Enhanced performance of low-flow LC-MS and HPLC separations

Low-flow chromatography is ideal when detailed sample information is required from small sample volumes, especially for bottom-up and top-down proteomics research. Compared with analytical flow LC-MS (>100 µL/min), the signal with low-flow (<100 µL/min) can be many times higher. Low-flow columns—also known as micro, nano, and capillary columns—have a smaller diameter compared to analytical columns.

Thermo Scientific nano-, capillary-, and micro-flow columns offer excellent sensitivity and resolution in easy-to-use formats:

  • Thermo Scientific EASY-Spray HPLC Columns
  • Thermo Scientific double nanoViper HPLC Columns
  • Thermo Scientific µPAC HPLC Columns

Learn more about the power of low-flow columns from Thermo Fisher Scientific — technology that’s beyond brilliant.

Low-flow columns for reliable and robust data

Low-flow HPLC column formats

EASY-Spray HPLC ColumnsDouble nanoViper HPLC ColumnsµPAC HPLC Columns
EASY-Spray HPLC Columns
Double nanoViper HPLC Columns
µPAC HPLC Columns
  • Click-and-spray Connection with EASY-Spray MS source
  • nanoViper connection
  • Integrated column and emitter
  • Integrated temperature control
  • For use with Thermo Scientific MS systems
  • Universal Thermo Scientific nanoViper fingertight fittings for column inlet and outlet
  • Simple zero-dead-volume (ZDV) connections
  • Separate emitters
  • Compatible with all low-flow U/HPLC instruments
  • Excellent retention time stability
  • Low back pressure separations, improving column and emitter robustness
  • Separate emitters
  • Compatible with all low-flow U/HPLC instruments
EASY-Spray columns
Choose an EASY-Spray column when:What makes an EASY-Spray column special?
  • You want simple connections with an EASY-Spray source. This is ideal for novice users.
  • Sample amount is limited
  • Analytical UHPLC does not provide sufficient sensitivity
  • Workflow simplicity is key
  • High sensitivity is required to identify proteins and peptides at low expression levels
  • Analyses are done in a targeted and untargeted way for screening and verification
Unique design provides uncompromised performance in an ease of use format for nano and capillary LC-MS analysis.
Features for optimum data quality:
  • Simple connection to the LC and Thermo Scientific MS instruments
  • Precision machined and positioned glass emitters
  • Integrated nanoViper zero-dead-volume (ZDV) unions
  • Integrated temperature control

Recommended EASY-Spray UHPLC columns by application

EASY-Spray MAbPac RP columns

The Thermo Scientific MAbPac RP capillary column is best suited for the characterization of intact proteins in top-down proteomics, clinical and anti-doping applications where sample amount is limited, or sensitivity is crucial.

Catalog numberProduct lineParticle size (µm)Column length (mm)Column ID (µm)Order
ES907MAbPac RP column4150150Order now ›

Traps and accessories

For the best performance from your EASY-Spray column, consider investing in the following accessories.

Traps

Catalog numberProduct lineParticle size (µm)Column ID (µm)Media bed length (mm)Trap length (mm)Union typeOrder
174500PepMap Neo Trap Cartridge 3PK53005N/AN/AOrder now ›
164199PepMap nanotrap 500 bar 2PK510020150Nut/sleeveOrder now ›
164750PepMap nanotrap 500 bar 2PK510020150Double nanoViperOrder now ›
164535PepMap nanotrap 500 bar 2PK37520150Double nanoViperOrder now ›
164946PepMap nanotrap 1200 bar 2PK3752070Double nanoViperOrder now ›
164213PepMap nanotrap 500 bar 2PK520020150Nut/sleeveOrder now ›

PEEK tubing and trap holder

Catalog numberProduct lineFor use withOrder
174501PEEK with nanoViper fittings 30 µm X100 mm 2PK 1500 barLow-flow PepMap columnsOrder now ›
174502Trap holder + nanoViper fittings kit 1500 barLow-flow PepMap columnsOrder now ›
Double nanoViper columns
Choose these columns when:What makes these columns special?
  • Maximum flexibility is required
  • Changing the emitter and column independently is important

These stand-alone nano-, capillary, and micro-flow columns are:

  • Designed with single nanoViper and double nanoViper fingertight fittings for trouble-free connection
  • For robust separation in proteomics research, drug discovery, and high-throughput proteomics laboratories

Recommended nano- and capillary HPLC columns by application

Double nanoViper PepMap Neo UHPLC columns

Use these columns for peptide bottom-up proteomics studies. They are packed at higher pressure and rated a 1500 bar and provide consistent column-to-column performance, long column lifetime, and excellent efficiency. These benefits are true at any pressure.

Catalog numberProduct lineParticle size (µm)Column ID (µm)Column length (mm)Order
DNV75150PNPepMap Neo column275150Order now ›
DNV75500PNPepMap Neo column275500Order now ›
DNV75750PNPepMap Neo column275750Order now ›

Also available: Single nanoViper PepMap C18 HPLC columns

MAbPac capillary reversed phase (RP) HPLC column

The Thermo Scientific MAbPac RP capillary column is best suited for the characterization of intact proteins in top-down proteomics, clinical, and anti-doping applications where sample amount is limited, or sensitivity is crucial.

Catalog numberProduct lineParticle size (µm)Column ID (µm)Column length (mm)Order
164947MAbPac RP column4150150Order now ›

Accucore 150 Amide HILIC HPLC column

  • Separate hydrophilic biomolecules in HILIC mode
  • Excellent for released glycan separations
Catalog numberProduct lineParticle size (µm)Column ID (µm)Column length (mm)Order
16726-157569Amide HILIC column2.675150Order now ›

These emitters, nanoViper tubing kits, and unions offer easy connection from your LC system to an EASY-Spray source.

Catalog numberProduct lineFor use withOrder
6040.2304Two Viper unionsDouble nanoViper columnsOrder now ›
6041.5260nanoViper tubing 20 µm x 550 mmDouble nanoViper columnsOrder now ›
ES993Emitter: 10 µm I.D.Double nanoViper columnsOrder now ›
ES994Emitter: 15 µm I.DDouble nanoViper columnsOrder now ›

For the best performance from your double nanoViper column consider investing in the following accessories.

Catalog numberProduct lineParticle size (µm)Column ID (µm)Media bed length (mm)Trap length (mm)Union typeOrder
174500PepMap Neo Trap Cartridge 3PK53005N/AN/AOrder now ›
164199PepMap nanotrap 500 bar 2PK510020150Nut/sleeveOrder now ›
164750PepMap nanotrap 500 bar 2PK510020150Double nanoViperOrder now ›
164535PepMap nanotrap 500 bar 2PK37520150Double nanoViperOrder now ›
164946PepMap nanotrap 1200 bar 2PK3752070Double nanoViperOrder now ›
164213PepMap nanotrap 500 bar 2PK520020150Nut/sleeveOrder now ›
Catalog numberProduct lineFor use withOrder
174501PEEK with nanoViper fittings 30 µm X100 mm 2PK 1500 barLow-flow PepMap Neo columnsOrder now ›
174502Trap holder + nanoViper fittings kit 1500 barLow-flow PepMap Neo columnsOrder now ›
µPAC HPLC columns
Choose a µPAC HPLC column when:What makes µPAC HPLC columns special?
  • Highest resolution and peak capacities is required
  • Your samples span a wide concentration range
  • Highest LC-MS sensitivity is needed
  • You want to speed up your runtimes
  • LC-MS robustness is needed
  • You want an increased column lifetime
  • You prefer working at much lower back pressures than with packed-bed columns
  • When it is important to compare results from experiments spanning over time or geographical location
The unique separation path provides:
  • µ-pillar stationary backbone, micromachined in a silicon wafer
  • Flow path designed for highest analyte concentration during elution
  • Extra-high resolution separations, using up to 200 cm column lengths
  • Low back pressure separations, improving column and emitter robustness

These HPLC columns are ideally suited for bottom-up proteomics where resolution is critical to the success of the analysis. Our µPAC HPLC columns provide excellent column-to-column reproducibility and peak capacity at a wide range of flow rates.

Catalogue numberProduct linePillar diameter (µm)Interpillar distance (µm)Column length (cm)Flow rate range (µL/min)Order
COL-nano050G1BµPAC pillar array52.5500.1 – 2.0Order now ›
COL-nano200G1BµPAC pillar array52.52000.1 - 1.0Order now ›
COL-cap050G1BµPAC pillar array (capillary flow)52.5501.0 - 15.0Order now ›
Catalogue numberProduct linePillar diameter (µm)Interpillar distance (µm)Column length (cm)Order
COL-trpnano16G1B2 (Duo-Pack)µPAC pillar array52.51Order now ›
Catalogue numberProduct lineFor use withOrder
EMI-flexionBμPAC Flex iON Connect ESI-MS: Interface to connect μPAC nanoLC columns to a Nanospray Flex Series ion sourceμPAC nano columnsOrder now ›
EMI-easysprayBμPAC Compatible EASY-Spray Emitter: Pre-assembled EASY-Spray emitter, grounding union, and transfer line to connect μPAC nanoLC columns to an EASY-Spray sourceμPAC nano columnsOrder now ›

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