Description: A protocol for 3' end labeling RNA using T4 RNA ligase and [32P]pCp.
Protocol for 5' End Labeling RNA
Description: This protocol is for 5' end labeling RNA with T4 polynucleotide kinase (PNK).
RNA Structure/Function Protocols: Alkaline Hydrolysis, RNA Sequencing and RNA Structure Analyses with Nucleases
Description: How to use RNases A, T1 and V1 to provide information about the structure of an RNA molecule. Includes two protocols for preparing RNA "ladders" for analysis by gel electrophoresis.
End Labeling RNA for Structural Analysis - TechNotes 9(4)
Description: Tips for radiolabeling your RNA on either the 5' or 3' end for subsequent nuclease digestion or footprinting analysis.
Products for RNA Structure/Function Analysis - TechNotes 8(5)
Description: Biochemical grade RNases and modified rNTPs for structural characterization of RNA