2′ Modified RNA Phosphoramidites

Modifications at the 2′ position of the ribose ring are commonly used to help increase oligonucleotide stability and improve resistance to nuclease activity in vivo. Thermo Scientific 2'-modified RNA phosphoramidites ensure high level quality and reliability for all oligonucleotide synthesis needs. Phosphoramidites are manufactured to precise standards to reduce levels of critical impurities and supports a broad range of nucleic acid-based applications such as antisense, siRNA, gene design, and genome editing, including CRISPR-cas9 gRNA.

2’ Modified RNA phosphoramidites by synthesizer compatibility

TheraPure 2’-MOE Phosphoramidites

RNA oligonucleotides synthesized using 2′-MOE modifications phosphoramidites have shown to be more nuclease resistant, with lower toxicity, and slightly increased hybridization affinities, making them well suited for therapeutic in vivo applications, such as antisense (ASO), siRNA, and aptamers. 

  • Tightly controlled, critical impurities (eg. M-11)
  • Suitable for use with DNA and/or RNA phosphoramidites
  • Reliable, high quality oligonucleotide synthesis

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 2′-MOE-A CEP2′-MOE-5mC CEP2′-MOE-G CEP2′-MOE-T CEP
Cat. No.27-101927-102027-102227-1021
FormulaC50H58N7O9PC50H60N5O10PC47H60N7O10PC43H55N4O10P
Molecular weight932.03 g/mol922.0 g/mol914.01 g/mol819.0 g/mol
CAS #251647-53-7163759-94-2251647-55-9163878-63-5
Solution ClarityFree from undissolved particulate at 0.2M solution in ACN
HPLC purity*

 ≥ 99%

31P NMR purity*≥ 99%
Shipping / Storage  conditionAmbient / –20 °C
Water content≤ 0.3% (by KF Method)
Total residual solvents≤ 4.0%
Material OriginNon-animal source (no TSE/BSE risk)

* For more specification details, contact us.

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2'-Fluoro Phosphoramidites - TheraPure and standard

RNA oligonucleotides synthesized using 2′-Fluoro Bz A, iBu G, Ac C, and U phosphoramidites have increased thermal stability of RNA duplexes and nuclease resistance of single-stranded RNA. These phosphoramidites can be incorporated into custom siRNA oligonucleotides for use in gene-targeting and suppression studies.

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 2′-Fluoro-Bz-A-CEP2′-Fluoro-iBu-G-CEPTheraPure 2′-Fluoro-Ac-C-CEPTheraPure 2′-Fluoro-U-CEPTP 2'-Fluoro-Ac-G-CEP
Cat. No.27-160127-160727-160427-1602SY5410*
FormulaC47H51FN7O7PC44H53FN7O8PC41H49N5FO8PC39H46FN4O8PC42H49FN7O8P
Molecular weight875.92 g /mol857.4 g/mol789.8 g/mol748.8 g/mol829.87 g/mol
CAS #NA144089-97-4159414-99-0146954-75-8N/A
AppearanceWhite to light yellow powder
HPLC purity*>98%
31P NMR purity*>98%
ResidualSolventN/A≤ 3.0% total % by weight solvent 
Solution ClarityFree from undissolved particulate at 0.2M solution in ACN
Shipping / Storage conditionAmbient / –20 °C
Water content≤ 0.3%
Material OriginNon-animal source (no TSE/BSE risk)

* For more specification details, contact us.

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2'-OMe Phosphoramidites - TheraPure and standard

TheraPure (and standard) 2′-OMe phosphoramidites are presented in the table below.  For faster deprotection times, consider reviewing the PAC chemistries in combination with 2'-OMe U (no base protection) and Ac-rC.   

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 2′-OMe-Bz-A-CEP2′-OMe-U-CEP2′-OMe-iBu-G-CEP2′-OMe-Ac-C-CEP
TheraPure Cat. No.27-204227-204427-204627-2043
Cat. No.27-184227-182527-184627-1823
FormulaC48H54N7O8PC40H56N7O9PC45H56N7O9PC42H52N5O9P
Molecular weight887.97 g/mol760.8 g/mol869.9 g/mol801.86 g/mol
CAS #110782-31-5110764-79-9150780-67-9199593-09-4
AppearanceColor intensity must not exceed Pantone #P1-4U or #P4-2U
HPLC purity (TheraPure)≥ 98.0% | (>99%) *
M-11 Impurity≤ 0.01%
31P NMR purity (TheraPure)≥ 98.0% | (>99%) *
Shipping / Storage  conditionAmbient / –20 °C
Water content ≤ 0.2% (KF Method)
Material OriginNon-animal source (no TSE/BSE risk)

* For more specification details, contact us.

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2'-OMe Phosphoramidites - FAST deprotection

Utilize PAC-A and iPrPAC-G with Ac-C and U to minimize deprotection times.  Acetylated amidites (such as Ac-G, SY5400) are ideal for shortening deprotection times for longmers. 

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 2′-OMe-PAC-A-CEP2′-OMe-iPrPAC-G-CEPTheraPure 2'-OMe-Ac-G-CEP
Cat. No.27-182227-1826SY5400
FormulaC49H56N7O9PC52H62N7O10PC43H52N7O9P
Molecular weight918.0 g/mol976.1 g/mol841.9 g/mol
CAS #128219-82-9NA909033-40-5
HPLC purity≥ 98.0%≥ 98.0%≥ 99%
31P NMR purity≥ 98.0%≥ 98.0%≥ 99%
HazardousNo
Shipping / Storage  conditionAmbient / –20 °C
Material OriginNon-animal source (no TSE/BSE risk)
C52H62N7O10P

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Process and supply chain control

  • Processes are monitored from material procurement through final QC and product release, providing batch-to-batch reproducibility.
  • A controlled raw material supply chain and established vendor partnerships ensure consistency and security of supply
  • Purity of each 2'-modified phosphoramidite lot is assessed by HPLC and 31P NMR analyses
  • Rigorously controlled manufacturing processes minimize levels of reactive P(III) impurities which could be incorporated into the oligonucleotide and result in the accumulation of by-products

Custom services

  • Custom manufacturing services are available to assist in the development or scale-up of phosphoramidite compounds
  • Specialty phosphoramidites can be manufactured with base or sugar (2′, 3′ or 5′ positions) modifications

Flexible packaging

  • Common oligo synthesizer pack sizes are available for immediate delivery
  • Custom and bulk packaging are also available. Contact us to learn more.

 

Not finding what you are looking for? We also offer DNA phosphoramidites, RNA phosphoramidites, specialty phosphoramidites, dye-labeled phosphoramidites, linker and spacer phosphoramidites, and succinates and CPG support synthesis supports. Contact us to learn more.