Hypercarb HPLC and UHPLC Columns | Thermo Fisher Scientific

Your solution for difficult separations in both reversed phase and normal phase HPLC and LC/MS applications

Porous graphitic carbon (PGC, Hypercarb™) has unique properties as a stationary phase in high-performance liquid chromatography (HPLC). Its chemical surface properties distinguish it from more conventional LC packings such as bonded-silica gels and polymers. PGC behaves as a strongly retentive alkyl-bonded silica gel for non-polar analytes.

However, its retention and selectivity behavior towards polar and structurally-related compounds is very different from conventional C18 columns.

Features of Hypercarb columns

Thermo Scientific™ Hypercarb Porous Graphitic Carbon LC Columns offer:

Exceptional retention of very polar analytes
Separation for structurally-related substances
pH stablility from 0 to 14, and are not affected by aggressive mobile phases
Ideal solution for high-temperature applications

Physical properties of PGC

Property		To meet requirement of
Particle shape:	Spherical	No micropores
Specific surface area:	120 m²/g	Retention linearity and loading capacity
Median pore diameter:	250 Å	Mass transfer for wide range of analyte shapes and sizes
Pore volume:	0.7 m³/g
Mean particle diameters:	3 µm, 5 µm	Analytical HPLC columns
	7 µm	Preparative HPLC columns
	30 µm	SPE applications
Porosity:	75%	Mass transfer within particles
% C:	100%	Chemical stability
Mechanical strength:	<400="">	Operational particle stability; pressure gradients in packing process

Hypercarb columns literature

2022/2023 HPLC/UHPLC Columns Catalog

Access the latest interactive version of our Chromatography Consumables & Columns Catalog. Our enhanced online version features easier access to product information, key application notes and brand-new selector tools.

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