Diverse applications for gold-standard sequencing technology
Sanger sequencing is the gold standard for sequencing technology: it provides a high degree of accuracy, long-read capabilities, and the flexibility to support a diverse range of applications in many research areas. Sanger sequencing is highly recognized for DNA sequencing applications, but also supports applications in RNA sequencing and epigenetic analysis.
Applications and methods
Sanger sequencing can be used to determine the accuracy of CRISPER- and TALEN-mediated genome editing techniques in complex organisms.
Sanger sequencing can be used as an orthogonal method to confirm variants identified by next-generation sequencing (NGS).
The Applied Biosystems HIV-1 Genotyping Kit harnesses gold-standard Sanger sequencing technology to enable reliable genotyping of the genetically diverse HIV-1 virus from plasma and dried blood spot (DBS) samples to detect resistance to protease inhibitors, nucleoside reverse-transcriptase inhibitors, and non-nucleoside reverse-transcriptase inhibitors.
Ion AmpliSeq technology is ideal for routine labs working with a limited number of samples/targets, as well as high-throughput labs that need an orthogonal method for confirming minor alleles.
Molecular profiling of cancers is becoming more and more important in personalized cancer therapy research, or precision oncology. Sanger sequencing is ideal for low-level variant detection in material containing minimal amounts of DNA, such as formalin-fixed, paraffin-embedded (FFPE) tissues.
Mitochondrial DNA sequencing is a useful tool for researchers studying human diseases such as diabetes, certain cancers, and mechanisms of aging. It is also used in population genetics and biodiversity assessments and is important for human identification and forensics applications.
Human leukocyte antigen (HLA) typing is used to match patients and donors for bone marrow or cord blood transplants.
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Featured application notes
- Using Sanger sequencing to facilitate CRISPR and TALEN-mediated genome editing workflows
- Detect low-level somatic mutations in FFPE samples using an extended RAS research assay
- A simplified DNA extraction method for Sanger sequencing of FFPE samples
- Sanger sequencing using Ion Ampliseq Primers and Libraries
- Microbial identification by Sanger sequencing of the 16S rRNA gene using MicrobeBridge software
For Research Use Only. Not for use in diagnostic procedures.