TheraPure DNA phosphoramidites were engineered specifically for oligonucleotide manufacturers requiring tightly controlled impurity profiles, strictly controlled processes with thorough documentation, and a rigorous process change control. With our in-house high-definition, advanced analytical capabilities including LC-MS, GC, HPLC, 31P NMR and
For more than 40 years, our OEM and custom custom capabilities in research, development, and manufacturing of nucleic acids continue to be key resources for our global partners at molecular diagnostic, pharmaceutical, and life science research companies.
DNA phosphoramidites by synthesizer compatibility
| Synthesizer | Applied Biosystems /AKTA | Expedite | MerMade | Bulk |
|---|---|---|---|---|
| TheraPure | Bz-dA | Bz-dA | Bz-dA | Contact us |
| Bz-dC | Bz-dC | Bz-dC | ||
| iBu-dG | iBu-dG | iBu-dG | ||
| dT | dT | dT | ||
| Standard grade | Bz-dA | Bz-dA | Bz-dA | Contact us |
| Bz-dC | Bz-dC | Bz-dC | ||
| iBu-dG | iBu-dG | iBu-dG | ||
| dT | dT | dT | ||
| Fast Deprotection (mild) | PAC-dA | DMF-dG | DMF-dG | Contact us |
TheraPure DNA Phosphoramidites
- Raw materials and intermediates are always QC tested using analytical technologies (listed above)
- State-of-the-art purification methods utilized to obtain the highest amidite purity
- Overall, high purity and tight critical impurity level control yields excellent batch-to-batch reproducibility
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dA(Bz) CEP
dC(Bz) CEP
dG(iBu) CEP
dT CEP
| dA(Bz) CEP | dC(Bz) CEP | dG(iBu) CEP | dT CEP | |
|---|---|---|---|---|
| Cat. No. | 27-2030 | 27-2032 | 27-2034 | 27-2036 |
| Formula | C47H52N7O7P | C46H52N5O8P | C44H54N7O8P | C40H49N4O8P |
| Molecular weight | 857.95 g/mol | 833.93 g/mol | 839.92 g/mol | 744.82 g/mol |
| CAS # | 98796-53-3 | 102212-98-6 | 93183-15-4 | 98796-51-1 |
| HPLC purity* | ≥ 99% | |||
| 31P NMR purity* | ≥ 99% | |||
| Solution Clarity | Free from undissolved particulate at 0.2M solution in ACN | |||
| Appearance | White to pale yellow powder | |||
| Residual Solvent | ≤ 3.0% total % by weight | |||
| Shipping/Storage condition | Ambient / –20 °C | |||
| Water content | ≤ 0.3% | |||
| Material Origin | Non-animal source (no TSE/BSE risk) | |||
* For more specification details, contact us.
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Standard DNA Phosphoramidites
Traditional base protection methods are used to ensure quality and reliability for routine oligonucleotide synthesis.
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dA(Bz) CEP
dC(Bz) CEP
dG(iBu) CEP
dT CEP
| dA(Bz) CEP | dC(Bz) CEP | dG(iBu) CEP | dT CEP | |
|---|---|---|---|---|
| Cat. No. | 27-1730 | 27-1732 | 27-1734 | 27-1736 |
| Formula | C47H52N7O7P | C46H52N5O8P | C44H54N7O8P | C40H49N4O8P |
| Molecular weight | 857.95 g/mol | 833.93 g/mol | 839.92 g/mol | 744.82 g/mol |
| CAS # | 98796-53-3 | 102212-98-6 | 93183-15-4 | 98796-51-1 |
| HPLC purity | ≥ 98.0% | |||
| 31P NMR purity | ≥ 98.0% | |||
| Water content (KF Method) | ≤ 0.3% | |||
| Shipping / Storage condition | Ambient / –20 °C | |||
| Solution Clarity | Free from undissolved particulate at 0.2M solution in ACN | |||
| Material Origin | Non-animal source (no TSE/BSE risk) | |||
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Fast Deprotect DNA Phosphoramidites
Fast Deprotect DMF-dG, iPrPAC-dG, PAC-dA, Ac-dC, and iBu-dC phosphoramidites are ideal for applications requiring mild deprotection to preserve the integrity of oligonucleotides containing sensitive dyes, labels, or other modifications, which may be unstable with prolonged exposure to strong alkaline conditions. Fast Deprotect phosphoramidites permit the removal of base protecting groups under mild or accelerated conditions without the need for special reagents.
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iPrPAC-dG CEP
PAC-dA CEP
Ac-dC CEP
DMF-dG CEP
| iPrPAC-dG CEP | PAC-dA CEP | Ac-dC CEP | DMF-dG CEP | IBU-dC CEP | |
|---|---|---|---|---|---|
| Cat. No. (TheraPure) | N/A | N/A | 27-2027 | N/A | N/A |
| Cat. No. (standard) | 27-1726 | 27-1723 | 29-1727 | 27-1737 | 27-1725 |
| Formula | C51H60N7O9P | C48H54N7O8P | C41H50N5O8P | C43H53N8O7P | C43H54N5O8P |
| Molecular weight | 946.05 g/mol | 887.96 g/mol | 771.8 g/mol | 824.9 g/mol | 839.92g/mol |
| CAS # | 150065-82-0 | 110543-74-3 | 154110-40-4 | 330628-04-1 | 110522-84-4 |
| HPLC purity |(TheraPure)* | ≥ 98.0% | ≥ 98.0% | ≥ 98% | (≥ 99%) | ≥ 98.0% | ≥ 98.0% |
| 31P NMR purity | (TheraPure)* | ≥ 98.0% | ≥ 98.0% | ≥ 98% | (≥ 99%) | ≥ 98.0% | ≥ 98.0% |
| Shipping / Storage condition | Ambient / –20 °C | ||||
| Material Origin | Non-animal source (no TSE/BSE risk) | ||||
* For more specification details, contact us.
Order now
Process and supply chain control
- Processes are monitored from material procurement through final QC and product release, providing batch-to-batch reproducibility
- A controlled raw material supply chain and established vendor partnerships ensure consistency and security of supply
- Purity of each DNA phosphoramidite lot is assessed by HPLC and 31P NMR analyses
- Rigorously controlled manufacturing processes minimize levels of reactive P(III) impurities which could be incorporated into the oligonucleotide and result in the accumulation of by-products
Custom services
- Custom manufacturing services are available to assist in the development or scale-up of phosphoramidite compounds
- Specialty phosphoramidites can be manufactured with base or sugar (2′, 3′ or 5′ positions) modifications
Flexible packaging
- Common oligo synthesizer pack sizes are available for immediate delivery
- Custom and bulk packaging are also available. Contact us to learn more.
Not finding what you are looking for? We also offer RNA phosphoramidites, specialty phosphoramidites, dye-labeled phosphoramidites, 2′-modifications, linker and spacer phosphoramidites, and succinates and CPG support synthesis supports. Contact us to learn more.
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For Research Use Only. Not for use in diagnostic procedures.
