PCR Cloning Support
Explore our “Getting Started” and “Troubleshooting” sections for solutions to top inquiries and common problems. Browse through our "Guides and Tools" section to access a comprehensive portfolio of product-related support resources.
- What are some of the prerequisites for TOPO® cloning?
- What is the best ratio of insert:vector to use? Is there an equation to calculate this?
- What are the advantages of using a TOPO® TA cloning system compared to traditional TA cloning?
- Do I need to gel-purify my PCR product for TOPO® cloning?
- What can inhibit the TOPO® cloning reaction?
- What are the possible causes of sequencing problems with the TOPO® TA vectors, and are there any suggestions to help solve these issues?
- I’m getting no colonies after transformation.
- I’m able to get a lot of colonies, however, none contain my insert of interest.
- I’m seeing a lot of vector only colonies when I try to perform a negative control reaction using vector only (no insert) for a TOPO® reaction. Is my TOPO® vector re-ligating?
- What happens when a vector adapted with the topoisomerase enzyme is stored at -80 degrees C?
Guides and Tools
- Overview: Introduction to Cloning
- Overview: Directional TOPO® Cloning
- Overview: The Technology Behind TOPO® Cloning
- Overview: Molecular Cloning (legacy Thermo Scientific™)
- Overview: Cloning Workflow (legacy Thermo Scientific™)
- Overview: 5 Cloning Shortcuts (legacy Thermo Scientific™)
- Overview: Molecular Cloning Infographic (legacy Thermo Scientific™)
- Overview: Checking Your T4 DNA Ligase Activity (legacy Thermo Scientific™)
- Overview: 5 Ways to Screen for Recombinant Clones (legacy Thermo Scientific™)
- General: Molecular Cloning Resource Library (legacy Thermo Scientific™)
- Guide: PCR & Molecular Cloning Handbook
- Protocol: Cloning of Taq Polymerase-amplified PCR Products
- Protocol: Cloning of A-tailed PCR Fragments Using Conventional Ligase Method
- Selection Table: TOPO® Cloning Kits By Application
- Video: CloneJET: An Advanced Positive Selection System for High-efficiency Cloning of PCR Products (legacy Thermo Scientific™)
If you have a favorite cloning vector or a vector that you wish to use for a specific assay, let our TOPO® Cloning experts adapt it for you. Any vector you choose can be TOPO® adapted for high efficiency cloning of PCR products in 5 minutes.
Gene synthesis outperforms traditional cloning by providing fast turnaround times and guaranteed sequence accuracy. Construct design and optimization services are also available in order to fine tune the expression of your gene(s) of interest.
GeneArt® Plasmid Services include flexible offerings covering construction of plasmid vectors customized for your needs, and subcloning of your sequence into any vector, including the Gateway® vector system. Concentrate on your research goals and leave your plasmid construction and subcloning work to us.
For Research Use Only. Not for use in diagnostic procedures.