Explore our “Getting Started” and “Troubleshooting” sections for solutions to top inquiries and common problems. Browse through our "Guides and Tools" section to access a comprehensive portfolio of product-related support resources.
- I want to track my cells over time, and you have a lot of options to choose from. How do I pick the right one?
- I want to track my cells with a nucleic acid stain, like DAPI or Hoechst™ dye. Do you recommend this?
- I like how calcein dyes label the whole cell. How long can I track my cells with them, and can I fix them?
- What is degree of labeling (DOL)?
- What amount of conjugated antibody should I inject to image tumors?
- I’m trying to stain my cells with CellTracker™ dyes or CFDA SE, but I’m not seeing much signal. What can I do?
- When I label with Qtracker™ cell labeling reagents, I get a punctate label pattern. How do I make it more uniform?
- I stained my cells with a lipophilic cyanine dye, like DiI C18, but the signal was lost when I tried to follow up with antibody labeling. Why?
- I labeled my cells with Calcein, AM, but the signal was lost after I fixed my cells. Why is this?
- I labeled two populations of cells, one with CellTracker™ Green and the other with CellTracker™ Red, but it looks like there may be crossover of the red dye to the green cells. What is causing this?
Guides and Tools
- Overview: Cell Tracing, Tracking, and Morphology
- Overview: Small Animal In Vivo Imaging (SAIVI™)
- Overview: Molecular Probes™ School of Fluorescence
- Learning Center: Cell Analysis
- Cell Analysis Resource Library (useful technical, application, and other learning resources including application notes and methods)
- Guide: The Molecular Probes™ Handbook
- Images: Image Gallery
- Journal Articles: BioProbes Journal of Cell Biology Applications
- Newsletter: ProbesOnline™ Newsletter
- Protocols: Cell Analysis Protocols
- Tutorials: Fluorescence and Flow Cytometry Tutorials
- Fluorescence SpectraViewer : Plot and compare spectra, check spectral compatibility, and email the configuration to yourself or others in the lab.
- Cell Staining Simulation Tool: Create your perfectly labeled fluorescent cell and share it with your colleagues using the email or print function.
- Antibody Search Tool
- Fluorescence SpectraViewer Mobile App: An app to plot and compare spectra, check spectral compatibility, and email the configuration to yourself or others in the lab. This mobile version of SpectraViewer acts as an extension of our online Fluorescence SpectraViewer.
- Alexa Fluor™ Selection Guide Widget: A widget designed to provide you with a concise and informative set of criteria for choosing the optimal Alexa Fluor® dye for your antibody or protein labeling application.
- Cell Imaging Reagent Guide & Protocols App: An app designed to help you find fluorescent dyes, reagents, kits, and protocols for cell biology-related fluorescence microscopy applications. Also included are product guides for instrument calibration and setup, protein labeling and secondary detection.
- 3D Cell Simulation & Cell Staining Tool (for iPad™ device): Learn about the cell and all its structures, rotate the cell 360 degrees, watch live cell videos, and virtually stain your own cell with Molecular Probes™ dyes.
- Gibco™ Cell Culture Companion: An app designed to capture and store vital experiment information as you count cells, perform cell culture calculations and generate growth curves.
- DailyCalcs Science Calculator: An app designed to calculate molarity, dilution, molecular weight, and more. Version 3.0 now has eight calculators, including a new Specific Productivity Calculator for use with fed-batch cell culture processes and a Transfection calculator you can use to simplify transfection experimental setup.
- Flow Cytometry Reagent Guide and Protocols App: An app designed to help you find fluorescent reagents, kits, and protocols for cell biology–related flow cytometry applications. Also included are product guides for instrument calibration and setup, protein labeling, and secondary detection.
All the components necessary to develop the most appropriate assay for your research or discovery target of interest are available from us with our custom solutions. Additionally, our dedicated project managers will guide your project through every step of the process, and constantly keep you informed of our progress. Let us take the hassle out of your hands with a custom solution for you.
With a proven track record of over 18,000 custom antibody projects completed to date, our custom antibody development, purification, and screening services deliver the quality, consistency, and performance you need. Once your custom project is underway, you can track the real-time progress of your project via OpenProject™ software. Our manufacturing facilities are ISO 13485:2003 certified and registered with the FDA. We offer USDA, and OLAW accreditation standard, and AAALAC form accreditation by special request.
Every cell culture project is unique. We are committed to providing you with products and services customized to your research and production needs. Presenting the Gibco™ quality media you know and trust; customized to your specifications.
For Research Use Only. Not for use in diagnostic procedures.