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Low-flow chromatography is ideal when detailed sample information is required from small sample volumes, especially for bottom-up and top-down proteomics research. Compared with analytical flow LC-MS (>100 µL/min), the signal with low-flow (<100 µL/min) can be many times higher. Low-flow columns—also known as micro, nano, and capillary columns—have a smaller diameter compared to analytical columns.
Thermo Scientific nano-, capillary-, and micro-flow columns offer excellent sensitivity and resolution in easy-to-use formats:
Learn more about the power of Thermo Scientific low-flow columns and discover column designs that ensure near zero-dead volume connections to give you confidence in your data quality. Thermo Scientific low-flow (U)HPLC columns — technology that’s beyond brilliant.
Low-flow (U)HPLC column formats | ||
EASY-Spray (U)HPLC Columns | Double nanoViper (U)HPLC Columns | µPAC Neo HPLC Columns |
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Choose an EASY-Spray column when: | What makes an EASY-Spray column special? |
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| Unique design provides uncompromised performance in an ease of use format for nano and capillary LC-MS analysis. Features for optimum data quality:
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The Thermo Scientific EASY-Spray PepMap Neo UHPLC columns are perfect for bottom-up proteomics. Packed at higher pressure and rated to 1500 bar, they provide consistent column-to-column performance, long column lifetime, and excellent efficiency. These benefits are true at any pressure.
Catalog number | Product line | Particle size (µm) | Column ID (µm) | Column length (mm) | Order |
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ES75150PN | PepMap Neo columns | 2 | 75 | 150 | Order now › |
ES75500PN | PepMap Neo columns | 2 | 75 | 500 | Order now › |
ES75750PN | PepMap Neo columns | 2 | 75 | 750 | Order now › |
Also see: EASY-Spray PepMap HPLC columns
The Thermo Scientific MAbPac RP capillary column is best suited for the characterization of intact proteins in top-down proteomics, clinical and anti-doping applications where sample amount is limited, or sensitivity is crucial.
Catalog number | Product line | Particle size (µm) | Column length (mm) | Column ID (µm) | Order |
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ES907 | MAbPac RP column | 4 | 150 | 150 | Order now › |
For the best performance from your EASY-Spray column, consider investing in the following accessories.
Catalog number | Product line | Particle size (µm) | Column ID (µm) | Media bed length (mm) | Trap length (mm) | Union type | Order |
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174500 | PepMap Neo Trap Cartridge 3PK | 5 | 300 | 5 | N/A | N/A | Order now › |
164199 | PepMap nanotrap 500 bar 2PK | 5 | 100 | 20 | 150 | Nut/sleeve | Order now › |
164750 | PepMap nanotrap 500 bar 2PK | 5 | 100 | 20 | 150 | Double nanoViper | Order now › |
164535 | PepMap nanotrap 500 bar 2PK | 3 | 75 | 20 | 150 | Double nanoViper | Order now › |
164946 | PepMap nanotrap 1200 bar 2PK | 3 | 75 | 20 | 70 | Double nanoViper | Order now › |
164213 | PepMap nanotrap 500 bar 2PK | 5 | 200 | 20 | 150 | Nut/sleeve | Order now › |
Catalog number | Product line | For use with | Order |
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174501 | PEEK with nanoViper fittings 30 µm X100 mm 2PK 1500 bar | Low-flow PepMap columns | Order now › |
174502 | Trap holder + nanoViper fittings kit 1500 bar | Low-flow PepMap columns | Order now › |
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Choose these columns when: | What makes these columns special? |
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| These stand-alone nano-, capillary, and micro-flow columns are:
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Use these columns for peptide bottom-up proteomics studies. They are packed at higher pressure and rated a 1500 bar and provide consistent column-to-column performance, long column lifetime, and excellent efficiency. These benefits are true at any pressure.
Catalog number | Product line | Particle size (µm) | Column ID (µm) | Column length (mm) | Order |
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DNV75150PN | PepMap Neo column | 2 | 75 | 150 | Order now › |
DNV75500PN | PepMap Neo column | 2 | 75 | 500 | Order now › |
DNV75750PN | PepMap Neo column | 2 | 75 | 750 | Order now › |
Also available: Single nanoViper PepMap C18 HPLC columns
The Thermo Scientific MAbPac RP capillary column is best suited for the characterization of intact proteins in top-down proteomics, clinical, and anti-doping applications where sample amount is limited, or sensitivity is crucial.
Catalog number | Product line | Particle size (µm) | Column ID (µm) | Column length (mm) | Order |
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164947 | MAbPac RP column | 4 | 150 | 150 | Order now › |
Catalog number | Product line | Particle size (µm) | Column ID (µm) | Column length (mm) | Order |
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16726-157569 | Amide HILIC column | 2.6 | 75 | 150 | Order now › |
These emitters, nanoViper tubing kits, and unions offer easy connection from your LC system to an EASY-Spray source.
Catalog number | Product line | For use with | Order |
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6040.2304 | Two Viper unions | Double nanoViper columns | Order now › |
6041.5260 | nanoViper tubing 20 µm x 550 mm | Double nanoViper columns | Order now › |
ES993 | Emitter: 10 µm I.D. | Double nanoViper columns | Order now › |
ES994 | Emitter: 15 µm I.D | Double nanoViper columns | Order now › |
For the best performance from your double nanoViper column consider investing in the following accessories.
Catalog number | Product line | Particle size (µm) | Column ID (µm) | Media bed length (mm) | Trap length (mm) | Union type | Order |
---|---|---|---|---|---|---|---|
174500 | PepMap Neo Trap Cartridge 3PK | 5 | 300 | 5 | N/A | N/A | Order now › |
164199 | PepMap nanotrap 500 bar 2PK | 5 | 100 | 20 | 150 | Nut/sleeve | Order now › |
164750 | PepMap nanotrap 500 bar 2PK | 5 | 100 | 20 | 150 | Double nanoViper | Order now › |
164535 | PepMap nanotrap 500 bar 2PK | 3 | 75 | 20 | 150 | Double nanoViper | Order now › |
164946 | PepMap nanotrap 1200 bar 2PK | 3 | 75 | 20 | 70 | Double nanoViper | Order now › |
164213 | PepMap nanotrap 500 bar 2PK | 5 | 200 | 20 | 150 | Nut/sleeve | Order now › |
Catalog number | Product line | For use with | Order |
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174501 | PEEK with nanoViper fittings 30 µm X100 mm 2PK 1500 bar | Low-flow PepMap Neo columns | Order now › |
174502 | Trap holder + nanoViper fittings kit 1500 bar | Low-flow PepMap Neo columns | Order now › |
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Choose a μPAC Neo HPLC column when: | What makes µPAC Neo HPLC columns special? |
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| The unique separation path provides:
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These HPLC columns are ideally suited for bottom-up proteomics where resolution is critical to the success of the analysis. Our µPAC Neo columns provide excellent column-to-column reproducibility and peak capacity at a wide range of flow rates and sample amounts.
Catalogue number | Product line | Column length (cm) | Flow rate range (nL/min) | Sample loading | Gradient time | Order |
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COL-NANO050NEOB | µPAC Neo | 50 | 100-750 | < 1 μg | 15 – 120 min | Order now › |
COL-NANO110NEOB | µPAC Neo | 110 | 100-750 | < 4 μg | > 90 min | Order now › |
COL-LOLO050NEOB | µPAC Neo low-load | 50 | 100-750 | < 10 ng | 15 – 90 min | Order now › |
Catalogue number | Product line | For use with | Flow rate range (μL/min) | Sample loading | Order |
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COL-TRPLOLONEOB2 (Duo-Pack) | μPAC Neo low-load trapping column | μPAC Neo low-load HPLC column | 100-750 | < 10 ng | Order now › |
Catalogue number | Product line | For use with | Order |
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6040.2304 | Union for Viper and nanoViper Tubing | Double nanoViper and μPAC Neo Columns | Order now › |
6041.5260 | nanoViper tubing 20 μm x 550 mm | Double nanoViper and μPAC Neo Columns | Order now › |
ES993 | Nano EASY-Spray emitter, 10 μm I.D. | Double nanoViper and μPAC Neo Columns | Order now › |
These HPLC columns are ideally suited for bottom-up proteomics where resolution is critical to the success of the analysis. Our µPAC HPLC columns provide excellent column-to-column reproducibility and peak capacity at a wide range of flow rates.
Catalogue number | Product line | Pillar diameter (µm) | Interpillar distance (µm) | Column length (cm) | Flow rate range (µL/min) | Order |
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COL-nano050G1B | µPAC pillar array | 5 | 2.5 | 50 | 0.1 – 2.0 | Order now › |
COL-nano200G1B | µPAC pillar array | 5 | 2.5 | 200 | 0.1 - 1.0 | Order now › |
COL-cap050G1B | µPAC pillar array (capillary flow) | 5 | 2.5 | 50 | 1.0 - 15.0 | Order now › |
Catalogue number | Product line | Pillar diameter (µm) | Interpillar distance (µm) | Column length (cm) | Order |
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COL-trpnano16G1B2 (Duo-Pack) | µPAC pillar array | 5 | 2.5 | 1 | Order now › |
Catalogue number | Product line | For use with | Order |
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EMI-flexionB | μPAC Flex iON Connect ESI-MS: Interface to connect μPAC nanoLC columns to a Nanospray Flex Series ion source | μPAC nano columns | Order now › |
EMI-easysprayB | μPAC Compatible EASY-Spray Emitter: Pre-assembled EASY-Spray emitter, grounding union, and transfer line to connect μPAC nanoLC columns to an EASY-Spray source | μPAC nano columns | Order now › |
The all-in-one nano-, capillary-, and micro-flow LC system for high sensitivity LCMS workflows.
Webinar: Workflows for robust and sensitive separation of mAbs, intact proteins at subunit and peptide level by LC-MS
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