Conceptual art of a bottle of Gibco dialyzed FBS over a cell

Dialyzed FBS is manufactured using a process that significantly reduces the concentrations of small (low molecular weight) molecules such as amino acids, hormones, and cytokines while avoiding the precipitation of serum proteins.

Dialyzed fetal bovine serum is recommended for special applications where the normal concentrations of small molecules in FBS may be of concern, for example, in studies requiring higher levels of control over certain small molecules in the cell culture environment.

Dialyzed FBS vs FBS

Whereas standard FBS will contain higher concentrations of small molecules (e.g., amino acids, hormones, cytokines), the careful filtration process used to produce dialyzed FBS greatly reduces the concentrations of these molecules, facilitating more precise control of nutrients and cell signaling molecules in the cell culture medium.

Why researchers choose dialyzed FBS

Watch to discover how the depletion of small molecules in dialyzed FBS allows researchers to control for molecules of interest in their cell culture experiments.

Features of Gibco dialyzed fetal bovine serum

  • Dialyzed by tangential flow filtration (MW 10,000 cut-off)
  • Dialyzed against 0.15 M NaCl until glucose <5 mg/dL
  • Performance tested for cloning and plating efficiency
  • Available in the innovatively designed, aliquot-free Gibco One Shot FBS 50 mL bottle
Artistic rendering of a spheroid of GFP-expressing cells

Research areas using dialyzed FBS*

  • Proteomics (mass spectrometry)
  • Mitochondrial function studies
  • Glucose/amino acid deprivation studies
  • Isotope labeling
  • Cellular signaling and reporter assays

* These results are based on a review of approximately 10,000 publications using query terms based on the six specialty FBS products offered by Thermo Fisher Scientific. These terms were generated by the MeSH (medical subject headings) taxonomy based on the full text of the paper.

Order Gibco dialyzed FBS

Publications using Gibco dialyzed FBS

For Research Use or Further Manufacturing Use only. Serum and blood proteins are not for direct administration into humans or animals.

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