Online and downloadable materials for Attune Flow Cytometers

The tools, documents, downloads, videos, citations, customer testimonials, and other resources in this section will help you get the most out of your Attune Flow Cytometer.


FREE—Flow Cytometry Panel Design Service and Panel Builder Tool

Our scientists will design panels for you as many times you need—free of charge, no product purchase needed.

Fluorescence Spectra Viewer

Assess the spectral compatibility of dyes and probes for experiments that utilize fluorescence detection techniques.

Quick guides to fluorophore and reagent selection

Select the right fluorophores and reagents for your application with these Quick Guides.

Easily upgrade your flow cytometry data with brightfield images using the Attune CytPix Flow Cytometer

Join Dr. Cora Chadick, as she demonstrates how easy it is to start collecting imaging flow cytometry data using the new Invitrogen Attune CytPix Flow Cytometer. Watch as she walks through the simple workflow, instrument setup, parameter optimization, data collection, and data analysis steps.

Documents and downloads

Automated image analysis with flow cytometry


No-wash, no lyse


Fluorescent protein detection

Cell health

Resources for the original Attune Flow Cytometer

First-generation Attune Flow Cytometer support

Find technical resources for the original first-generation Invitrogen Attune Flow Cytometer, including manuals, reagent selection tables, software download information, and ordering information for consumables and accessories for your instrument.


Special guides

Flow Cytometry Protocols Handbook

Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping and data analysis strategies.


Flow and Sorting Article Collection

Get the latest in flow cytometry and cell sorting innovation, through these collection of articles.


Investigating flow cytometry instrument capabilities to maximize your research

The 40-page Flow Cytometer Evaluation Guide will enable a better understanding of the components and capabilities of various flow cytometers for objective comparison of instruments from several manufacturers.


Fluorophore and reagent selection guide for flow cytometry

A handy reference poster featuring the broad range of our dyes and labeling reagents available

Key features:

  • Recommended dyes for your flow cytometer based on lasers and detection channels settings.
  • Dye recommendation for UV, Violet, Blue, Green, Yellow, Red and Near IR lasers.
  • Checkout our specials-Brilliant Ultra Violet, Brilliant Violet and NovaFluor dyes; more dyes for spectral needs.

Multicolor Flow Cytometry Technical Resource Guide 

This technical resource guide describes the compensation and fixation considerations for blue, red and violet laser dyes. In addition, the guide details dyes and viability detection reagents for the each of these lasers. 


The Molecular Probes Handbook—A Guide to Fluorescent Probes and Labeling Technologies

The most complete reference on fluorescence labeling and detection available, this resource features extensive references and technical notes, and contains over 3,000 technology solutions representing a wide range of biomolecular labeling and detection reagents. 

Guided Learning

Molecular Probes School of Fluorescence

Developed by our in-house bench scientists this guided learning is designed to help you rapidly learn the basics of fluorescence, imaging and flow cytometry.

Visit the Flow Learning Center

Our flow cytometry application Learning Center has resources to help you succeed in planning and performing your experiments.

Fundamentals of Flow Cytometry

This guided learning module covers an introduction to flow cytometry and goes over the basics of the flow cytometer and how it works.


The Attune CytPix Flow Cytometer enables you to gain more data with automated image analysis

Improve accuracy in your flow cytometry data analysis with the Attune CytPix Flow Cytometer

Save time by combining two workflows with the Attune CytPix Flow Cytometer

Attune CytPix How-to Video

Simplifying GxP Compliance from Acquisition to Final Result with the Attune Cytometric Software™ and FCS Express™

Attune NxT Flow Cytometer Demo Video

Meet The Inventor

Our Meet the Inventor video series highlights all functional areas of the Attune Flow Cytometers.

Best practices to overcome common flow cytometry challenges

Design features of the Attune NxT Flow Cytometer

Modular Design and Flexibility of the Attune NxT Flow Cytometer

Fluidics of the Attune NxT Flow Cytometer

Optics of the Attune NxT Flow Cytometer

Software of the Attune NxT Flow Cytometer

Attune Software how-to videos

How to overlay data on the Attune NxT Flow Cytometer software

How to import a sample list on the Attune NxT Flow Cytometer software

How to set up compensation on the Attune NxT Flow Cytometer software

Setting up a new experiment using icons on the Attune NxT Flow Cytometer software

How to create a heat map using Attune NxT Flow Cytometer software

Attune published reference database

Our Reference Article Database allows you to easily perform keyword searches (i.e., author, cell type, species etc.) to see the latest peer-reviewed publications featuring the award-winning Attune Flow Cytometers from over 600 peer-reviewed scientific publications.

Customer stories

See what our customers are saying about Attune Flow Cytometers.

“We’ve been very comfortable working with Thermo Fisher Scientific for the last four years. We have a collaboration arrangement that goes back over three and a half years and has taken us from initial, in some cases, accidental/serendipitous discoveries, to a point where the technology we’re working on is being adopted by internationally respected reference laboratories overseas. That’s very unusual for a group based in somewhere as geographically isolated as Western Australia. This collaboration is a very unusual one, at least, for us locally and has been a very productive one.”

- Tim Inglis,


School of Medicine

University of Western Australia, PathWest Laboratory Medicine

“One of the problems that everyone is familiar with who works in flow cytometry is clogging. Clogging is a thing of the past with this instrument. You have so many samples that you have to run, you can’t wait between samples to clear everything out.”

- Bruno Sainz,

CLIP Investigator

Universidad Autónoma de Madrid, Madrid, Spain

"I can't believe how quickly and easy is to collect a large body of empirical evidence on the Attune NxT to fully support our hypothesis."

- Jordi Petriz, PhD

Group Leader, Functional Cytomics Group

Josep Carreras Leukaemia Research Institute, Barcelona, Spain

“This Attune NxT Small Particle SSC Filter has greatly increased the sensitivity of the system.  It really does make a huge difference.”

Steve McClellan,


Mitchell Cancer Institute

University of South Alabama

“So after evaluating several platforms, we chose the NxT because of its speed. It is able to analyze cells at a much higher rate, with very good precision, and it allows us to answer questions that other systems have trouble with, especially in the exosome field. The high resolution has been very beneficial in helping us resolve individual exosomes which are extremely small compared to other types of cells.”

Steve McClellan,


Mitchell Cancer Institute

University of South Alabama

“The analysis of minor population cells, such as the analysis of progenitor cells, is essential for immunology research today. As the sample volume inevitably increases and samples are so valuable, we need to use many fluorescently labeled antibodies in a single experiment. The Attune NxT Flow Cytometer that we primarily used in this case can detect up to a maximum of 14 colors using four colored lasers. I consider it to be a researcher-friendly system.” 

- Dr. Takashi Satoh,

IFReC Assistant Professor

Osaka University, Japan

“What I like most about the Attune NxT [Flow Cytometer] is its accuracy and its speed of processing. I could list a range of other things that I like about it because we are building up that list as we discover more aspects of its functions, but the core things are speed and accuracy that are not available with hydrodynamic flow cytometers.”

-Tim Inglis,


School of Medicine

University of Western Australia, PathWest Laboratory Medicine

“What we like about the Attune NxT in our lab, is the speed and effectiveness it provides for our cytokine and hormone bead-based assays. We run both 96 and 384 well bead assays, typically 10-15 plex, and the Attune NxT is a real workhorse. We combine it with our MPLEX software which reduces a complete plate of data in about a minute.”

- J. Paul Robinson,


Purdue University Cytometry Labs

home of the Purdue Discussion List

“The Attune NxT autosampler allowed us to process multiple 96-well plates in a day, enabling high throughput experiments that could not be performed on other flow cytometers.”

- James Hemphill, PhD

Senior Scientist


“The Attune NxT software is very user friendly and intuitive, significantly reducing training time required to get new users up-and-running.”

- James Hemphill, PhD

Senior Scientist


“The Attune NxT is a very well-built instrument, we are very happy with the Attune NxT. The automated Autosampler is a real plus when it is combined with the acoustic focusing because it allows you to count very diluted samples fast. It’s very precise and gives reliable event counting.”

- Hervé Luche, PhD,

Scientific Director


the Centre for ImmunoPHEnomics, Marseille, France

“I would highly recommend the Attune NxT cytometer.  It has several advantages including speed and ease of use, and it rarely clogs!  They also have an excellent plate/tube loader.  The best thing is that you can start with only one laser and easily field upgrade in just one afternoon, to add more in the future.”

- Steve McClellan,


Mitchell Cancer Institute

University of South Alabama