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Overview
Enhanced performance of low-flow LC-MS and HPLC separations
Low-flow chromatography is ideal when detailed sample information is required from small sample volumes, especially for bottom-up and top-down proteomics research. Compared with analytical flow LC-MS (>100 µL/min), the signal with low-flow (<100 µL/min) can be many times higher. Low-flow columns—also known as micro, nano, and capillary columns—have a smaller diameter compared to analytical columns.
Thermo Scientific nano-, capillary-, and micro-flow columns offer excellent sensitivity and resolution in easy-to-use formats:
- Thermo Scientific EASY-Spray HPLC Columns
- Thermo Scientific double nanoViper HPLC Columns
- Thermo Scientific μPAC Neo HPLC Columns
All HPLC and UHPLC columns
- Popular small molecule HPLC and UHPLC column brands
- Biomolecule (U)HPLC columns
- Reversed phase (U)HPLC columns
- HILIC (U)HPLC columns
- Mixed-mode HPLC columns
- Application specific HPLC columns
- Normal phase HPLC columns
- Ion exclusion HPLC columns
- Preparative HPLC columns
- HPLC guard columns, cartridges, and accessories
Low-flow (U)HPLC Columns Connectivity Whiteboard Video
Learn more about the power of Thermo Scientific low-flow columns and discover column designs that ensure near zero-dead volume connections to give you confidence in your data quality. Thermo Scientific low-flow (U)HPLC columns — technology that’s beyond brilliant.
Low-flow columns for reliable and robust data
Low-flow (U)HPLC column formats | ||
| EASY-Spray (U)HPLC Columns | Double nanoViper (U)HPLC Columns | µPAC Neo HPLC Columns |
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Click-and-spray Connection with EASY-Spray MS source
EASY-Spray (U)HPLC columns
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| Choose an EASY-Spray column when: | What makes an EASY-Spray column special? |
|---|---|
| Unique design provides uncompromised performance in an ease of use format for nano and capillary LC-MS analysis. Features for optimum data quality:
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Recommended EASY-Spray UHPLC columns by application
EASY-Spray PepMap Neo UHPLC columns
The Thermo Scientific EASY-Spray PepMap Neo UHPLC columns are perfect for bottom-up proteomics. Packed at higher pressure and rated to 1500 bar, they provide consistent column-to-column performance, long column lifetime, and excellent efficiency. These benefits are true at any pressure.
| Catalog number | Product line | Particle size (µm) | Column ID (µm) | Column length (mm) | Order |
|---|---|---|---|---|---|
| ES75150PN | PepMap Neo columns | 2 | 75 | 150 | Order now › |
| ES75500PN | PepMap Neo columns | 2 | 75 | 500 | Order now › |
| ES75750PN | PepMap Neo columns | 2 | 75 | 750 | Order now › |
Also see: EASY-Spray PepMap HPLC columns
EASY-Spray MAbPac RP columns
The Thermo Scientific MAbPac RP capillary column is best suited for the characterization of intact proteins in top-down proteomics, clinical and anti-doping applications where sample amount is limited, or sensitivity is crucial.
| Catalog number | Product line | Particle size (µm) | Column length (mm) | Column ID (µm) | Order |
|---|---|---|---|---|---|
| ES907 | MAbPac RP column | 4 | 150 | 150 | Order now › |
Traps and accessories
For the best performance from your EASY-Spray column, consider investing in the following accessories.
Traps
| Catalog number | Product line | Particle size (µm) | Column ID (µm) | Media bed length (mm) | Trap length (mm) | Union type | Order |
|---|---|---|---|---|---|---|---|
| 174500 | PepMap Neo Trap Cartridge 3PK | 5 | 300 | 5 | N/A | N/A | Order now › |
| 164199 | PepMap nanotrap 500 bar 2PK | 5 | 100 | 20 | 150 | Nut/sleeve | Order now › |
| 164750 | PepMap nanotrap 500 bar 2PK | 5 | 100 | 20 | 150 | Double nanoViper | Order now › |
| 164535 | PepMap nanotrap 500 bar 2PK | 3 | 75 | 20 | 150 | Double nanoViper | Order now › |
| 164946 | PepMap nanotrap 1200 bar 2PK | 3 | 75 | 20 | 70 | Double nanoViper | Order now › |
| 164213 | PepMap nanotrap 500 bar 2PK | 5 | 200 | 20 | 150 | Nut/sleeve | Order now › |
PEEK tubing and trap holder
| Catalog number | Product line | For use with | Order |
|---|---|---|---|
| 174501 | PEEK with nanoViper fittings 30 µm X100 mm 2PK 1500 bar | Low-flow PepMap columns | Order now › |
| 174502 | Trap holder + nanoViper fittings kit 1500 bar | Low-flow PepMap columns | Order now › |
Double nanoViper (U)HPLC columns
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| Choose these columns when: | What makes these columns special? |
|---|---|
| These stand-alone nano-, capillary, and micro-flow columns are:
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Recommended nano- and capillary HPLC columns by application
Double nanoViper PepMap Neo UHPLC columns
Use these columns for peptide bottom-up proteomics studies. They are packed at higher pressure and rated a 1500 bar and provide consistent column-to-column performance, long column lifetime, and excellent efficiency. These benefits are true at any pressure.
| Catalog number | Product line | Particle size (µm) | Column ID (µm) | Column length (mm) | Order |
|---|---|---|---|---|---|
| DNV75150PN | PepMap Neo column | 2 | 75 | 150 | Order now › |
| DNV75500PN | PepMap Neo column | 2 | 75 | 500 | Order now › |
| DNV75750PN | PepMap Neo column | 2 | 75 | 750 | Order now › |
Also available: Single nanoViper PepMap C18 HPLC columns
MAbPac capillary reversed phase (RP) HPLC column
The Thermo Scientific MAbPac RP capillary column is best suited for the characterization of intact proteins in top-down proteomics, clinical, and anti-doping applications where sample amount is limited, or sensitivity is crucial.
| Catalog number | Product line | Particle size (µm) | Column ID (µm) | Column length (mm) | Order |
|---|---|---|---|---|---|
| 164947 | MAbPac RP column | 4 | 150 | 150 | Order now › |
Accucore 150 Amide HILIC HPLC column
- Separate hydrophilic biomolecules in HILIC mode
- Excellent for released glycan separations
| Catalog number | Product line | Particle size (µm) | Column ID (µm) | Column length (mm) | Order |
|---|---|---|---|---|---|
| 16726-157569 | Amide HILIC column | 2.6 | 75 | 150 | Order now › |
These emitters, nanoViper tubing kits, and unions offer easy connection from your LC system to an EASY-Spray source.
| Catalog number | Product line | For use with | Order |
|---|---|---|---|
| 6040.2304 | Two Viper unions | Double nanoViper columns | Order now › |
| 6041.5260 | nanoViper tubing 20 µm x 550 mm | Double nanoViper columns | Order now › |
| ES993 | Emitter: 10 µm I.D. | Double nanoViper columns | Order now › |
| ES994 | Emitter: 15 µm I.D | Double nanoViper columns | Order now › |
For the best performance from your double nanoViper column consider investing in the following accessories.
| Catalog number | Product line | Particle size (µm) | Column ID (µm) | Media bed length (mm) | Trap length (mm) | Union type | Order |
|---|---|---|---|---|---|---|---|
| 174500 | PepMap Neo Trap Cartridge 3PK | 5 | 300 | 5 | N/A | N/A | Order now › |
| 164199 | PepMap nanotrap 500 bar 2PK | 5 | 100 | 20 | 150 | Nut/sleeve | Order now › |
| 164750 | PepMap nanotrap 500 bar 2PK | 5 | 100 | 20 | 150 | Double nanoViper | Order now › |
| 164535 | PepMap nanotrap 500 bar 2PK | 3 | 75 | 20 | 150 | Double nanoViper | Order now › |
| 164946 | PepMap nanotrap 1200 bar 2PK | 3 | 75 | 20 | 70 | Double nanoViper | Order now › |
| 164213 | PepMap nanotrap 500 bar 2PK | 5 | 200 | 20 | 150 | Nut/sleeve | Order now › |
| Catalog number | Product line | For use with | Order |
|---|---|---|---|
| 174501 | PEEK with nanoViper fittings 30 µm X100 mm 2PK 1500 bar | Low-flow PepMap Neo columns | Order now › |
| 174502 | Trap holder + nanoViper fittings kit 1500 bar | Low-flow PepMap Neo columns | Order now › |
μPAC Neo HPLC columns
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| Choose a μPAC Neo HPLC column when: | What makes µPAC Neo HPLC columns special? |
|---|---|
| The unique separation path provides:
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These HPLC columns are ideally suited for bottom-up proteomics where resolution is critical to the success of the analysis. Our µPAC Neo columns provide excellent column-to-column reproducibility and peak capacity at a wide range of flow rates and sample amounts.
| Catalogue number | Product line | Column length (cm) | Flow rate range (nL/min) | Sample loading | Gradient time | Order |
|---|---|---|---|---|---|---|
| COL-NANO050NEOB | µPAC Neo | 50 | 100-750 | < 1 μg | 15 – 120 min | Order now › |
| COL-NANO110NEOB | µPAC Neo | 110 | 100-750 | < 4 μg | > 90 min | Order now › |
| COL-LOLO050NEOB | µPAC Neo low-load | 50 | 100-750 | < 10 ng | 15 – 90 min | Order now › |
| Catalogue number | Product line | For use with | Flow rate range (μL/min) | Sample loading | Order |
|---|---|---|---|---|---|
| COL-TRPLOLONEOB2 (Duo-Pack) | μPAC Neo low-load trapping column | μPAC Neo low-load HPLC column | 100-750 | < 10 ng | Order now › |
| Catalogue number | Product line | For use with | Order |
|---|---|---|---|
| 6040.2304 | Union for Viper and nanoViper Tubing | Double nanoViper and μPAC Neo Columns | Order now › |
| 6041.5260 | nanoViper tubing 20 μm x 550 mm | Double nanoViper and μPAC Neo Columns | Order now › |
| ES993 | Nano EASY-Spray emitter, 10 μm I.D. | Double nanoViper and μPAC Neo Columns | Order now › |
These HPLC columns are ideally suited for bottom-up proteomics where resolution is critical to the success of the analysis. Our µPAC HPLC columns provide excellent column-to-column reproducibility and peak capacity at a wide range of flow rates.
| Catalogue number | Product line | Pillar diameter (µm) | Interpillar distance (µm) | Column length (cm) | Flow rate range (µL/min) | Order |
|---|---|---|---|---|---|---|
| COL-nano050G1B | µPAC pillar array | 5 | 2.5 | 50 | 0.1 – 2.0 | Order now › |
| COL-nano200G1B | µPAC pillar array | 5 | 2.5 | 200 | 0.1 - 1.0 | Order now › |
| COL-cap050G1B | µPAC pillar array (capillary flow) | 5 | 2.5 | 50 | 1.0 - 15.0 | Order now › |
| Catalogue number | Product line | Pillar diameter (µm) | Interpillar distance (µm) | Column length (cm) | Order |
|---|---|---|---|---|---|
| COL-trpnano16G1B2 (Duo-Pack) | µPAC pillar array | 5 | 2.5 | 1 | Order now › |
| Catalogue number | Product line | For use with | Order |
|---|---|---|---|
| EMI-flexionB | μPAC Flex iON Connect ESI-MS: Interface to connect μPAC nanoLC columns to a Nanospray Flex Series ion source | μPAC nano columns | Order now › |
| EMI-easysprayB | μPAC Compatible EASY-Spray Emitter: Pre-assembled EASY-Spray emitter, grounding union, and transfer line to connect μPAC nanoLC columns to an EASY-Spray source | μPAC nano columns | Order now › |
Thermo Scientific Vanquish Neo UHPLC System
The all-in-one nano-, capillary-, and micro-flow LC system for high sensitivity LCMS workflows.
Low-flow HPLC column videos
Webinar: Workflows for robust and sensitive separation of mAbs, intact proteins at subunit and peptide level by LC-MS
Biognosys AG: MAbPac RP 4 μm stand-alone and EASY-Spray LC columns and nano- and capillary-flow emitters
Technical University of Denmark (DTU): MAbPac RP 4 μm stand-alone and EASY-Spray LC columns and nano- and capillary-flow emitters
Related products
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Low-flow Columns Catalog
SureSTART Autosampler vials and caps
Velocity LFQ Data Independent Acquisition (DIA) Platform
Low-flow chromatography consumables reference guide for LC-MS proteomics research
Download this guide and learn more about the portfolio of Thermo Scientific consumables for LC-MS proteomics research and the value they can bring to your lab.
Resources for low-flow HPLC columns
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