Acclaim HPLC Columns

Your go-to HPLC columns for separating complex mixtures

Thermo Scientific Acclaim HPLC Columns have a smaller pore size and higher surface area to deliver the high resolution you need for high-efficiency separations. Choose from a broad variety of functional groups including:

  • Conventional reversed-phase C18 and polar embedded phases
  • Mixed-mode phases
  • Application-specific phases

Thermo Fisher Scientific designed and manufactured all Acclaim columns to tight specifications to ensure you get consistent performance.


Acclaim HPLC column families

ColumnUse forData sheet
Acclaim 120 C18 columnsHigh efficiency reversed-phase columns for small molecule and complex mixtures separations, including LC-MSAcclaim 120 C18 data sheet
Acclaim 120 C8 columnsReversed-phase columns for small molecule with intermediate hydrophobic retentionAcclaim 120 C8 data sheet
Acclaim 300 C18 columnsWide pore columns for LC-MS life science applications; protein and peptidesAcclaim 300 C18 data sheet
Acclaim Polar Advantage (PA) columnsPolar-embedded reversed-phase columns for separating polar and nonpolar analytes with 100% aqueous mobile phaseAcclaim PA data sheet
Acclaim Polar Advantage (PA2) columnsPolar-embedded columns with enhanced hydrolytic stability pH 1.5 – 10, excellent for polar and non-polar analytes with 100% aqueous mobile phase and LC-MSAcclaim PA2 data sheet
Acclaim C30 columnsVery high hydrophobic retention; good for separating structurally related isomersAcclaim C30 data sheet
Acclaim HILIC-10 columnsDesigned for unique selectivity of hydrophilic molecules 
Specifications for Acclaim reversed-phase & general-purpose HPLC columns
 Acclaim
120 C18
Acclaim
120 C8
Acclaim
300 C18
Acclaim PAAcclaim PA2Acclaim C30Acclaim
Phenyl-1
Acclaim
HILIC-10
Bonded PhaseOctadecylsilaneOctylsilaneOctadecylsilaneEmbedded sulfonamideEmbedded AmideC30 alkylsilanePhenylProprietary
USP TypeL1L7L1L60L60L62L11na
End-cappedYesYesYesYesYesProprietaryYes 
Starting MaterialUltrapure silicaUltrapure silicaUltrapure silicaUltrapure silicaUltrapure silicaUltrapure silicaUltrapure silicaUltrapure silica
Particle ShapeSphericalSphericalSphericalSphericalSphericalSphericalSphericalSpherical
Particle Sizes2.2 μm,
3 μm,
5 μm
2.2 μm,
3 μm,
5 μm
3 μm3 μm,
5 μm
3 μm,
5 μm
3 μm,
5 μm
3 μm,
5 μm
3 μm,
5 μm
Average Pore Diameter120 Å120 Å300 Å120 Å120 Å200 Å120 Å120 Å
Surface Area300 m2/g300 m2/g100 m2/g300 m2/g300 m2/g200 m2/g300 m2/g300 m2/g
Total Carbon Content18%11%7%17%17%   
ColumnUse forData sheet
Acclaim Trinity P1 columns
  • Reversed-phase, anion-exchange, and cation exchange functionalities
  • For superior resolution of API and counter ions, as well as complex mixtures
Acclaim Trinity P1 data sheet
Acclaim Trinity P2 columns
  • HILIC, anion-exchange, and cation exchange functionalities
  • For complex mixtures including separation pharmaceutical counterion; mono- and divalent cations and anions
Acclaim Trinity P2 data sheet
Acclaim Mixed-Mode WAX-1 columns
  • Reversed-phase and anion-exchange combined functionality
  • For separating anionic molecules with powerful adjustable selectivity control
Acclaim Mixed-Mode WAX-1 data sheet
Acclaim Mixed-Mode WCX-1 columns
  • Reversed-phase and cation-exchange combined functionality
  • For separating cationic molecules with powerful adjustable selectivity control
Acclaim Mixed-Mode WCX-1 data sheet
Acclaim Mixed-Mode HILIC-1 columns
  • Combined capability to operate in either reversed-phase or HILIC mode
Acclaim Mixed-Mode HILIC-1 data sheet
Specifications for Acclaim mixed-mode HPLC columns
 Acclaim Trinity P1Acclaim Trinity P2Acclaim Mixed-Mode WCX-1Acclaim Mixed-Mode WAX-1Acclaim Mixed-Mode HILIC-1
FunctionalityReversed-phase + weak anion exchange + strong cation exchangeHILIC + strong anion exchange + weak cation exchangeReversed-phase + weak cation exchangereversed-phase + weak anion exchangeReversed-phase + HILIC
USP Typen.a.n.a.L85L78n.a.
Starting MaterialUltra-pure silicaUltra-pure silicaUltra-pure silicaUltra-pure silicaUltra-pure silica
Particle ShapeSphericalSphericalSphericalSphericalSpherical
Particle Sizes3 μm,
5 μm
3 μm,
5 μm
3 μm3 μm,
5 μm
3 μm,
5 μm
Average Pore Diameter120 Å120 Å300 Å120 Å120 Å
Surface Area 300 m2/g300 m2/g100 m2/g300 m2/g300 m2/g
ColumnUse forData sheet
Acclaim Organic Acid HPLC ColumnsFast organic acid analysis; including both aromatic and aliphatic organic acids.Acclaim Organic Acid data sheet
Acclaim Surfactant HPLC ColumnsDesigned for the separation of surfactants including anionic, non-ionic, cationic, and amphoteric surfactants in various matrices. The Surfactant Plus is compatible with LC-MS instrumentationAcclaim Surfactant data sheet
Acclaim Surfactant Plus columnsDesigned for the separation of surfactants including anionic, non-ionic, cationic, and amphoteric surfactants in various matrices. The Surfactant Plus column is compatible with LC-MS instrumentationAcclaim Surfactant Plus data sheet
Acclaim Explosives E2 columnsSpecialty columns to comply with EPA Method 8330; baseline resolution of all 14 explosive residues targeted by the EPA 
Acclaim Trinity Q1 columnsExcellent for trace analysis of diquat and paraquat 
Acclaim Carbonyl columnsSeparation of DNPH derivatives of aldehydes and ketones; including regulated methods U.S. EPA 554, EPA 8315, EPA 1667, EPA TO-11, and CARB 1004.Acclaim Carbonyl data sheet
Acclaim Carbamate columnsSeparation of carbamate pesticides specified in U.S. EPA Method 531.2Acclaim Carbamate data sheet
Acclaim AmG C18 columnsReversed-phase separation of aminoglycoside antibiotics.Acclaim AmG C18 data sheet
Specifications for Acclaim application-specific columns
 Acclaim Organic AcidAcclaim SurfactantAcclaim Surfactant PlusAcclaim Explosives E2Acclaim CarbonylAcclaim CarbamateAcclaim AmGAcclaim Trinity Q1
End-cappedYesYesYesYesYesYesYesYes
Particle ShapeSphericalSphericalSphericalSphericalSphericalSphericalSphericalSpherical
Particle Size3 μm,
5 μm
3 μm,
5 μm
3 μm,
5 μm
2.2 μm,
3 μm,
5 μm
2.2 μm,
3 μm,
5 μm
2.2 μm,
3 μm,
5 μm
3 μm3 μm,
5 μm
Average Pore Diameter120 Å120 Å120 Å300 Å120 Å120 Å300 Å300 Å
Surface Area300 m2/g300 m2/g300 m2/g100 m2/g300 m2/g300 m2/g100 m2/g100 m2/g
ColumnUse forData sheet
Acclaim SEC-1000 and Acclaim SEC-300Separation of water-soluble polymers using resin based columns.Acclaim SEC columns data sheet
Specifications for Acclaim size exclusion columns (SEC)
 Acclaim SEC-300Acclaim SEC-1000
SubstrateHydrophilic polymethacrylate resinHydrophilic polymethacrylate resin
Particle ShapeSphericalSpherical
Particle Size5 μm7 μm
Pore Size300 Å (multi-pore)1000 Å (multi-pore)
Separation range for PEO*100 – 50,000 Daltons1,000 – 1,000,000 Daltons
Exclusion limit for PEO*50,000 – 150,000 Daltons3,000,000 – 7,500,000 Daltons

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