Ultimate flexibility and throughput
The Applied Biosystems ProFlex PCR System combines the reliability and performance you’ve come to expect from Applied Biosystems thermal cyclers with the flexible configuration and control features that fit how you work today. Interchangeable block formats allow you to maximize your throughput or run independent experiments concurrently. And now you can access your ProFlex PCR System with Thermo Fisher Connect, giving you the freedom to design and securely upload your methods and monitor runs from any smartphone or desktop computer.
ProFlex PCR System, the thermal cycler with ultimate flexibility and throughput
ProFlex PCR System fits how you work today and tomorrow with 5 interchangeable block formats - maximize your throughput or run three experiments at once.
Quickly, easily swap out blocks to meet your PCR needs
The ProFlex PCR System has 5 different blocks that can be changed with the flip of a switch, including the first-of-its-kind 3 x 32-well block. This block allows up to three experiments to be run simultaneously, completely independently of each other and without interference. Dual 96-well and dual 384-well blocks are available for your high-throughput sequencing needs, and a dual flat block is available to support Applied Biosystems OpenArray plate technology for genotyping analysis on the Applied Biosystems QuantStudio 12K Flex Real-Time PCR System.
Boost productivity with secure remote access to your ProFlex thermal cycler
The ProFlex thermal cycler is a cloud-enabled instrument, allowing you secure private access with a Thermo Fisher Connect account. Anywhere, anytime, with any mobile device or desktop computer you can:
- Design and share protocols
- Schedule an instrument
- Start or stop a run
- Check run status
Setup is easy, and your account comes with 10 GB of free storage.
Store
Design protocols from anywhere; easily maintain run reports.
Develop protocols on any desktop computer or mobile device and store them in your secure personal cloud account. Once your run is prepared, start it from your instrument or remotely with Thermo Fisher Connect. After each run, the run report will be conveniently saved in your account.
Monitor
Check run status and instrument availability in real-time.
Download our Instrument Connect remote monitoring app to:
- Check instrument availability
- Schedule an instrument
- Check run status
Share
Discretionary access to project folders and protocols.
Share your protocols or other files with other Thermo Fisher Connect users on campus and around the world. It’s easy, convenient, and protected by the highest levels of privacy standards.
Learn more about Thermo Fisher Connect ›
For peace of mind, the Thermo Fisher Cloud has high industry-standard security, so your work remains secure. We have partnered with Amazon Web Services™ (AWS) to offer a robust platform.
Precise PCR optimization with VeriFlex technology
VeriFlex Blocks provide a better-than-gradient approach to PCR optimization. With three separate Peltier blocks, you have precise control over three temperature zones.
- Enables highly accurate determination of optimal annealing temperature
- Helps eliminate guesswork often associated with standard gradient cyclers
- Helps save time by running up to three reactions with different annealing temperatures in the same PCR run
- Use your thermal cycler as an incubator; take advantage of precision temperature control
Manuals and specifications
- ProFlex PCR System specification sheet
- ProFlex PCR System quick reference guide
- ProFlex PCR System user guide
- Remote access quick reference guide
- Starter kit user guide (3 x 32-well block)
- Starter kit user guide (384-well block)
- Starter kit user guide (96-well block)
- Starter kit user guide (dual 96-well block)
Application notes
- Thermal cyclers: key thermal cycling concepts and ramp rates
- Reliability of Applied Biosystems thermal cyclers
- Thermal performance of Applied Biosystems thermal cyclers
- VeriFlex temperature control technology for thermal cycling
- Thermal cycler comparison: the ProFlex 96-Well PCR System demonstrates consistent, reliable performance
- Thermal cycler amplification robustness: a comparison of several models
- Sample evaporation: a comparison of several thermal cycler models
- Thermal cycler sample amplification uniformity: a comparison of several models
Stay in control with Thermal Cycler Fleet Control Software
Manage multiple users, methods, and instruments with a single intuitive interface securely behind your firewall
High-performance PCR plastics for optimal PCR results
Applied Biosystems PCR plastics have been designed and validated to work with our thermal cyclers for more than 25 years. Options for every format and throughput need are available to ensure consistent, precise results for any experiment.
ProFlex PCR System, the thermal cycler with ultimate flexibility and throughput
ProFlex PCR System fits how you work today and tomorrow with 5 interchangeable block formats - maximize your throughput or run three experiments at once.
Quickly, easily swap out blocks to meet your PCR needs
The ProFlex PCR System has 5 different blocks that can be changed with the flip of a switch, including the first-of-its-kind 3 x 32-well block. This block allows up to three experiments to be run simultaneously, completely independently of each other and without interference. Dual 96-well and dual 384-well blocks are available for your high-throughput sequencing needs, and a dual flat block is available to support Applied Biosystems OpenArray plate technology for genotyping analysis on the Applied Biosystems QuantStudio 12K Flex Real-Time PCR System.
Boost productivity with secure remote access to your ProFlex thermal cycler
The ProFlex thermal cycler is a cloud-enabled instrument, allowing you secure private access with a Thermo Fisher Connect account. Anywhere, anytime, with any mobile device or desktop computer you can:
- Design and share protocols
- Schedule an instrument
- Start or stop a run
- Check run status
Setup is easy, and your account comes with 10 GB of free storage.
Store
Design protocols from anywhere; easily maintain run reports.
Develop protocols on any desktop computer or mobile device and store them in your secure personal cloud account. Once your run is prepared, start it from your instrument or remotely with Thermo Fisher Connect. After each run, the run report will be conveniently saved in your account.
Monitor
Check run status and instrument availability in real-time.
Download our Instrument Connect remote monitoring app to:
- Check instrument availability
- Schedule an instrument
- Check run status
Share
Discretionary access to project folders and protocols.
Share your protocols or other files with other Thermo Fisher Connect users on campus and around the world. It’s easy, convenient, and protected by the highest levels of privacy standards.
Learn more about Thermo Fisher Connect ›
For peace of mind, the Thermo Fisher Cloud has high industry-standard security, so your work remains secure. We have partnered with Amazon Web Services™ (AWS) to offer a robust platform.
Precise PCR optimization with VeriFlex technology
VeriFlex Blocks provide a better-than-gradient approach to PCR optimization. With three separate Peltier blocks, you have precise control over three temperature zones.
- Enables highly accurate determination of optimal annealing temperature
- Helps eliminate guesswork often associated with standard gradient cyclers
- Helps save time by running up to three reactions with different annealing temperatures in the same PCR run
- Use your thermal cycler as an incubator; take advantage of precision temperature control
Manuals and specifications
- ProFlex PCR System specification sheet
- ProFlex PCR System quick reference guide
- ProFlex PCR System user guide
- Remote access quick reference guide
- Starter kit user guide (3 x 32-well block)
- Starter kit user guide (384-well block)
- Starter kit user guide (96-well block)
- Starter kit user guide (dual 96-well block)
Application notes
- Thermal cyclers: key thermal cycling concepts and ramp rates
- Reliability of Applied Biosystems thermal cyclers
- Thermal performance of Applied Biosystems thermal cyclers
- VeriFlex temperature control technology for thermal cycling
- Thermal cycler comparison: the ProFlex 96-Well PCR System demonstrates consistent, reliable performance
- Thermal cycler amplification robustness: a comparison of several models
- Sample evaporation: a comparison of several thermal cycler models
- Thermal cycler sample amplification uniformity: a comparison of several models
Stay in control with Thermal Cycler Fleet Control Software
Manage multiple users, methods, and instruments with a single intuitive interface securely behind your firewall
High-performance PCR plastics for optimal PCR results
Applied Biosystems PCR plastics have been designed and validated to work with our thermal cyclers for more than 25 years. Options for every format and throughput need are available to ensure consistent, precise results for any experiment.
Resources
Plastics compatibility chart for Applied Biosystems instruments
PCR and cDNA Synthesis Support Center
Tips and troubleshooting help
School of Molecular Biology, PCR Education
Technical content for new and experienced molecular biologists
Thermal Cycler Software and Firmware Files
Download the latest updates for your instrument
Interested in private label thermal cyclers?
Submit an inquiry
Service
We provide a full range of service options for all Applied Biosystems thermal cyclers.
Service plans
Support
Additional services available
Instrument qualification service: Have one of our engineers perform temperature qualifications at your site. You may also contact Instrument Services to purchase a temperature verification tool and perform the qualification yourself.
For Research Use Only. Not for use in diagnostic procedures.