Do you want to separate ionic and hydrophobic analytes in a single run? Separate polar, nonpolar, and ionizable compounds in a single analysis and achieve baseline separation of challenging polar analytes without derivatizing using a mixed-mode HPLC column. The stationary phases of Thermo Scientific mixed-mode columns combine reversed phase, ion-exchange, and/or HILIC selectivity. This eliminates the need for column switching, ion-paring, and using columns in series. Easily optimize selectivity by adjusting mobile phase buffer concentration, pH, and solvent content to improve chromatographic separations and increase productivity.
Mixed-mode HPLC columns are well-suited for retaining ionic analytes, including pharmaceutical drugs and their counter ions. Mixed-mode liquid chromatography does not require the use of ion-pair reagents in the mobile phase, significantly improving mass spectrometry (MS) compatibility.
Acclaim Trinity P1 HPLC ColumnsCation-exchange, anion-exchange, and reversed phase selectivity
Acclaim Trinity P2 HPLC ColumnsCation-exchange, anion-exchange, and
Acclaim Trinity Q1 HPLC ColumnsCation-exchange, anion-exchange, and reversed phase selectivity
Acclaim Mixed-Mode WAX-1 HPLC ColumnsReversed phase and anion-exchange selectivity
Acclaim Mixed-Mode WCX-1 HPLC ColumnsReversed phase and cation-exchange selectivity
Acclaim Mixed-Mode HILIC-1 HPLC ColumnsReversed phase and HILIC selectivity
Both our Thermo Scientific Acclaim Trinity column line and Thermo Scientific Acclaim Mixed-Mode column line are compatible with the Thermo Scientific Acclaim guard holder and coupler.
Discover the latest in mixed-mode liquid chromatography technology, learn about the unique chromatographic properties of mixed-mode columns, and explore analytical challenges that have been addressed by mixed-mode liquid chromatography in this webinar led by Dr. Joachim P. Weiss, Ph.D. and Serdar Bilgesoy.
Acclaim Trinity P1 mixed-mode chromatography column coupled with an inert LC system: an innovative approach for underivatized highly polar pesticides separation.
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