Fast, convenient DNA electrophoresis

E-Gel precast gels are bufferless agarose gels with electrodes embedded in the agarose matrix. Each precast agarose gel contains an ion generating system, a pH balancing system, and DNA stain packaged inside a transparent plastic cassette. It also contains two ion exchange matrices (IEMs) that are in contact with the gel and electrodes. The IEMs supply a continuous flow of ions throughout the precast gel resulting in a sustained electric field required for running the gel.

Features of E-Gel precast agarose gels

  • Ready-to-use—no gel preparation or additional buffers required
  • Safer analysis—choose E-Gels containing SYBR Safe DNA stain, a safer alternative to ethidium bromide
  • High performance—detect 2–3 ng of sample DNA in less than 30 minutes
  • Wide variety of applications—large selection of agarose percentages, well formats, and throughput capacities to suit your applications
     

See E-Gel precast gel comparison tables

e-gel-precast-agarose-gels-with-sybr-safe

E-Gel precast agarose gels with SYBR safe gel stain

Experience safer analysis without gels to pour, buffers to make, or chemical staining. Invitrogen E-Gel Precast Agarose Gels with SYBR Safe Gel Stain are designed for safer, more convenient DNA electrophoresis. Use the selection guide below to find precast gels containing SYBR safe gel stain by application.

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E-Gel precast agarose gel selection guide

E-Gel precast agarose gels are available in a variety of agarose percentages, well formats, and throughput capacities to suit many applications. Use the guide provided below to choose which precast agarose gel works best for the application in consideration.

E-Gel precast gels for low throughput DNA electrophoresis

For general purpose DNA electrophoresis or high sensitivity and fast separation; there is an E-Gel precast gel suited for many low throughput agarose gel electrophoresis applications.

GoalGeneral purposeHigh sensitivity and faster separation
Precast gel typesE-GelE-Gel EX
StainSYBR Safe DNA stainSYBR Gold II DNA stain
Run time13–40 min5–20 min
Number of wellsSingle row of 11 wells22 wells in a double comb designSingle row of 11 wells22 wells in a double comb design
Agarose %
  • 1.0%
  • 2.0%
  • 4.0%
  • 1.0%
  • 2.0%
  • 1.0%
  • 2.0%
  • 4.0%
  • 1.0%
  • 2.0%
  • Resolution10 bp–5 kb50 bp–5 kb10 bp–5 kb50 bp–5 kb
    Sample volume20 µL20 µL20 µL20 µL

    E-Gel 48- and 96-well precast agarose gels for high-throughput DNA electrophoresis

    E-Gel 48- and 96-well agarose gels are ideal for high-throughput electrophoresis and analysis of PCR products, plasmid preparations, and restriction digests. E-Gel 48 gels include two rows each containing 24 samples and two molecular weight marker wells with a 3.2 cm length. E-Gel 96 gels contain 96 sample lanes and eight marker lanes, staggered to provide a 1.6 cm run length with resolution between 100 bp and 10 kb. This staggered-well format is compatible with most robotic loading systems.

    GoalHigh throughput
    Precast gel typeE-Gel 48-well gelsE-Gel 96-well gels
    Number of wells48 sample wells and 4 marker wells96 sample wells and 8 marker wells
    StainSYBR Safe DNA stain or Ethidium bromideSYBR Safe DNA stain or Ethidium bromide
    Agarose %
  • 1.0% SYBR
  • 2.0% SYBR
  • 4.0% SYBR
  • 1.0% EtBr
  • 2.0% EtBr
  • 4.0% EtBr
  • 1.0% SYBR
  • 2.0% SYBR
  • 1.0% EtBr
  • 2.0% EtBr
  • Resolution10 bp–10 kb100 bp–10 kb
    Run time17–25 min12–17 min
    Sample volume15 µL20 µL

    E-Gel precast gels for cloning

    Using E-Gel CloneWell II agarose gels with the E-Gel Power Snap Electrophoresis System eliminates UV damage and improves cloning efficiency. E-Gel CloneWell II agarose gels are double-comb gels designed for the sample to be loaded into the top well, electrophoresed, and bands can then be retrieved once they migrate into the collection well. No additional purification is required.

    GoalHigh efficiency cloning
    Precast gel typeE-Gel CloneWell II
    Number of wellsTwo rows of 7 wells (for 7 samples)
    StainSYBR Safe DNA stain
    Agarose %0.8%
    Resolution100 bp–6 kb
    Run time13–28 min
    Sample volume20–25 µL

    E-Gel precast gels for NGS library preparation

    For NGS library prep, the ability to recover a purified DNA fragment is critically important. Choose the E-Gel precast agarose gel that suits the throughput and precision needs of your NGS sample prep workflow.

    GoalsPrecise DNA fragment library size selectionHigh range fragment library separation
    Precast gel typesE-Gel SizeSelect IIE-Gel NGS
    Number of wellsTwo rows of 7 wells (for 7 samples)Single row of 11 wells
    StainSYBR Gold stainSYBR Safe DNA stain
    Agarose %2.0%0.8%
    Resolution50 bp–2 kb800 bp–10 kb
    Run time8–20 min26–32 min
    Sample volume20–25 µL20 µL

    E-Gel precast gels for low throughput DNA electrophoresis

    For general purpose DNA electrophoresis or high sensitivity and fast separation; there is an E-Gel precast gel suited for many low throughput agarose gel electrophoresis applications.

    GoalGeneral purposeHigh sensitivity and faster separation
    Precast gel typesE-GelE-Gel EX
    StainSYBR Safe DNA stainSYBR Gold II DNA stain
    Run time13–40 min5–20 min
    Number of wellsSingle row of 11 wells22 wells in a double comb designSingle row of 11 wells22 wells in a double comb design
    Agarose %
  • 1.0%
  • 2.0%
  • 4.0%
  • 1.0%
  • 2.0%
  • 1.0%
  • 2.0%
  • 4.0%
  • 1.0%
  • 2.0%
  • Resolution10 bp–5 kb50 bp–5 kb10 bp–5 kb50 bp–5 kb
    Sample volume20 µL20 µL20 µL20 µL

    E-Gel 48- and 96-well precast agarose gels for high-throughput DNA electrophoresis

    E-Gel 48- and 96-well agarose gels are ideal for high-throughput electrophoresis and analysis of PCR products, plasmid preparations, and restriction digests. E-Gel 48 gels include two rows each containing 24 samples and two molecular weight marker wells with a 3.2 cm length. E-Gel 96 gels contain 96 sample lanes and eight marker lanes, staggered to provide a 1.6 cm run length with resolution between 100 bp and 10 kb. This staggered-well format is compatible with most robotic loading systems.

    GoalHigh throughput
    Precast gel typeE-Gel 48-well gelsE-Gel 96-well gels
    Number of wells48 sample wells and 4 marker wells96 sample wells and 8 marker wells
    StainSYBR Safe DNA stain or Ethidium bromideSYBR Safe DNA stain or Ethidium bromide
    Agarose %
  • 1.0% SYBR
  • 2.0% SYBR
  • 4.0% SYBR
  • 1.0% EtBr
  • 2.0% EtBr
  • 4.0% EtBr
  • 1.0% SYBR
  • 2.0% SYBR
  • 1.0% EtBr
  • 2.0% EtBr
  • Resolution10 bp–10 kb100 bp–10 kb
    Run time17–25 min12–17 min
    Sample volume15 µL20 µL

    E-Gel precast gels for cloning

    Using E-Gel CloneWell II agarose gels with the E-Gel Power Snap Electrophoresis System eliminates UV damage and improves cloning efficiency. E-Gel CloneWell II agarose gels are double-comb gels designed for the sample to be loaded into the top well, electrophoresed, and bands can then be retrieved once they migrate into the collection well. No additional purification is required.

    GoalHigh efficiency cloning
    Precast gel typeE-Gel CloneWell II
    Number of wellsTwo rows of 7 wells (for 7 samples)
    StainSYBR Safe DNA stain
    Agarose %0.8%
    Resolution100 bp–6 kb
    Run time13–28 min
    Sample volume20–25 µL

    E-Gel precast gels for NGS library preparation

    For NGS library prep, the ability to recover a purified DNA fragment is critically important. Choose the E-Gel precast agarose gel that suits the throughput and precision needs of your NGS sample prep workflow.

    GoalsPrecise DNA fragment library size selectionHigh range fragment library separation
    Precast gel typesE-Gel SizeSelect IIE-Gel NGS
    Number of wellsTwo rows of 7 wells (for 7 samples)Single row of 11 wells
    StainSYBR Gold stainSYBR Safe DNA stain
    Agarose %2.0%0.8%
    Resolution50 bp–2 kb800 bp–10 kb
    Run time8–20 min26–32 min
    Sample volume20–25 µL20 µL


    Stylesheet for Classic Wide Template adjustments

    For Research Use Only. Not for use in diagnostic procedures.

    Stylesheet for Classic Wide Template adjustments