Protect your precious samples

The Attune NxT is designed for improved sample processing and on-time project runs, and greater instrument endurance. Expand flow cytometry use to include previously troublesome samples including larger cells and dissociated tissue masses.

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The technology behind less clogging includes:

  • Syringe-based system for controlled sample volume and pressure entry at 75 PSI (compared to a max pressure of 15 PSI for traditional flow cytometers)
  • Larger flow cell: 200 µm in diameter
  •  Rapid acquisition speeds even with diluted samples, reducing the likelihood of cell-cell interactions that can clog the system

Invitrogen Attune NxT Flow Cytometer Demo Video
Designed for faster experimental run times, the Invitrogen Attune NxT compact flow cytometer offers rapid and accurate analysis for a broad range of sample types. Experience the difference.

Is Clogging Getting in the Way of Pizza?!
Scientists racing to get pizza before it is done are clogging at doors. Clogging is not a problem with the Invitrogen Attune NxT Flow Cytometer as it is with other cytometers that leads to sample loss, project delays, and the need to monitor.

Clogging is good in Holland, not in Labs. The Attune NxT Flow Cytometer
Researchers frustrated by clog dancing in the lab. Clogging is great in Holland, not in the lab. The Attune NxT Flow Cytometer.

Flow Cytometry - Attune NxT Non-clogging flow cytometer - Rube Goldberg
Traditionally, flow cytometers clog with large clumpy samples. This leads to sample loss, project delays, and the need to monitor. Invitrogen Attune NxT flow cytometer is designed to reduce the risk of clogging and protecting your precious samples.

Principles behind clog resistant technology

Volumetric sample entry works like a pipette. Tension is created with a plunger pushing down (Figure 1A). The tension is released with the plunger coming up with the sample (Figure 1B). Clogging is less likely to occur under this high and controlled pressure environment.

volumetric flow cytometry sample entry

Figure 1. The syringe-based system for sample introduction is one of anti-clogging design features of the Attune NxT Flow Cytometer.

Educational resources

The Attune NxT Flow Cytometer is designed to minimize the hassle, frustration and sample loss associated with a clogged instrument and bring high-throughput statistical analysis to an increasing number of sample types. Investigate difficult samples and large, clumpy cells including cancer cells, cardiomyocytes and adipocytes, previously not amenable to flow cytometry.

See examples of different cell types analyzed on the Attune NxT Flow Cytometer below (Table 1).

Application note: Transcription factor expression analysis of hPSC-derived cardiomyocytes


Table 1. Cell types successfully analyzed on the Attune NxT Flow Cytometer

Sample type Demonstrated example Published References
Algae Nannochloropsis oculata  
Bacteria Escherichia coli
Bacillus subtilis
Legionella strains
Staphyloccus aureus  
Diatom Phaeodactylum tricornutum
Human Human adenocarcinoma
Human bone marrow (PBMCs)
Human bone osteosarcoma cells (U2OS)
Human cardiomyocytes  
Human corneal epithelial cells  
Human embryonic kidney (293FT)
Human endothelial cells
Human leukocytes  
Human mesenchymal stem cell (hMSC)
Human red blood cells (RBC)  
Human T cell leukemia (Jurkat)
Human whole blood
Mouse Mouse splenocytes
Mouse T and B regulatory cells
Mouse cardiomyocytes
Mouse bone marrow
Mouse lung tissue
Mouse tumor cells
Mouse skeletal muscle
Parasites Plasmodium falciparum
L. amazonensis, T. brucei and T. cruzi
Trypanosoma cruzi
Picophytoplankton Prochlorococcus spp;
Synechococcus spp
 
Plants Arabidopsis thaliana  
Phalaris arundinacea
Solidago canadensis
Yeast Candida albicans
Saccharomyces cerevisiae
Zebra fish Zebra fish organs

Customer stories

See how Dr. Bruno Sainz from Madrid Autónoma University describes his no-clog experience.  Learn the benefits of Attune technology to analyze tumor masses.

Invitrogen Attune NxT Flow Cytometer Demo Video
Designed for faster experimental run times, the Invitrogen Attune NxT compact flow cytometer offers rapid and accurate analysis for a broad range of sample types. Experience the difference.

Is Clogging Getting in the Way of Pizza?!
Scientists racing to get pizza before it is done are clogging at doors. Clogging is not a problem with the Invitrogen Attune NxT Flow Cytometer as it is with other cytometers that leads to sample loss, project delays, and the need to monitor.

Clogging is good in Holland, not in Labs. The Attune NxT Flow Cytometer
Researchers frustrated by clog dancing in the lab. Clogging is great in Holland, not in the lab. The Attune NxT Flow Cytometer.

Flow Cytometry - Attune NxT Non-clogging flow cytometer - Rube Goldberg
Traditionally, flow cytometers clog with large clumpy samples. This leads to sample loss, project delays, and the need to monitor. Invitrogen Attune NxT flow cytometer is designed to reduce the risk of clogging and protecting your precious samples.

Principles behind clog resistant technology

Volumetric sample entry works like a pipette. Tension is created with a plunger pushing down (Figure 1A). The tension is released with the plunger coming up with the sample (Figure 1B). Clogging is less likely to occur under this high and controlled pressure environment.

volumetric flow cytometry sample entry

Figure 1. The syringe-based system for sample introduction is one of anti-clogging design features of the Attune NxT Flow Cytometer.

Educational resources

The Attune NxT Flow Cytometer is designed to minimize the hassle, frustration and sample loss associated with a clogged instrument and bring high-throughput statistical analysis to an increasing number of sample types. Investigate difficult samples and large, clumpy cells including cancer cells, cardiomyocytes and adipocytes, previously not amenable to flow cytometry.

See examples of different cell types analyzed on the Attune NxT Flow Cytometer below (Table 1).

Application note: Transcription factor expression analysis of hPSC-derived cardiomyocytes


Table 1. Cell types successfully analyzed on the Attune NxT Flow Cytometer

Sample type Demonstrated example Published References
Algae Nannochloropsis oculata  
Bacteria Escherichia coli
Bacillus subtilis
Legionella strains
Staphyloccus aureus  
Diatom Phaeodactylum tricornutum
Human Human adenocarcinoma
Human bone marrow (PBMCs)
Human bone osteosarcoma cells (U2OS)
Human cardiomyocytes  
Human corneal epithelial cells  
Human embryonic kidney (293FT)
Human endothelial cells
Human leukocytes  
Human mesenchymal stem cell (hMSC)
Human red blood cells (RBC)  
Human T cell leukemia (Jurkat)
Human whole blood
Mouse Mouse splenocytes
Mouse T and B regulatory cells
Mouse cardiomyocytes
Mouse bone marrow
Mouse lung tissue
Mouse tumor cells
Mouse skeletal muscle
Parasites Plasmodium falciparum
L. amazonensis, T. brucei and T. cruzi
Trypanosoma cruzi
Picophytoplankton Prochlorococcus spp;
Synechococcus spp
 
Plants Arabidopsis thaliana  
Phalaris arundinacea
Solidago canadensis
Yeast Candida albicans
Saccharomyces cerevisiae
Zebra fish Zebra fish organs

Customer stories

See how Dr. Bruno Sainz from Madrid Autónoma University describes his no-clog experience.  Learn the benefits of Attune technology to analyze tumor masses.