Custom Goat Polyclonal Antibody Production Protocols
Immunization and serum-collection procedures for custom goat polyclonal antibody production
Our comprehensive array of Thermo Scientific Pierce custom antibody services includes a customizable option for goat immunization, serum-collection and titer analysis for goat antibody production, plus several extension possibilities.
Highlights
- Custom goat antibodies - a reliable protocol for immunizing goats, collecting serum, testing bleeds, reporting titer results and delivering antisera
- Track and customize project - easily add additional goats to the default protocol; track and manage projects online with OpenProject Tool: you decide which animals to continue or terminate (see Extensions below)
- Full service integration - combine this immunization service with our complete set of services for antigen preparation and antibody purification (see Custom Polyclonal Antibodies pages for details)
This goat immunization schedule uses two mixed-breed goats (usually Boer/Nubian or Alpine, unless specifically requested) for each MAP-peptide, conjugated-peptide, or protein immunogen. Injections are intradermal (ID) as emulsions in Complete Freund's Adjuvant (CFA) or Incomplete Freund's Adjuvant (IFA); alternative adjuvants can be used if requested.
120-day 2-goat immunization protocol for goat polyclonal antibody production
Antigen preparation, such as peptide synthesis and/or immunogen conjugation, occurs before Day 0. Injection amounts are given for a conjugated peptide antigen (e.g., KLH), MAP-peptide, or protein immunogen. Protocol Days are approximate (± 2 days).
Procedure | Protocol day | Description |
---|---|---|
Control serum collection | Day 0 | Pre-immune bleed (~5 mL per goat) |
Primary injection | Day 1 | Immunize with 0.50 mg antigen in CFA, ID 20 sites |
1st booster | Day 14 | Boost with 0.50 mg antigen in IFA, ID 20 sites |
2nd booster | Day 28 | Boost with 0.50 mg antigen in IFA, ID 20 sites |
Serum collection | Day 42 | Test-bleed (~5 mL) per goat |
3rd booster | Day 56 | Boost with 0.50 mg antigen in IFA, ID 20 sites |
Serum collection | Day 56 | Test-bleed (~5mL) per goat |
4th booster | Day 70 | Boost with 0.50 mg antigen in IFA, ID 20 sites |
5th booster | Day 84 | Boost with 0.50 mg antigen in IFA, ID 20 sites |
Serum collection | Day 84 | Test-bleed (~5 mL) per goat |
6th booster | Day 98 | Boost with 0.50 mg antigen in IFA, ID 20 sites |
7th booster | Day 112 | Boost with 0.50 mg antigen in IFA, ID 20 sites |
Serum collection | Day 112 | Test-bleed (~5mL) per goat |
ELISA and shipping | Day 118 | ELISA titration (results available online); Verify disposition of goats; decide to continue or terminate; Plasmapheresis from one goat (~1100 mL serum), taken when requested by customer |
Instruction due date | Day 120 | If no animal instructions are received, per diem charges will apply; Extension protocol is recommended with titer monitoring by ELISA |
Deliverables: 22 vials crude antibody sera
|
Several pre-defined (yet customizable) options are available for extending or continuing the goat immunization and serum-collection schedule. The standard production bleed yields 250mL of goat serum per animal; plasmapheresis yields ~1100mL of serum per goat. The purchase decision to continue antibody production can be made at any time before scheduled termination.
One-month extension protocol for goat antibody production
Procedure | Protocol day | Description |
---|---|---|
1st booster | Day 1 | Boost with 0.50 mg antigen in IFA, 20 ID sites |
Serum collection | Day 14 | Test-bleed (5 mL per goat) |
Deliverables (per goat): 2 vials crude antibody sera
|
One-month extension with production bleed protocol for goat antibody production
Procedure | Protocol day | Description |
---|---|---|
1st booster | Day 1 | Boost with 0.50 mg antigen in IFA, 20 ID sites |
Serum collection | Day 14 | Bleed (250 mL per goat) |
Deliverables (per goat): 2 vials crude antibody sera
|
One-month extension with plasmapheresis protocol for goat antibody production
Procedure | Protocol day | Description |
---|---|---|
1st booster | Day 1 | Boost with 0.50 mg antigen in IFA, 20 ID sites |
Serum collection | Day 14 | Plasmapheresis (1100 mL per goat) |
Deliverables (per goat): 2 vials crude antibody sera
|
Aggressive, one-month extension protocol for goat antibody production
Procedure | Protocol day | Description |
---|---|---|
1st booster | Day 1 | Boost with 0.50 mg antigen in IFA, 4 SQ sites |
2nd booster | Day 7 | Boost with 0.50 mg antigen in IFA, 4 SQ sites |
Serum collection | Day 21 | Test-bleed (~5 mL per goat) |
Deliverables (per goat): 2 vials crude antibody sera
|
Tools and information
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