Low Flow HPLC Columns | Thermo Fisher Scientific

Overview

Enhanced performance of low-flow LC-MS and HPLC separations

Low-flow chromatography is ideal when detailed sample information is required from small sample volumes, especially for bottom-up and top-down proteomics research. Compared with analytical flow LC-MS (>100 µL/min), the signal with low-flow (<100 µL/min) can be many times higher. Low-flow columns—also known as micro, nano, and capillary columns—have a smaller diameter compared to analytical columns.

Thermo Scientific nano-, capillary-, and micro-flow columns offer excellent sensitivity and resolution in easy-to-use formats:

Thermo Scientific EASY-Spray HPLC Columns
Thermo Scientific double nanoViper HPLC Columns
Thermo Scientific μPAC Neo HPLC Columns

All HPLC and UHPLC columns

Low-flow (U)HPLC Columns Connectivity Whiteboard Video

Learn more about the power of Thermo Scientific low-flow columns and discover column designs that ensure near zero-dead volume connections to give you confidence in your data quality. Thermo Scientific low-flow (U)HPLC columns — technology that’s beyond brilliant.

Low-flow columns for reliable and robust data

Low-flow (U)HPLC column formats
EASY-Spray (U)HPLC Columns	Double nanoViper (U)HPLC Columns	µPAC Neo HPLC Columns

Click-and-spray Connection with EASY-Spray MS source nanoViper connection Integrated column and emitter Integrated temperature control For use with Thermo Scientific MS systems	Universal Thermo Scientific nanoViper fingertight fittings for column inlet and outlet nanoViper connection Separate emitters Compatible with all low-flow U/HPLC instruments	Excellent retention time stability Low back pressure separations, improving column and emitter robustness High flexibility Compatible with all low-flow U/HPLC instruments nanoViper connection

EASY-Spray (U)HPLC columns

Choose an EASY-Spray column when:	What makes an EASY-Spray column special?

You want simple connections with an EASY-Spray source. This is ideal for novice users. Sample amount is limited Analytical UHPLC does not provide sufficient sensitivity Workflow simplicity is key High sensitivity is required to identify proteins and peptides at low expression levels Analyses are done in a targeted and untargeted way for screening and verification	Unique design provides uncompromised performance in an ease of use format for nano and capillary LC-MS analysis. Features for optimum data quality: Simple connection to the LC and Thermo Scientific MS instruments Precision machined and positioned glass emitters Integrated nanoViper zero-dead-volume (ZDV) unions Integrated temperature control

Recommended EASY-Spray UHPLC columns by application

EASY-Spray UHPLC columns for bottom-up proteomics

EASY-Spray PepMap Neo UHPLC columns

The Thermo Scientific EASY-Spray PepMap Neo UHPLC columns are perfect for bottom-up proteomics. Packed at higher pressure and rated to 1500 bar, they provide consistent column-to-column performance, long column lifetime, and excellent efficiency. These benefits are true at any pressure.

Catalog number	Product line	Particle size (µm)	Column ID (µm)	Column length (mm)	Order
ES75150PN	PepMap Neo columns	2	75	150	Order now ›
ES75500PN	PepMap Neo columns	2	75	500	Order now ›
ES75750PN	PepMap Neo columns	2	75	750	Order now ›

Also see: EASY-Spray PepMap HPLC columns

EASY-Spray columns for top-down proteomics

EASY-Spray MAbPac RP columns

The Thermo Scientific MAbPac RP capillary column is best suited for the characterization of intact proteins in top-down proteomics, clinical and anti-doping applications where sample amount is limited, or sensitivity is crucial.

Catalog number	Product line	Particle size (µm)	Column length (mm)	Column ID (µm)	Order
ES907	MAbPac RP column	4	150	150	Order now ›

Traps and accessories

For the best performance from your EASY-Spray column, consider investing in the following accessories.

Traps

Catalog number	Product line	Particle size (µm)	Column ID (µm)	Media bed length (mm)	Trap length (mm)	Union type	Order
174500	PepMap Neo Trap Cartridge 3PK	5	300	5	N/A	N/A	Order now ›
164199	PepMap nanotrap 500 bar 2PK	5	100	20	150	Nut/sleeve	Order now ›
164750	PepMap nanotrap 500 bar 2PK	5	100	20	150	Double nanoViper	Order now ›
164535	PepMap nanotrap 500 bar 2PK	3	75	20	150	Double nanoViper	Order now ›
164946	PepMap nanotrap 1200 bar 2PK	3	75	20	70	Double nanoViper	Order now ›
164213	PepMap nanotrap 500 bar 2PK	5	200	20	150	Nut/sleeve	Order now ›

PEEK tubing and trap holder

Catalog number	Product line	For use with	Order
174501	PEEK with nanoViper fittings 30 µm X100 mm 2PK 1500 bar	Low-flow PepMap columns	Order now ›
174502	Trap holder + nanoViper fittings kit 1500 bar	Low-flow PepMap columns	Order now ›

Double nanoViper (U)HPLC columns


Choose these columns when:	What makes these columns special?
Maximum flexibility is required Changing the emitter and column independently is important	These stand-alone nano-, capillary, and micro-flow columns are: Designed with single nanoViper and double nanoViper fingertight fittings for trouble-free connection For robust separation in proteomics research, drug discovery, and high-throughput proteomics laboratories

Choose these columns when:

What makes these columns special?

Maximum flexibility is required
Changing the emitter and column independently is important

These stand-alone nano-, capillary, and micro-flow columns are:

Designed with single nanoViper and double nanoViper fingertight fittings for trouble-free connection
For robust separation in proteomics research, drug discovery, and high-throughput proteomics laboratories

Recommended nano- and capillary HPLC columns by application

Nano-/Capillary UHPLC columns for bottom-up proteomics

Double nanoViper PepMap Neo UHPLC columns

Use these columns for peptide bottom-up proteomics studies. They are packed at higher pressure and rated a 1500 bar and provide consistent column-to-column performance, long column lifetime, and excellent efficiency. These benefits are true at any pressure.

Catalog number	Product line	Particle size (µm)	Column ID (µm)	Column length (mm)	Order
DNV75150PN	PepMap Neo column	2	75	150	Order now ›
DNV75500PN	PepMap Neo column	2	75	500	Order now ›
DNV75750PN	PepMap Neo column	2	75	750	Order now ›

Also available: Single nanoViper PepMap C18 HPLC columns

Nano-/Capillary UHPLC columns for top-down proteomics

MAbPac capillary reversed phase (RP) HPLC column

The Thermo Scientific MAbPac RP capillary column is best suited for the characterization of intact proteins in top-down proteomics, clinical, and anti-doping applications where sample amount is limited, or sensitivity is crucial.

Catalog number	Product line	Particle size (µm)	Column ID (µm)	Column length (mm)	Order
164947	MAbPac RP column	4	150	150	Order now ›

Nano-/Capillary UHPLC columns for free glycans

Accucore 150 Amide HILIC HPLC column

Separate hydrophilic biomolecules in HILIC mode
Excellent for released glycan separations

Catalog number	Product line	Particle size (µm)	Column ID (µm)	Column length (mm)	Order
16726-157569	Amide HILIC column	2.6	75	150	Order now ›

LC-MS connection accessories and emitters

These emitters, nanoViper tubing kits, and unions offer easy connection from your LC system to an EASY-Spray source.

Catalog number	Product line	For use with	Order
6040.2304	Two Viper unions	Double nanoViper columns	Order now ›
6041.5260	nanoViper tubing 20 µm x 550 mm	Double nanoViper columns	Order now ›
ES993	Emitter: 10 µm I.D.	Double nanoViper columns	Order now ›
ES994	Emitter: 15 µm I.D	Double nanoViper columns	Order now ›

Traps and accessories

For the best performance from your double nanoViper column consider investing in the following accessories.

Catalog number	Product line	Particle size (µm)	Column ID (µm)	Media bed length (mm)	Trap length (mm)	Union type	Order
174500	PepMap Neo Trap Cartridge 3PK	5	300	5	N/A	N/A	Order now ›
164199	PepMap nanotrap 500 bar 2PK	5	100	20	150	Nut/sleeve	Order now ›
164750	PepMap nanotrap 500 bar 2PK	5	100	20	150	Double nanoViper	Order now ›
164535	PepMap nanotrap 500 bar 2PK	3	75	20	150	Double nanoViper	Order now ›
164946	PepMap nanotrap 1200 bar 2PK	3	75	20	70	Double nanoViper	Order now ›
164213	PepMap nanotrap 500 bar 2PK	5	200	20	150	Nut/sleeve	Order now ›

Catalog number	Product line	For use with	Order
174501	PEEK with nanoViper fittings 30 µm X100 mm 2PK 1500 bar	Low-flow PepMap Neo columns	Order now ›
174502	Trap holder + nanoViper fittings kit 1500 bar	Low-flow PepMap Neo columns	Order now ›

μPAC Neo HPLC columns


Choose a μPAC Neo HPLC column when:	What makes µPAC Neo HPLC columns special?
Highest resolution and peak capacities is required Your samples span a wide concentration range Highest LC-MS sensitivity is needed You want to speed up your runtimes LC-MS robustness is needed You want an increased column lifetime You prefer working at much lower back pressures than with packed-bed columns When it is important to compare results from experiments spanning over time or geographical location	The unique separation path provides: µ-pillar stationary backbone, micromachined in a silicon wafer Flow path designed for highest analyte concentration during elution Extra-high resolution separations, using up to 110 cm column lengths Unique combination of performance and reliability for high sample coverage every time

Choose a μPAC Neo HPLC column when:

What makes µPAC Neo HPLC columns special?

Highest resolution and peak capacities is required
Your samples span a wide concentration range
Highest LC-MS sensitivity is needed
You want to speed up your runtimes
LC-MS robustness is needed
You want an increased column lifetime
You prefer working at much lower back pressures than with packed-bed columns
When it is important to compare results from experiments spanning over time or geographical location

The unique separation path provides:

µ-pillar stationary backbone, micromachined in a silicon wafer
Flow path designed for highest analyte concentration during elution
Extra-high resolution separations, using up to 110 cm column lengths
Unique combination of performance and reliability for high sample coverage every time

μPAC Neo HPLC columns

These HPLC columns are ideally suited for bottom-up proteomics where resolution is critical to the success of the analysis. Our µPAC Neo columns provide excellent column-to-column reproducibility and peak capacity at a wide range of flow rates and sample amounts.

Catalogue number	Product line	Column length (cm)	Flow rate range (nL/min)	Sample loading	Gradient time	Order
COL-NANO050NEOB	µPAC Neo	50	100-750	< 1 μg	15 – 120 min	Order now ›
COL-NANO110NEOB	µPAC Neo	110	100-750	< 4 μg	> 90 min	Order now ›
COL-LOLO050NEOB	µPAC Neo low-load	50	100-750	< 10 ng	15 – 90 min	Order now ›

μPAC Neo trapping columns

Catalogue number	Product line	For use with	Flow rate range (μL/min)	Sample loading	Order
COL-TRPLOLONEOB2 (Duo-Pack)	μPAC Neo low-load trapping column	μPAC Neo low-load HPLC column	100-750	< 10 ng	Order now ›

μPAC Neo accessories

Catalogue number	Product line	For use with	Order
6040.2304	Union for Viper and nanoViper Tubing	Double nanoViper and μPAC Neo Columns	Order now ›
6041.5260	nanoViper tubing 20 μm x 550 mm	Double nanoViper and μPAC Neo Columns	Order now ›
ES993	Nano EASY-Spray emitter, 10 μm I.D.	Double nanoViper and μPAC Neo Columns	Order now ›

µPAC HPLC columns

These HPLC columns are ideally suited for bottom-up proteomics where resolution is critical to the success of the analysis. Our µPAC HPLC columns provide excellent column-to-column reproducibility and peak capacity at a wide range of flow rates.

Catalogue number	Product line	Pillar diameter (µm)	Interpillar distance (µm)	Column length (cm)	Flow rate range (µL/min)	Order
COL-nano050G1B	µPAC pillar array	5	2.5	50	0.1 – 2.0	Order now ›
COL-nano200G1B	µPAC pillar array	5	2.5	200	0.1 - 1.0	Order now ›
COL-cap050G1B	µPAC pillar array (capillary flow)	5	2.5	50	1.0 - 15.0	Order now ›

µPAC traps

Catalogue number	Product line	Pillar diameter (µm)	Interpillar distance (µm)	Column length (cm)	Order
COL-trpnano16G1B2 (Duo-Pack)	µPAC pillar array	5	2.5	1	Order now ›

µPAC accessories

Catalogue number	Product line	For use with	Order
EMI-flexionB	μPAC Flex iON Connect ESI-MS: Interface to connect μPAC nanoLC columns to a Nanospray Flex Series ion source	μPAC nano columns	Order now ›
EMI-easysprayB	μPAC Compatible EASY-Spray Emitter: Pre-assembled EASY-Spray emitter, grounding union, and transfer line to connect μPAC nanoLC columns to an EASY-Spray source	μPAC nano columns	Order now ›