实时荧光定量PCR拷贝数变异 (CNV) 分析
|TaqMan Copy Number Assays combine TaqMan MGB probe chemistry with Applied Biosystems real-time PCR instruments to provide a method for obtaining specific, reproducible, and easy-to-interpret copy number results. This fast and simple method can be completed in hours rather than days. Use TaqMan Copy Number Assays to screen specific targets with a workflow that can be automated to process several hundred to thousands of samples in a single day. And because TaqMan Assays are the gold standard for accurate target quantitation, they are ideal for use in microarray follow-up studies.|
- Predesigned TaqMan Copy Number Assays
- Human: over 1.6 million predesigned assays available for genome-wide coverage
- Mouse: over 180,000 predesigned assays available for gene exons
- Common vector marker and reporter genes assays
- Custom Plus TaqMan Copy Number Assays
- Submit user-defined human or mouse genomic targets for custom assay design including upfront bioinformatic sequence analysis and in silico QC
- Custom TaqMan Copy Number Assays for other targets of interest
- Submit any masked target sequences for assay design
- Submit primer/probe pair sequences for assay synthesis
- Does not include upfront bioinformatic sequence analysis or in silico QC
Human and mouse TaqMan Copy Number Reference Assays are also available.
As the simplest method available to study copy number variation, the TaqMan Copy Number Assays feature easy assay ordering and a simple workflow to enable quick results.
Experimental Workflow: Find the Products You Need for Every Step
- Determining the Copy Number of Genes Using Real-Time Quantitative PCR
- Selecting SNPs for Genetic Association Studies Based on the Genome-wide Patterns of Linkage Disequilibrium Inferred from the HapMap Project Data
Background References (Web)
- Copy Number Variants and Common Disorders: Filling the Gaps and Exploring Complexity in Genome-Wide Association Studies PLoS Genet 2007; 3(10): e190. doi:10.1371/journal.pgen.0030190 Authors: Estivill X., et. al.
- Detection of large-scale variation in the human genome Nature Genetics 2004; 36, 949 - 951 Authors: A John Iafrate, et. al.
- Large-Scale Copy Number Polymorphism in the Human Genome Science 23 July 2004: Vol. 305. no. 5683, pp. 525 - 528 Authors: Jonathan Sebat, et. al.
- Global variation in copy number in the human genome Nature 444, 444-454 (23 November 2006) Authors: Richard Redon, et. al.
- Differential quantification of CYP2D6 gene copy number by four quantitative real-time PCR assays Pharmacogenetics and Genomics 2010, 20:451-454. Authors: Anuradha Ramamoorthy, et al.
- Recurrent microdeletions at 15q11.2 and 16p13.11 predispose to idiopathic generalized epilepsies Brain. 2010; 133:23-32; doi:10.1093/brain/awp262 Authors: De Kovel, C. G., et. al.
- Copy Number Variants of GSTM1 and GSTT1 in relation to lung cancer risk in a prospective cohort study. Ann Epidemiol. 2009; 19(8):546-52 Authors: Lam, T.K., et. al.
- Sample degradation leads to false-positive copy number variation calls in multiplex real-time polymerase chain reaction assays Analytical Biochemistry. 2009 Mar 15;386(2):288-90. Authors: Cukier H.N., et. al.
- Origins and functional impact of copy number variation in the human genome Nature advance online publication 7 October 2009 | doi:10.1038/nature08516; Received 14 August 2009; Accepted 21 September 2009; Published online 7 October 2009 Authors: Conrad D.F., et. al.
- A recurrent 15q13.3 microdeletion syndrome associated with mental retardation and seizures Nature Genetics. 2008 Mar;40(3):322-8. Authors: Sharp A.J., et. al.
- Recurrent rearrangements of chromosome 1q21.1 and variable pediatric phenotypes The New England Journal of Medicine. 2008 Oct 16;359(16):1685-99. Authors: Mefford H.C., et. al.