|Limit of detection||≤ 200 transcripts/assay well|
|Limit of quantitation||≤ 500 transcripts/assay well|
|Linear dynamic range||≥ 3.5 logs|
|Assay CV||≤ 10% intra-assay; ≤ 15% inter-assay|
|Compatible sample types||Cultured cells, bacteria, whole blood,
PAXgene blood or dried blood spots,
fresh/frozen tissues (animal or plant),
FFPE samples, purified RNA
|Assay format||96-well plate|
QuantiGene Singleplex Gene Expression Assay accuracy: excellent correlation with TaqMan data
To demonstrate the accuracy of the QuantiGene Singleplex Gene Expression Assay, measurements of twenty transcripts from two reference RNA samples were made and compared to TaqMan data. Reference RNA samples were human brain total RNA and universal human reference RNA as described in Canales et al. 2006. Nature Biotechnology 24(9):1115-1122. As shown below, excellent correlations in fold-change of RNA levels in the reference samples were seen between QuantiGene SinglePlex and TaqMan assays (R2 = 0.965). TaqMan data are from Canales et al. 2006 publication.
For Research Use Only. Not for use in diagnostic procedures.