Rapid, economical alternative to subcloning yourself
Get your gene ready to use in your downstream applications. We can subclone your synthetic gene into any vector you send us. Even complex cloning projects with multiple open reading frames are no problem—for example, large double-gene vectors expressing monoclonal antibodies.
Using an alternative production procedure with pre-prepared expression vectors, GeneArt™ can clone your synthesized genes directly into selected expression plasmids, saving 4-5 business days compared to the standard subcloning workflow for expression-ready constructs with subcloning into pMx series vector first.
This Express cloning option is different from the still available classical subcloning service after gene synthesis (see table below). As deliverable, you will receive expression-ready genes—your synthesized gene in the selected expression vector, no additional copy in a pMx cloning vector will be provided.
Genes qualifying for Express cloning must be <5kb and not complex (optimization of your sequence can reduce complexity).
The following vectors are currently available for Express Cloning and can be directly selected during ordering (more vectors will be included for Express cloning over time):
Figure 1. Express cloning directly into an expression vector.
Figure 2. Classical subcloning from pMx into an expression vector.
*Expression vector example. Multiple choices for vector available.
We have substantial experience using Gateway™ recombination cloning technology, and the GeneArt Subcloning Service can be used for subcloning your synthetic gene into any vector you choose, including novel vectors built using the GeneArt Plasmid Construction Service. Focus on your results and leave the subcloning to GeneArt Services.
Choose “Subcloning (SC)” when setting up your project online
The basic subcloning service includes subcloning your DNA insert into the vector you specify. You’ll receive:
- 5 µg of plasmid DNA containing your insert
- 100% sequence verification