Hypersil GOLD HPLC and UHPLC Columns | Thermo Fisher Scientific

Hypersil GOLD HPLC and UHPLC Columns – Designed for optimum chromatography

Consistency you can rely on, results you can trust

Enjoy the range and capabilities of these state-of-the-art columns and gain confidence in the accuracy and quality of your analytical data. The Thermo Scientific Hypersil GOLD family of liquid chromatography (LC) columns is the culmination of 40 years of experience in the media and column product development and manufacturing.

Peak symmetry

Hypersil GOLD columns produce highly symmetrical peaks, which facilitate the detection of low-level impurities that elute immediately after the main component. When there is even minor tailing, small impurity peaks can elute on the shoulder of the main peak, complicating the integration process.

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Conditions

Column: Hypersil GOLD, 5μm, 150 × 4.6mm
Mobile Phase: A: 0.1% ammonia pH 10.6
B: Methanol + 0.1% ammonia
Gradient: 5–100% B in 15 min
Flow Rate: 1.0mL/min
Injection Volume: 10μL
Detection: UV @ 254nm
Temperature: 30 °C

Hypersil GOLD column phase properties

Phase	Phase Properties
Hypersil GOLD Peptide HPLC Columns	Consistent retention of all peptides, including hydrophilic ones, and resolve deamidated species
Hypersil GOLD C18 Selectivity LC Columns	Excellent peak shape for all analyte types.
Hypersil GOLD C8 LC Columns	Similar selectivity to C18 columns, but with reduced retention.
Hypersil GOLD C4 LC Columns	Similar selectivity to C18 and C8 columns, but with reduced retention.
Hypersil GOLD aQ LC Columns	Polar endcapped C18 columns, stable in 100% aqueous mobile phase, providing enhanced retention and resolution of polar analytes.
Hypersil GOLD PFP LC Columns	Perfluorinated phenyl columns offering alternative selectivity. The fluorine atoms around the phenyl ring enhance pi-pi interactions with aromatic molecules.
Hypersil GOLD Phenyl LC Columns	Excellent retention and unique selectivity for aromatic analytes.
Hypersil GOLD Cyano LC Columns	Alternative selectivity with lower hydrophobicity; can also be used for normal phase separations.
Hypersil GOLD Amino LC Columns	Can be used in reversed phase, normal phase, ion exchange, and HILIC modes; particularly useful for separating carbohydrates.
Hypersil GOLD AX LC Columns	Separation of smaller proteins, peptides, anionic species, and polar molecules.
Hypersil GOLD SAX LC Columns	A quaternary amino ion exchange ligand ideally suited to separating small polar organic analytes in aqueous mobile phases.
Hypersil GOLD Silica LC Columns	Separation of nonpolar and moderately polar organic compounds by normal phase chromatography.
Hypersil GOLD PEI HILIC LC Columns	Enhanced retention of polar and hydrophilic analytes that are problematic using reversed phase columns.

Important product information

Hypersil GOLD HPLC columns technical guide

Hypersil GOLD columns are based on improved, highly pure silica and a novel proprietary derivatization and endcapping procedure using alkyl chain chemistry.

Hypersil GOLD HPLC columns phase overview

The capabilities of this state-of-the-art family of columns, with numerous chemistries and a range of particle sizes and hardware formats, meet the challenges of modern chromatography.

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2022/2023 HPLC/UHPLC Columns Catalog

Access the latest interactive version of our Chromatography Consumables & Columns Catalog. Our enhanced online version features easier access to product information, key application notes and brand-new selector tools.

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