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Dye-Labeled Phosphoramidites |
Thermo Fisher Scientific offers DyLight fluorescent–labeled phosphoramidites that feature absorption and emission spectra suitable for a broad range of applications, including microscopy and PCR.
These phosphoramidites may be used on all automated DNA synthesizers using standard synthesis procedures
Our expertise includes the development of custom dye chemistries, linkers, and labeling strategies. Fluorescent tags can be incorporated at the 5′ end of an oligonucleotide using DyLight phosphoramidites with standard synthesis and deprotection procedures. In addition, these direct-labeled phosphoramidites can be used in research applications without the constraints of licensing and royalty fees.
For more than 40 years, our OEM and custom capabilities in research, development, and manufacturing of nucleic acids continue to be key resources for our global partners at molecular diagnostic, pharmaceutical, and life science research companies.
DyLight-labeled Phosphoramidites
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DyLight DY547 CEP
DyLight DY647 CEP
DyLight DY677 CEP
| DyLight DY547 CEP | DyLight DY647 CEP | DyLight DY677 CEP | |
|---|---|---|---|
| Cat. No. | SY6332 | SY6334 | SY6336 |
| Formula | C38H54IN4O2P | C47H63N4O5PS | C55H67N4O5PS |
| Molecular weight | 756.74 g/mol | 827.07 g/mol | 927.18 g/mol |
| HPLC purity | ≥ 90% at 547 nm | ≥ 85% at 647 nm | ≥ 85% at 677 nm |
| Identity | UV-Vis spectrum conforms to that of the authentic sample | ||
| Shipping / Storage condition | Dry ice / -20 °C | ||
| Request a quote | |||
DyLight Fluorescein-labeled Phosphoramidites
DyLight Fluorescein can be further purified to remove the 5-isomer and leaves ≥95% of the 6-isomer (FAM-6), making it ideal for applications requiring higher sensitivity.
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DyLight FAM-6 CEP
DyLight Fluorescein CEP
DyLight HEX CEP
DyLight TET CEP
| DyLight FAM-6 CEP | DyLight Fluorescein CEP | DyLight HEX CEP | DyLight TET CEP | ||
|---|---|---|---|---|---|
| Cat. No. | 27-1608 | 27-1606 | SY6342 | SY6341 | |
| Phosphoramidite Description | Fluorescein dipivalate phosphoramidite, 6-isomer | [(3′,6′-dipivaloylfluoresceinyl)-5,6-carboamidohexyl]-1-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | 6-(4,7,2′,4′,5′,7′-Hexachloro-3′,6′-dipivaloylfluoresceinyl-6-carboxamido)-hexyl-1-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | 5′-Tetrachloro-Fluorescein Phosphoramidite | |
| Formula | C46H58N3O10P | C46H58N3O10P | C46H52N3O10Cl6P | C46H54N3O10Cl4P | |
| Molecular weight | 843.95g/mol | 843.94g/mol | 1050.63 g/mol | 981.73 g/mol | |
| CAS / PubChem# | 204697-37-0 / 58114954 | N/A | 1360547-55-2 | 877049-90-6 | |
| HPLC purity | ≥ 90% | ≥ 90% | ≥ 95% | ≥ 95% | |
| 31P NMR purity | ≥ 95% (6-isomer) | 85% | N/A | N/A | |
| Shipping / Storage condition | Dry ice / -20 °C | ||||
| Request a quote | |||||
Process and supply chain control
- A controlled raw material supply chain and established vendor partnerships ensure consistency and security of supply
- All dye amidites are supplied with lot-specific quality control data including HPLC analysis
- Processes are monitored from material procurement through final QC and product release, providing batch-to-batch reproducibility
Custom services
- Custom manufacturing services are available to assist in the development or scale-up of phosphoramidite compounds
- Specialty phosphoramidites can be manufactured with base or sugar (2′, 3′ or 5′ positions) modifications
Flexible packaging
- Common oligo synthesizer pack sizes are available for immediate delivery
- Custom and bulk packaging are also available. Contact us to learn more.
Not finding what you are looking for? We also offer DNA phosphoramidites, RNA phosphoramidites, specialty phosphoramidites, 2′-modifications, linker and spacer phosphoramidites, and succinates and CPG support synthesis supports. Contact us to learn more.
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