Extensive gene coverage with pre-designed inherited disease and cancer panels

Transformative target selection technology

Ion AmpliSeq™ ready-­to-­use NGS panels transform genetic research with ultrahigh-multiplex PCR primer pools designed for fast and simple production of thousands of targeted amplicons. The breakthrough low input DNA requirement of the Ion AmpliSeq panels enables the practical interrogation of research samples containing as little as 1 ng of input DNA, such as formalin-fixed, paraffin­embedded (FFPE) and fine needle biopsy (FNB) samples. In addition, using the Ion AmpliSeq™ Direct FFPE DNA Kit, you can prepare high-quality FFPE DNA in only 30 minutes with just 2 pipetting steps and less than 10 minutes hands-on time.

The convenient pre­designed panels allow researchers to focus on data generation and analysis, not on the labor intensive primer design and target selection steps. While hybridization based target selection methods require 7 to 72 hours to complete both target selection and library preparation, Ion AmpliSeq technology makes it possible to complete the entire process in as little as 3.5 hours using simple PCR reactions. Additionally, Ion AmpliSeq library preparation steps are automated using the Ion Chef™ System, thus reducing the library preparation hands-on time to just 15 minutes for processing 8 samples per run.

Demo Video: Ion AmpliSeq library preparation automation using Ion Chef System