo
Note: The SOLiD® Fragment Library Oligo Kit (Cat. No. 4401151) is not included and must be purchased separately. For research use only. Not intended for animal or human therapeutic or diagnostic use, unless otherwise indicated.

SOLiD® Software For Your Application

SOLiD BioScope
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