Whether you're new to real-time PCR, also called quantitative PCR (qPCR), or want to learn about new applications for real-time PCR, we have the learning material, including videos and webinars, to help you understand the technology and get started quickly.

Real-time PCR combines PCR amplification and detection into a single step. This eliminates the need to detect products using gel electrophoresis, and more importantly it enables the method to be truly quantitative. With real-time PCR, fluorescent dyes are used to label PCR products during thermal cycling. Real-time PCR instruments measure the accumulation of fluorescent signal during the exponential phase of the reaction for fast, precise quantification of PCR products and objective data analysis.

Featured resources
Handbook
Optimized protocol with low sample input for profiling human microRNA

Real-Time PCR Handbook

An excellent resource for anyone who is new to real-time PCR and interested in learning the basic principles of the technology.  This 3rd edition contains 6 chapters in 70 pages and also covers topics such as assay design, data analysis, real-time PCR tips, and troubleshooting.

Videos
Ask TaqMan videos

Ask TaqMan videos

Get answers to popular qPCR questions with our library of quick and easy how-to videos covering a variety of topics including experiment setup, best practices, and guidance on products and technologies.

Article

Calculating Primer and Probe Concentrations

Follow example calculations to prepare and use oligonucleotide working stocks with confidence and precision.

Article

Common TaqMan Myths Busted

How much do you know about TaqMan Assays?  Discover some of the common misconceptions researchers have about this chemistry.