Choosing a vector for the delivery of your gene

Illustration of the pcDNA 3.1 vector map

Selecting a vector is key to experimental design. Your vector choice is also important for us to know to build your construct quickly and efficiently. Once your gene is synthesized, there are several options for insertion into a vector to help provide you with the maximum flexibility and speed of delivery.

Please note: Cloning services are only offered for de novo genes synthesized by the GeneArt Services team.

Vector options for your synthesized gene:

Basic shuttle vector, pMX
Direct cloning vectors
A broad range of vectors, including Invitrogen vectors
Send us your personal vector

If you’d prefer to start with synthetic linear DNA, we offer affordable and high-quality Strings DNA fragments delivered fast and ready to clone yourself.

Receive a clone in our standard shuttle vector

Our standard shuttle vector, pMX, is a pUC19 derivative with a choice of antibiotic resistance markers. These vectors are intended for delivery of your clonal synthetic DNA. You simply excise your gene via restriction enzymes or PCR amplification and clone as you wish. Any specific flanking restriction enzymes you desire should be included in the gene sequence design provided for de novo synthesis.

Get your de novo gene cloned into our pMX vector using the GeneArt Dashboard

Receive a clone in a direct cloning vector

Illustration of the pcDNA 3.4-TOPO vector map

Save up to six days of production time with direct cloning into one of a growing list of popular vectors, such as pcDNA 3.4 to get your expression-ready genes faster. We offer a range of vectors for applications including Gateway cloning, recombinant antibody expression, and expression of proteins in mammalian, bacterial, insect, yeast, or cell-free systems.

Explore available vectors for direct cloning using our vector selection tool

To qualify for direct cloning, genes must be <5 kb and must not contain complex sequences. To optimize your gene sequence, reduce complexity, and maximize protein expression levels, use the Invitrogen GeneArt GeneOptimizer algorithm, available when designing clones in the GeneArt Dashboard.

Express delivery and SuperSPEED service saves additional production time if you need your clone fast. We offer many options that make it possible to obtain your cloned, expression-ready genes fast with the same high quality you rely on.

Use one of our popular Invitrogen vectors

We can clone your de novo gene into one of the many commercially available Invitrogen vectors that cover a multitude of downstream applications. If you don’t see a vector for selection from the drop-down in the classic ordering portal, you can purchase a commercially available Invitrogen vector and send it to us.

Gateway cloning offers unique benefits

Gateway is a proven and highly cited cloning system that is ideal to shuttle genes of interest between vectors, using site-specific recombination-based cloning. This system offers a simple method to determine an optimal expression system for your research. As the sole provider of the Gateway recombination cloning technology we can subclone your gene into one of our many Gateway vectors.

Gateway offers an easy-to use choice for cloning in multiple expression systems, the insert’s proper orientation and reading frame are maintained during shuttling using the Gateway vectors saving time and effort.

Explore all available vectors using our vector selection tool