Targeted DNA panels for any genome

If the Ion AmpliSeq ready-to-use panels don't match your gene targets, then simply select a subset of the targets or enter your specific genomic content online and we will design and create customized Ion AmpliSeq NGS panels just for you.

Creating and ordering custom gene panels is easy with the Ion AmpliSeq Designer—a free, online assay design tool that connects you directly to our assay design pipeline.  Panels can be designed against a number of preloaded genomes, or for any other organism by uploading your own FASTA file reference sequence.

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Preloaded reference genomes available

Custom panels are delivered in tubes of prepooled, multiplexed primers in ready-to-use concentrations.  You can add 384-well plates to your order, in which each well contains individual primer pairs in concentrated format.  For custom panels with ≤96 primer pairs, you will receive 2,250 total reactions.  For custom panels with >96 primer pairs, you will receive 9,000 total reactions.

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Ion AmpliSeq Designer performance specifications

Specifications*Current value
Input DNA amount per primer poolAs little as 1 ng
Available reference genomesCow, chicken, human, maize, mouse, pig, rice, sheep, soybean, tomato
Custom referencesUpload any reference genome to a password-protected cloud space
Primer Pairs per pool12 to 24,000
Target size1 kb to 5 Mb
Target design rate**>90%
Coverage uniformity†>85%

** Target design rate—bases covered by the insert region of amplicons out of total target bases submitted.
† Typical coverage uniformity—bases covered at >20% of the mean coverage.

For Research Use Only. Not for use in diagnostic procedures.