Bleaching. Ultraviolet treatment. Autoclaving. To the average molecular biologist, eliminating nuclease contamination, particularly ribonuclease (RNase) contamination, is a constant and somewhat annoying challenge. RNA is an especially sensitive and difficult analyte to work with, as it is readily degraded. Therefore, it is essential that labware, instruments, and surfaces involved in the purification of RNA are free from RNases. Even trace quantities of RNases can lead to lower yields from in vitro transcription reactions, degradation during RNA purification protocols, and variable results with qRT-PCR.
All surfaces, including benchtops, pipettes, glassware, and benchtop instruments, should be treated with a surface decontamination agent. We offer several surface decontamination products that are proven effective at eliminating RNase contamination from lab surfaces—RNaseZap Solution, RNaseZap Wipes, ElectroZap Solution, and RNase AWAY Reagent.
Product Selection Guide
|Surface Decontamination||RNaseZap Solution||
|RNase AWAY Reagent||
RNaseZap RNase Decontamination Products
Ribonucleic acid (RNA) is an especially sensitive and difficult molecule to work with because it is readily degraded. To help ensure success, steps must be taken to minimize nuclease contamination in RNA purification laboratories. Even trace quantities of RNase can lead to lower yields from in vitro transcription reactions, degradation during RNA purification protocols, and variable results with qRT-PCR.
Thermo Fisher Scientific offers several Ambion products in the RNaseZap family—RNaseZap Solution, RNaseZap Wipes, and ElectroZap Solution—that collectively provide a comprehensive approach to help ensure that work surfaces, pipettors, and equipment are RNase-free.
RNAseZap products offer:
- Excellent decontamination of dried-on RNase A (Figure 1)
- Better RNase A decontamination than competitive products (Figure 2)
Figure 1. Removal of Dried-on RNase Contamination. A 5 µg sample of RNase A was vacuum-dried onto the bottom of two microfuge tubes. One tube was then rinsed with water, and one tube was rinsed with RNaseZap solution. Two micrograms of total RNA was added to each of the microcentrifuge tubes, incubated at 37°C for 30 min, and assessed on a 1.5% agarose gel.
Figure 2. Comparison of Available Cleaning Products for the Removal of RNase Contamination. Mouse liver total RNA (2 µg) was added to microcentrifuge tubes precontaminated with RNase A and then cleaned with different commercially available RNase decontamination products: "a", "b", or RNaseZap solution. The tubes were then rinsed twice with water prior to the addition of the RNA in 20 µL of TE. The samples were incubated at 37°C for 30 min. Ten microliters of each sample was resolved on a 1.5% agarose gel (TAE) and visualized on a UV light box.
Surface Decontamination Support Materials
- How to Maintain an RNase-Free Lab - Technical Bulletin #180
- Nuclease and Protease Testing - Technical Bulletin #166
- RNase and DEPC Treatment: Fact or Laboratory Myth? - Technical Bulletin #178
- Working With RNA - Technical Bulletin #159
- Combat RNase Contamination in the Lab - TechNotes 11(2): We offer a complete selection of Ambion products to help prevent, detect, and eliminate RNase contamination in molecular biology experiments
- Detect RNases Before They Ruin Your Experiment - TechNotes 8(2): New 30-minute test for verifying that common lab solutions are RNase-free.
- Measuring RNase Activity - A Real-time Kinetic Analysis - TechNotes 8(4): Detect nuclease and standardize enzyme activity.
- RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control - TechNotes 12(3): A comparison of total RNase activities in eight different mouse tissues, with recommendations for the best RNA isolation methods.
- SUPERase•In: The Right Choice for Protecting Your RNA - TechNotes 8(2): New ribonuclease inhibitor that inhibits a broader range of RNases over a broader range of conditions than traditional RNase inhibitors.
- Which Water to Use? - TechNotes 11(4): Don't overlook the water used in the experiment when trying to pinpoint the source of RNase contamination.
- Your Data: RNase A Molecules are Used to Study the Structure of Amyloid-Like Fibrils: A summary of recently published data on protein refolding during the formation of amyloid fibrils and the retention of native protein function demonstrated through the use of our Ambion RNaseAlert technology.
For Research Use Only. Not for use in diagnostic procedures.