Ion AmpliSeq HD technology is a new amplicon-based library preparation technology for Ion Torrent next-generation sequencing (NGS) that gives you the power to design your own panels and find variants with a low limit of detection—down to 0.1% for cell-free DNA samples. This revolutionary technology enables researchers to routinely get answers when ultrahigh sensitivity is required, such as when detecting low-frequency alleles in circulating tumor DNA or trace pathogenic microbial species in blood.
Combined with the Ion GeneStudio S5 sequencers, Ion AmpliSeq HD panels offer a flexible targeted-NGS solution for:
- Cancer research
- Infectious disease research
- Microbial studies
- Gene expression studies
How does Ion AmpliSeq HD technology work?
Watch the video below to learn more about this novel technology.
Unmatched sensitivity for your research
As genetic research rapidly evolves, the ability to confidently detect low-frequency variants or quantitate transcript-level expression is becoming more critical to the development of NGS assays with ultrahigh sensitivity. Ion AmpliSeq HD panels have been shown to achieve a 0.1% limit of detection for cell-free DNA samples, with >99% specificity for SNVs, hotspots, indels, CNVs, and fusions. Ion AmpliSeq HD panels support both DNA and RNA samples and require as little as 1 ng nucleic acids input from FFPE samples.
Rapid two-day targeted NGS workflow
- Library prep (Ion AmpliSeq HD Library Kit, Ion AmpliSeq HD Dual Barcode Kit)
- Automated template prep (Ion Chef System)
- Ion GeneStudio S5 Systems
- Torrent Suite Software
- Ion Reporter Software
- Oncomine Knowledgebase Software
For Research Use Only. Not for use in diagnostic procedures.