Mate-Paired Library Construction Kits for SOLiD® Next-Generation Sequencing
Structural variations are present throughout the genome and are associated with various diseases. Mate-paired libraries, in conjunction with high-throughput SOLiD® next generation sequencing, provide a valuable method for detecting genomic rearrangements such as insertions, deletions, duplications, and translocations.
The SOLiD® Mate-Paired Library Construction Kits provide a fully optimized set of reagents for constructing mate-paired libraries for sequencing using the SOLiD® System.
Mate-paired libraries are produced by randomly shearing DNA, size-selecting fragments, and bringing the fragment ends into close proximity by recircularization upon a synthetic oligonucleotide adaptor. Further manipulations produce a library of fragments that allows the sequence of both ends of each fragment to be determined sequentially. Aligned to a reference genome, the orientation and distance between the mate-pair sequences provide critical information for genomic assembly and identification of genomic variants.
Two mate-paired library construction methods are available. The SOLiD® Long Mate-Paired Library Construction Kit uses a protocol that captures ~50 bp on either end of the circularized fragment and uses a time-course S1 Nuclease digestion of the DNA (Figure 1). The SOLiD® 2 x 25 bp Mate-Paired Library Construction Kit uses a protocol that captures ~25 bp of either end of the circularized fragment and uses a restriction digestion with EcoP15 to generate the fragments (Figure 2). In general, capture of longer ends is preferred to provide as much information as possible and facilitate bioinformatic mapping. However, for several reasons including biases of each method, consistency of cuts, prior research, and organisms of interest, it may be desirable to generate libraries using both protocols.
The SOLiD® Mate-Paired Library Construction Kits:
- Enable a convenient and simplified workflow — includes all reagents, columns, and gels for the construction of ten libraries (see note)
- Optimized and recommended protocols for use with the SOLiD® System
- Components quality-tested together to help ensure optimal results
|Product||Part Number||SOLiD® 2x25 Mate-Paired Enzyme Kit||SOLiD® Long Mate Paired Enzyme Kits 1 & 2||SOLiD® Mate-Paired Bead & Buffer Kit||SOLiD® Library Column Purification Kit||SOLiD® Library Quick Gel Extraction Kit|
|SOLiD® 2x25 bp Mate-Paired Library Construction Kit||4443472||Yes||No||Yes||Yes||Yes|
|SOLiD® Long Mate Paired Library Construction Kit||4443474||No||Yes||Yes||Yes||Yes|
|SOLiD® Long Mate Paired Library Construction Reagents and Gel Extraction Modules||4443711||No||Yes||Yes||No||Yes|
|Note: Does not include components of the SOLiD® Mate-Paired Library Oligos Kit (Cat #4400468), EcoP15I (used in the 2 x 25 bp protocol), or S-adenosylmethionine (used in the 2 x 25 bp protocol), which must be purchased separately. For research use only. Not intended for animal or human therapeutic or diagnostic use, unless otherwise indicated.|
SOLiD® Software For Your Application
- SOLiD® BioScope™ Software