Every minute counts when it comes to expressing drug candidates. That’s why the Gibco Expi293 Expression System is the tried-and-true choice for transient expression in 293 cells. This rapid, high-yield system allows you to access recombinant 293–derived proteins in just five to seven days, so you get what you need faster.
“I can produce more protein in less volume and less time, that’s fantastic! It’s like enjoying the benefits of a stable cell line system, without going through the hassle of establishing one. Now we scale up and we can get what we need. It’s robust, it’s scalable, and it’s reproducible. It’s great.” Joe Sheffer, Senior Research Associate at AnaptysBio, Inc.
The Expi293 Expression System brings together a high-expressing 293 cell line, a chemically defined serum–free culture medium, and a high-efficiency transfection reagent with specialized enhancers.
The Expi293 Expression System is designed to deliver up to 6x more protein production in just one week, compared with other transient 293 systems that can take two weeks or more. And it requires less plasticware, which means less waste and more incubator space.
“In all my years working with transient expression systems the Expi293 Expression System is the first one to achieve 2.3 grams per liter, beating every other HEK293 transient expression system.” Large pharmaceutical company
Figure 1. Expression of human IgG and Fc-tagged Cripto achieve expression levels of over 1 g/L in the Expi293 Expression System
For researchers studying G protein–coupled receptors (GPCRs) and other membrane proteins, the Expi293 MembranePro Expression System is designed to deliver enriched, functional membrane proteins with high efficiency, scalability, and reliability.
The Expi293 Expression System is based on expression in mammalian 293 cells, which offers the benefits of native folding and posttranslational modifications. Figure 2 shows purification of the HDAC3/SMRT-DAD complex from 293 cells and E. coli. The 293 cells yield a stable pure HDAC3 complex (Figure 2A), whereas expression in E. coli (Figure 2B) does not.
The Expi293 Expression System is designed to deliver up to 6x more protein production in just one week, compared with other transient 293 systems that can take two weeks or more. And it requires less plasticware, which means less waste and more incubator space.
“In all my years working with transient expression systems the Expi293 Expression System is the first one to achieve 2.3 grams per liter, beating every other HEK293 transient expression system.” Large pharmaceutical company
Figure 1. Expression of human IgG and Fc-tagged Cripto achieve expression levels of over 1 g/L in the Expi293 Expression System
For researchers studying G protein–coupled receptors (GPCRs) and other membrane proteins, the Expi293 MembranePro Expression System is designed to deliver enriched, functional membrane proteins with high efficiency, scalability, and reliability.
The Expi293 Expression System is based on expression in mammalian 293 cells, which offers the benefits of native folding and posttranslational modifications. Figure 2 shows purification of the HDAC3/SMRT-DAD complex from 293 cells and E. coli. The 293 cells yield a stable pure HDAC3 complex (Figure 2A), whereas expression in E. coli (Figure 2B) does not.
The Expi293 Expression System is designed to support robust protein production in a variety of culture vessels. In small and large-scale formats—and everything in between—you can express the protein you need.
Figure 3. Expression of Fc-tagged Cripto achieves similar volumetric protein yields at 3 different scales.
NOTE: Cost comparison based on list price in USD and protein yields of 1 g/L with Expi293 system.
| PEI w/ FreeStyle 293 Medium | Expi293 System (typically delivers 5x yield) |
|
|---|---|---|
| Cost of 1 L transfection | $548 | $1,531 |
| Culture volume for equivalent yield | 1 L | 200 mL |
| Total cost for same amount of protein | $607 | $324 |
Sterile, single-use Thermo Scientific Nalgene flasks are made from light, break-resistant, crystal-clear PETG, so you can express with confidence. All sizes are available in vented or non-vented formats, and with plain or baffled bottoms for use on the benchtop or shaker table. See protocol for flasks validated with Expi293 Expression system.
For Research Use Only. Not for use in diagnostic procedures.
