Working with RNA: The Basics | Thermo Fisher Scientific

Obtaining high quality, intact RNA is the first and often the most critical step in performing many molecular biology experiments. The Ambion team of RNA experts are dedicated to helping you get off to the right start with your RNA isolation. To that end we have written a number of technical articles addressing the RNA Basics found on this page as well as a library full of technical notes, tips and tricks videos, webinars and more in the Ambion Technical Resource page.

Get the basics on complex protocols

Designed to provide in-depth assistance for first-time users of our more complex products or protocols; check out The Basics series below:

The Basics: RNA Isolation
The Basics: RNase Control
The Basics: In Vitro Translation
In Vitro Transcription
The Basics: Northern Analysis
The Basics: Nuclease Protection Assays
The Basics: RT-PCR

Tips and tricks for RNA analysis

Tips for Handling RNA
Top Ten Ways to Improve Your RNA Isolation
Top Ten Most Common Real-Time qRT-PCR Pitfalls
Top Ten Pitfalls in Quantitative Real-Time PCR Primer Probe Design & Use
Ten Tips for In Situ Hybridization of Tissue Microarrays
Ten Ways to Increase the Sensitivity of Northern Hybridizations
Top Ten Northern Analysis Tips
Top Ten Tips for a Successful siRNA Experiment
Top Ten Ways to Optimize siRNA Delivery in Cultured Cells
Top Ten Ways to Ensure Valid RNAi Data

Resources

Find Products with the RNA/DNA Product Selection Guide
Review Ambion TechNotes Articles
Explore RNA Protocols

For Research Use Only. Not for use in diagnostic procedures.