Dye-Labeled Phosphoramidites

Thermo Fisher Scientific offers DyLight fluorescent–labeled phosphoramidites that feature absorption and emission spectra suitable for a broad range of applications, including microscopy and PCR. 

These phosphoramidites may be used on all automated DNA synthesizers using standard synthesis procedures

Our expertise includes the development of custom dye chemistries, linkers, and labeling strategies. Fluorescent tags can be incorporated at the 5′ end of an oligonucleotide using DyLight phosphoramidites with standard synthesis and deprotection procedures. In addition, these direct-labeled phosphoramidites can be used in research applications without the constraints of licensing and royalty fees.

DyLight-labeled Phosphoramidites

 DyLight DY547 CEPDyLight DY647 CEPDyLight DY677 CEP
Cat. No.SY6332SY6334SY6336
FormulaC38H54IN4O2PC47H63N4O5PSC55H67N4O5PS
Molecular weight756.74 g/mol827.07 g/mol927.18 g/mol
HPLC purity≥ 90% at 547 nm≥ 85% at 647 nm≥ 85% at 677 nm
IdentityUV-Vis spectrum conforms to that of the authentic sample
Shipping / Storage conditionDry ice / -20 °C
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DyLight Fluorescein-labeled Phosphoramidites

DyLight Fluorescein can be further purified to remove the 5-isomer and leaves ≥95% of the 6-isomer (FAM-6), making it ideal for applications requiring higher sensitivity.

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 DyLight FAM-6 CEPDyLight Fluorescein CEPDyLight HEX CEPDyLight TET CEP
Cat. No.27-160827-1606SY6342SY6341
Phosphoramidite DescriptionFluorescein dipivalate phosphoramidite, 6-isomer[(3′,6′-dipivaloylfluoresceinyl)-5,6-carboamidohexyl]-1-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite6-(4,7,2′,4′,5′,7′-Hexachloro-3′,6′-dipivaloylfluoresceinyl-6-carboxamido)-hexyl-1-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite5′-Tetrachloro-Fluorescein Phosphoramidite
FormulaC46H58N3O10P C46H58N3O10PC46H52N3O10Cl6PC46H54N3O10Cl4P
Molecular weight843.95g/mol843.94g/mol1050.63 g/mol981.73 g/mol
CAS / PubChem#204697-37-0 / 58114954N/A1360547-55-2877049-90-6
HPLC purity≥ 90%≥ 90%≥ 95%≥ 95%
31P NMR purity≥ 95% (6-isomer)85%N/AN/A
Shipping / Storage  conditionDry ice / -20 °C
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Process and supply chain control

  • A controlled raw material supply chain and established vendor partnerships ensure consistency and security of supply
  • All dye amidites are supplied with lot-specific quality control data including HPLC analysis
  • Processes are monitored from material procurement through final QC and product release, providing batch-to-batch reproducibility

Custom services

  • Custom manufacturing services are available to assist in the development or scale-up of phosphoramidite compounds
  • Specialty phosphoramidites can be manufactured with base or sugar (2′, 3′ or 5′ positions) modifications

Flexible packaging

  • Common oligo synthesizer pack sizes are available for immediate delivery
  • Custom and bulk packaging are also available. Contact us to learn more.

 

Not finding what you are looking for? We also offer DNA phosphoramiditesRNA phosphoramiditesspecialty phosphoramidites2′-modificationslinker and spacer phosphoramidites, and succinates and CPG support synthesis supportsContact us to learn more.