TOPO TA Cloning with Mach1 Competent Cells

TOPO cloning kits with Mach1-T1R competent cells make cloning fast and simple. Mach1 cells deliver robust growth for miniprep analysis in just four hours, saving up to 2 days compared to restriction enzyme and ligation cloning.

We offer a suite of TOPO cloning kits with Mach1-T1^R competent cells for basic cloning of Taq-amplified fragments, blunt-ended fragments, sequencing, long-fragment PCR cloning, and entry into the Gateway system.  Simply add your PCR product (blunt-ended fragment or amplified using Taq polymerase) to the appropriate vector, incubate for 5 minutes and transform into our fast growing Mach1 competent cells.

Mach1 competent cells are also available with Zero Blunt and TOPO XL vectors:

• Zero Blunt TOPO PCR Cloning Kit for Sub-cloning with Mach1 Competent Cells
• TOPO XL PCR Cloning Kit with One Shot Mach1 Competent Cells
• Zero Blunt TOPO PCR Cloning Kit for Sequencing with One Shot Mach1 Competent Cells