SureQuant Targeted Mass Spec Assay Kits

The Thermo Scientific SureQuant kits enable multiplex immunoprecipitation to mass spectrometry (mIP-MS) for the simultaneous enrichment and quantitation of multiple total and phosphorylated proteins (13 in total, including 11 phosphorylated states) in the AKT/mTOR signaling pathway. Each multiplex panel contains two modules: 1) an IP and MS sample prep module which includes all the reagents necessary to immunoenrich AKT-mTOR pathway proteins and perform in-solution MS sample preparation and 2) an absolute or relative quantitation module, which includes a system suitability standard and AQUA Ultimate Heavy and/or Light Peptide mixtures.

  • Complete – includes all standards and reagents for the successful monitoring of the MS system performance, sample preparation and the absolute or relative quantitation of target proteins/peptides
  • Validated – antibodies, peptides and control lysate rigorously tested for specificity and successful quantitation of each target peptide
  • Multiplex – able to quantitate different multiplex targets (including isoforms and phosphorylated states) from the AKT-mTOR pathway
  • Flexible – modular format allows for immune-enrichment only, or in combination with relative or absolute quantitation

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Absolute quantitation of AKT/mTOR signaling pathway proteins using the SureQuant AKT Pathway Multiplex Panel (Absolute Quantitation) and SureQuant AKT Pathway (Phospho) Multiplex Panel (Absolute Quantitation)
Absolute quantitation of AKT/mTOR signaling pathway proteins using the SureQuant AKT Pathway Multiplex Panel (Absolute Quantitation) and SureQuant AKT Pathway (Phospho) Multiplex Panel (Absolute Quantitation). The panels were run on MCF7 and A549 cells treated with IGF1. AKT/mTOR pathway proteins were enriched through multiplex immunoprecipitation, then analyzed by nanoLC-PRM/MS on a Thermo Scientific QExactive HF-Orbitrap mass spectrometer. All targets were quantified using a standard curve generated for each target peptide included in the Quantitation Modules.
HeavyPeptide AQUA Custom Synthesis Service

Thermo Scientific HeavyPeptide AQUA Custom Synthesis Service provides isotopically labeled, AQUA-grade peptides for the relative and absolute quantitation of proteins.

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