Acclaim HPLC Columns

Your go-to HPLC columns for separating complex mixtures

Thermo Scientific Acclaim HPLC Columns have a smaller pore size and higher surface area to deliver the high resolution you need for high-efficiency separations. Choose from a broad variety of functional groups including:

  • Conventional reversed-phase C18 and polar embedded phases
  • Mixed-mode phases
  • Application-specific phases

Thermo Fisher Scientific designed and manufactured all Acclaim columns to tight specifications to ensure you get consistent performance.


Acclaim HPLC column families

Column Use for Data sheet
Acclaim 120 C18 columns High efficiency reversed-phase columns for small molecule and complex mixtures separations, including LC-MS Acclaim 120 C18 data sheet
Acclaim 120 C8 columns Reversed-phase columns for small molecule with intermediate hydrophobic retention Acclaim 120 C8 data sheet
Acclaim 300 C18 columns Wide pore columns for LC-MS life science applications; protein and peptides Acclaim 300 C18 data sheet
Acclaim Polar Advantage (PA) columns Polar-embedded reversed-phase columns for separating polar and nonpolar analytes with 100% aqueous mobile phase Acclaim PA data sheet
Acclaim Polar Advantage (PA2) columns Polar-embedded columns with enhanced hydrolytic stability pH 1.5 – 10, excellent for polar and non-polar analytes with 100% aqueous mobile phase and LC-MS Acclaim PA2 data sheet
Acclaim C30 columns Very high hydrophobic retention; good for separating structurally related isomers Acclaim C30 data sheet
Acclaim Phenyl-1 columns Polar reversed-phase columns with high aromatic selectivity Acclaim Phenyl-1 data sheet
Acclaim HILIC-10 columns Designed for unique selectivity of hydrophilic molecules Acclaim HILIC-10 data sheet
Specifications for Acclaim reversed-phase & general-purpose HPLC columns
  Acclaim
120 C18
Acclaim
120 C8
Acclaim
300 C18
Acclaim PA Acclaim PA2 Acclaim C30 Acclaim
Phenyl-1
Acclaim
HILIC-10
Bonded Phase Octadecylsilane Octylsilane Octadecylsilane Embedded sulfonamide Embedded Amide C30 alkylsilane Phenyl Proprietary
USP Type L1 L7 L1 L60 L60 L62 L11 na
End-capped Yes Yes Yes Yes Yes Proprietary Yes  
Starting Material Ultrapure silica Ultrapure silica Ultrapure silica Ultrapure silica Ultrapure silica Ultrapure silica Ultrapure silica Ultrapure silica
Particle Shape Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical
Particle Sizes 2.2 μm,
3 μm,
5 μm
2.2 μm,
3 μm,
5 μm
3 μm 3 μm,
5 μm
3 μm,
5 μm
3 μm,
5 μm
3 μm,
5 μm
3 μm,
5 μm
Average Pore Diameter 120 Å 120 Å 300 Å 120 Å 120 Å 200 Å 120 Å 120 Å
Surface Area 300 m2/g 300 m2/g 100 m2/g 300 m2/g 300 m2/g 200 m2/g 300 m2/g 300 m2/g
Total Carbon Content 18% 11% 7% 17% 17%      
Column Use for Data sheet
Acclaim Trinity P1 columns
  • Reversed-phase, anion-exchange, and cation exchange functionalities
  • For superior resolution of API and counter ions, as well as complex mixtures
Acclaim Trinity P1 data sheet
Acclaim Trinity P2 columns
  • HILIC, anion-exchange, and cation exchange functionalities
  • For complex mixtures including separation pharmaceutical counterion; mono- and divalent cations and anions
Acclaim Trinity P2 data sheet
Acclaim Mixed-Mode WAX-1 columns
  • Reversed-phase and anion-exchange combined functionality
  • For separating anionic molecules with powerful adjustable selectivity control
Acclaim Mixed-Mode WAX-1 data sheet
Acclaim Mixed-Mode WCX-1 columns
  • Reversed-phase and cation-exchange combined functionality
  • For separating cationic molecules with powerful adjustable selectivity control
Acclaim Mixed-Mode WCX-1 data sheet
Acclaim Mixed-Mode HILIC-1 columns
  • Combined capability to operate in either reversed-phase or HILIC mode
Acclaim Mixed-Mode HILIC-1 data sheet
Specifications for Acclaim mixed-mode HPLC columns
  Acclaim Trinity P1 Acclaim Trinity P2 Acclaim Mixed-Mode WCX-1 Acclaim Mixed-Mode WAX-1 Acclaim Mixed-Mode HILIC-1
Functionality Reversed-phase + weak anion exchange + strong cation exchange HILIC + strong anion exchange + weak cation exchange Reversed-phase + weak cation exchange reversed-phase + weak anion exchange Reversed-phase + HILIC
USP Type n.a. n.a. L85 L78 n.a.
Starting Material Ultra-pure silica Ultra-pure silica Ultra-pure silica Ultra-pure silica Ultra-pure silica
Particle Shape Spherical Spherical Spherical Spherical Spherical
Particle Sizes 3 μm,
5 μm
3 μm,
5 μm
3 μm 3 μm,
5 μm
3 μm,
5 μm
Average Pore Diameter 120 Å 120 Å 300 Å 120 Å 120 Å
Surface Area  300 m2/g 300 m2/g 100 m2/g 300 m2/g 300 m2/g
Column Use for Data sheet
Acclaim Organic Acid HPLC Columns Fast organic acid analysis; including both aromatic and aliphatic organic acids. Acclaim Organic Acid data sheet
Acclaim Surfactant HPLC Columns Designed for the separation of surfactants including anionic, non-ionic, cationic, and amphoteric surfactants in various matrices. The Surfactant Plus is compatible with LC-MS instrumentation Acclaim Surfactant data sheet
Acclaim Surfactant Plus columns Designed for the separation of surfactants including anionic, non-ionic, cationic, and amphoteric surfactants in various matrices. The Surfactant Plus column is compatible with LC-MS instrumentation Acclaim Surfactant Plus data sheet
Acclaim Explosives E2 columns Specialty columns to comply with EPA Method 8330; baseline resolution of all 14 explosive residues targeted by the EPA  
Acclaim Trinity Q1 columns Excellent for trace analysis of diquat and paraquat  
Acclaim Carbonyl columns Separation of DNPH derivatives of aldehydes and ketones; including regulated methods U.S. EPA 554, EPA 8315, EPA 1667, EPA TO-11, and CARB 1004. Acclaim Carbonyl data sheet
Acclaim Carbamate columns Separation of carbamate pesticides specified in U.S. EPA Method 531.2 Acclaim Carbamate data sheet
Acclaim AmG C18 columns Reversed-phase separation of aminoglycoside antibiotics. Acclaim AmG C18 data sheet
Specifications for Acclaim application-specific columns
  Acclaim Organic Acid Acclaim Surfactant Acclaim Surfactant Plus Acclaim Explosives E2 Acclaim Carbonyl Acclaim Carbamate Acclaim AmG Acclaim Trinity Q1
End-capped Yes Yes Yes Yes Yes Yes Yes Yes
Particle Shape Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical
Particle Size 3 μm,
5 μm
3 μm,
5 μm
3 μm,
5 μm
2.2 μm,
3 μm,
5 μm
2.2 μm,
3 μm,
5 μm
2.2 μm,
3 μm,
5 μm
3 μm 3 μm,
5 μm
Average Pore Diameter 120 Å 120 Å 120 Å 300 Å 120 Å 120 Å 300 Å 300 Å
Surface Area 300 m2/g 300 m2/g 300 m2/g 100 m2/g 300 m2/g 300 m2/g 100 m2/g 100 m2/g
Column Use for Data sheet
Acclaim SEC-1000 and Acclaim SEC-300 Separation of water-soluble polymers using resin based columns. Acclaim SEC columns data sheet
Specifications for Acclaim size exclusion columns (SEC)
  Acclaim SEC-300 Acclaim SEC-1000
Substrate Hydrophilic polymethacrylate resin Hydrophilic polymethacrylate resin
Particle Shape Spherical Spherical
Particle Size 5 μm 7 μm
Pore Size 300 Å (multi-pore) 1000 Å (multi-pore)
Separation range for PEO* 100 – 50,000 Daltons 1,000 – 1,000,000 Daltons
Exclusion limit for PEO* 50,000 – 150,000 Daltons 3,000,000 – 7,500,000 Daltons
Acclaim Column Selection Guide

Related HPLC columns

Accucore HPLC columns

Accucore HPLC columns

Solid core particles in Accucore HPLC columns deliver fast separations with superb resolution.

See Accucore HPLC Columns

Hypersil GOLD HPLC columns

Hypersil GOLD HPLC columns

General-purpose columns for exceptional peak shape and accuracy in LC and MS applications.

See Hypersil Gold HPLC Columns


Accelerating Chromatography CCS
Workflow product selector

Select Chromatography Consumables and Columns for your Pharma, Biopharma, Clinical, Food & Beverage or Environmental workflow.

View now