The TOPO™ Cloning Advantage

Thermo Fisher Scientific's TOPO cloning technology simplifies and improves PCR cloning by replacing ligase and long ligation times with a cloning vector that has been “activated” with topoisomerase I. This activated vector supports room-temperature ligation in 5 minutes and yields a very high percentage of recombinants. Simply put, no other cloning technology is as fast or as efficient.

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For more information or to order a service, call 800.955.6288 x2, or email custom.services@thermofisher.com

How It Works

Practically any vector you choose can be adapted for TOPO cloning of PCR products at high efficiencies. Our scientists will design the cloning strategy and review it with you, modify your vector by covalently attaching the topoisomerase I enzyme, and prepare the vector ends for either blunt or TA Cloning vectors. If you choose, we can also prepare your vector for directional cloning of PCR products. Each vector is functionally tested in TOPO cloning reactions to ensure guaranteed cloning efficiencies.

High-Throughput Compatible
Functionally tested TOPO-adapted custom vectors are supplied in bulk, for a minimum of 500 reactions, so they are HTP compatible. In addition, you'll receive high-efficiency competent cells in bulk or in a 96-well format for HTP cloning. You won't have to gather additional reagents to complete your cloning projects—so you'll save even more time.

Activate YOUR Vector!

Thermo Fisher Scientific supplies numerous TOPO vectors for many common cloning needs. We can also TOPO-adapt any custom vector. If you are identifying, cloning, and expressing hundreds or thousands of PCR-amplified genes in your high-throughput (HTP) experiments, save many hours of time by allowing us to adapt your preferred vector with TOPO technology. The advantages of our service are:

  • No need to switch protocols and reagents to a standard TOPO vector—you can continue to use the vector that best suits your cloning needs, yet still enjoy the advantage of 5-minute ligations at room temperature
  • Greatly increased cloning efficiency over non-adapted vectors
  • No need to change any downstream assays or experimental parameters