Your solution for difficult separations in both reversed phase and normal phase HPLC and LC/MS applications

Porous graphitic carbon (PGC, Hypercarb™) has unique properties as a stationary phase in high-performance liquid chromatography (HPLC). Its chemical surface properties distinguish it from more conventional LC packings such as bonded-silica gels and polymers. PGC behaves as a strongly retentive alkyl-bonded silica gel for non-polar analytes. However, its retention and selectivity behavior towards polar and structurally-related compounds is very different from conventional C18 columns.

Features of Hypercarb columns

Thermo Scientific™ Hypercarb Porous Graphitic Carbon LC Columns offer:

  • Exceptional retention of very polar analytes
  • Separation for structurally-related substances
  • pH stablility from 0 to 14, and are not affected by aggressive mobile phases
  • Ideal solution for high-temperature applications

 

Physical properties of PGC
Property To meet requirement of
Particle shape: Spherical No micropores
Specific surface area: 120 m2/g Retention linearity and loading capacity
Median pore diameter: 250 Å Mass transfer for wide range of analyte shapes and sizes
Pore volume: 0.7 m3/g  
Mean particle diameters: 3 µm, 5 µm Analytical HPLC columns
7 µm Preparative HPLC columns
30 µm SPE applications
Porosity: 75% Mass transfer within particles
% C: 100% Chemical stability
Mechanical strength: <400=""> Operational particle stability; pressure gradients in packing process
Hypercarb columns literature

For access to the following literature please register now. 

Applications Notebook, Issue 1

  • Online interactive version
  • Apple™ iPad™–optimized version
  • PDF (3.67 MB)

Method Development Guide for Hypercarb Column

  • PDF (1.31 MB)

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