Clogging has met its match

  • Minimize the hassle, frustration, and sample-loss associated with a clogged instrument
  • Investigate difficult samples and large, clumpy cells, including cancer cells, cardiomyocytes, and adipocytes, which were previously not amenable to flow cytometry

Request a demo   Request a quote

3D Demo


Efficient. Flexible. Transformative.

The 2018 Attune NxT Flow Cytometer system makes multiparametric flow cytometry available to both new and experienced researchers. Experience the difference acoustic assisted hydrodynamic focusing technology brings to your workflow. Designed for faster experimental run times, this compact flow cytometer offers proven, rapid and accurate analysis for a broad range of sample types.

2018-attune-355

A flow cytometer designed with your research in mind

  • Conserve sample and quickly run experiments with sample flow rates ranging from 12.5–1,000 μL/min
  • Intuitive software interface and easy-to-follow workflow that is quick to learn, teach and use
  • Save time and minimize cell handling with faster protocols
  • Expand your capabilities with up to 6 channels of detection off the violet laser
Whole blood protocol workflow

Freedom of choice


An evolutionary flow cytometer


Automation for any lab

Improve your workflow for fully automated sample acquisition from 96-well and 384-well microtiter plates. There are no physical changes to the system needed in order to run plate based experiments—everything is controlled in the software.

2018 Attune NxT instrument options

This grow-with-you system can be customized with up to 4 lasers and 14 colors and now up to 6 channels off of the violet laser. The optical system was designed to provide optimal signal and has the range to detect very bright as well as dim signals. The Attune NxT Flow Cytometer allows researchers the freedom to build multicolor panels, accommodating common fluorophores and dyes. Build your multicolor panel for the Attune NxT flow cytometer with our superior reagents and antibodies.

Attune NxT Flow Cytometer configuration options

Lasers Laser configuration
(Cat. No.)
Lasers included, No. of detection channels Total detection channels*
Violet, 405 nm Blue, 488 nm Yellow, 561 nm Green,
532 nm
Red, 637 nm
1 Blue
(A24864)
Available as upgrade†
4
Available as upgrade† Available as upgrade† Available as upgrade† 6
2 Blue/green
(A28995)
Available as upgrade†
3
 
4
Available as upgrade† 9
Blue/yellow
(A24861)
Available as upgrade†
3
4
  Available as upgrade† 9
Blue/red
(A24863)
Available as upgrade†
4
Available as upgrade† Available as upgrade†
3
9
Blue/violet
(A24862)
4
4
Available as upgrade† Available as upgrade† Available as upgrade† 10
Blue/violet 6
(A29002)
6
3
Available as upgrade†   Available as upgrade† 11
3 Blue/green/red
(A28997)
Available as upgrade†
3
 
4
3
12
Blue/red/yellow (A28993) Available as upgrade† 3 4   3 12
Blue/green/violet
(A28999)
4
3
 
4
Available as upgrade† 13
Blue/violet/yellow (A24859)
4
3
4
 
Available as upgrade† 13
Blue/red/violet
(A24860)
4
4
Available as upgrade† Available as upgrade†
3
13
Blue/red/violet 6
(A29003)
6
3
Available as upgrade†  
3
14
4 Blue/red/violet/green
(A29001)
4
3
 
4
3
16
Blue/red/yellow/violet
(A24858)
4
3
4
 
3
16
Blue/red/yellow/violet 6
(A29004)
6
2
3
 
3
16
* Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel.
† Attune NxT Flow Cytometer owners wishing to purchase a laser upgrade should request a quote here: Yellow Laser Upgrade, Violet Laser UpgradeRed Laser Upgrade, or Green Laser Upgrade. After we receive your quote request, a technical sales specialist will contact you about upgrade options.
Optics
  • Laser power (as shown in table below)
    Laser Wavelength (nm) Beam-shaping optics (BSO)* (mW) Diode power** (mW)
    Violet 405 50 100
    Blue 488 50 100
    Green 532 100 140
    Yellow 561 50 100
    Red 637 100 140
    * Amount of measured usable laser power after light has gone through the beam optics and shaping filters.
    ** Vendor-specified theoretical maximum.
  • Laser excitation: Optimized excitation for minimized stray laser-line noise and losses to reflection 
  • Laser profile: 10 μm x 50 μm flat-top laser providing robust alignment 
  • Emission filters: Up to 14 color channels with wavelength-tuned photomultiplier tubes (PMTs); user-changeable, keyed filters 
  • Laser separation: 150 μm 
  • Optical alignment: Fixed alignment with prealigned welded fiber; no user maintenance require
  • Onboard thermal-electric cooler: No warm-up delay; fiber isn't affected by on/off 
  • Simmer mode: Instant on/off reduces usage and/or aging by 10x; only keep it "on" when acquiring samples; reports hours of usage
  • Flat top specified at the flow cell: CV <3% over width of flat top
  • Upgradable: Convenient field changes
Fluidics
  • Flow cell: Quartz cuvette gel coupled to 1.2 numerical aperture (NA) collection lens, 200 x 200 μm
  • Sample analysis volume: 20 μL to 4 mL
  • Custom sample flow rates: 12.5–1,000 μL/min
  • Sample delivery: Positive-displacement syringe pump for volumetric analysis
  • Sample tubes: Accommodates tubes from 17 x 100 mm to 8.5 x 45 mm
  • Fluid-level sensing: Active
  • Standard fluid reservoirs: 1.8 L focusing fluid tank, 1.8 L waste tank, 175 mL shutdown solution tank, and 175 mL wash solution tank
  • Fluid storage: All fluids stored within instrument
  • Extended fluidics option: Configuration for 10 L fluid
  • Nominal fluid consumption: 1.8 L/day
  • Automated maintenance cycles: ≤15 min startup and shutdown—deep clean, sanitize, and debubble modes
Performance
  • Fluorescence sensitivity: ≤80 MESF for FITC, ≤30 MESF for PE, ≤70 MESF for APC
  • Fluorescence resolution: CV <3% for the singlet peak of propidium iodide–stained chicken erythrocyte nuclei (CEN)
  • Data acquisition rate: Up to 35,000 events/sec, 34 parameters, based on a 10% coincidence rate per Poisson statistics
  • Maximum electronic speed: 65,000 events/sec with all parameters
  • Carryover: Single-tube format: <1%
  • Forward and side scatter sensitivity: Able to discriminate platelets from noise
  • Forward and side scatter resolution: Optimized to resolve lymphocytes, monocytes, and granulocytes in lysed whole blood
  • Forward scatter: Photodiode detector with 488/10 nm bandpass filter
  • Side scatter: PMT with default 488/10 nm bandpass filter; optional 405/10 nm bandpass filter
  • Fluorescent detectors: 14 individual detectors
  • Electronic pulse: Measured area, height and width pulse for all detectors
  • Violet side scatter resolution: Can be configured for violet side scatter to better resolve particles from noise
  • Minimum particle size: 0.2 μm on side scatter using Bangs Laboratories, Inc.™ Submicron Bead Calibration Kit (Cat. No. 832)
Software
  • Compensation: Full matrix—automated and manual modes, on-plot compensation tools for fine adjustment; use of tubes and wells
  • Flow rate: Precise flow rate control via software; no hardware adjustments
  • Live streaming: Live update of statistics during acquisition of events up to 35,000 events/sec
  • Overlays: Comparative analysis between samples; 3D view
  • Sample recovery: System able to return unused samples
  • Concentration: Direct concentration measurement without use of counting beads
  • Software layout: Fully customizable for each user account
  • Bubble detection technology: Stops automated run to preserve sample integrity
  • Maximum single-event file: 20 million with option to append
  • Heat map: Set up for definition of plate layout; screening view for analysis for tubes and plates
  • Threshold: Up to 4 individual thresholds with user option to apply Boolean logic
  • Gating: Hierarchal gating with the ability to derive gates
  • Smartgate labeling: Option to annotate quad gate names based on fluorophore and target names
  • Voltage: User adjustable
  • Window extensions: User adjustable
  • Area scaling factor (ASF): User adjustable
  • Acquisition settings: Documented in FCS files and maintained upon import
  • Templates: Create from existing experiments—instrument settings, workspaces, run protocols, heat map settings, and compensation settings optimized and defined previously
  • Tube-to-plate conversion: One-click transition from tubes to plates and vice versa; no disassembly, no additional QC, no reboot required for conversion between plates and tubes
  • Graphics resolution: Publication-quality images; support for TIF, PNG, BMP, JPG, GIF, and EMF; quickly copy and paste plots to any external application (e.g., Microsoft™ PowerPoint™ software)
  • User account administration: Administrative creation of individual user accounts with designated roles, advanced setting permissions, management of individual accounts, user time tracking, and sample count
Quality and regulatory
  • Instrument tracking: Automated daily baseline and performance test with Levey-Jennings plots
  • Warranty: 1 year
  • Production verification testing: Each instrument is tested and verified for assembly integrity and performance to specifications
  • Quality management system: Manufacturing standards comply with the requirements of ISO 13485:2003
  • Robust install specifications: Units installed by engineer; preplanning checklist, delivery, installation, and performance validation comply with standardized procedure
  • For Research Use Only
Data management
  • Software requirements: Invitrogen Attune NxT Software
  • Monitor: 23-inch flat panel (1,920 x 1,080 resolution); dual-monitor capability
  • Computer: Minitower desktop
  • Operating system: Windows™ 7 64-bit
  • FCS format: FCS 3.1, 3.0
  • Processor: Intel Core™ i7 processor
  • RAM: 32 GB
  • Hard drives: 2 x 2TB SATA 6.0Gb/s, 7200 RPM, 64MB data burst cache; Controller RAID1, integrated
Installation requirements
  • Electrical requirements: 100–240 VAC, 50/60 Hz, <150 W. Thermo Fisher Scientific certifies that the Attune NxT Flow Cytometer conforms to relevant directives to bear the CE mark. The instrument also conforms to the UL and CAN/CSA general requirements (61010.1). The Attune NxT Flow Cytometer is a Class I laser product per CDRH regulations and EN/IEC 60825.
  • Heat dissipation: <150 W
  • Temperature operating ranges: 15–30°C (59–86°F)
  • Operating humidity: 10–90%, noncondensing
  • Audible noise: <65 dBA at 1.0 m
  • Instrument size (H x W x D): ~40 x 58 x 43 cm (16 x 23 x 17 in.), including fluid bottles
  • Weight: ~29 kg (64 lb)
  • Available configurations (see Attune NxT Flow Cytometer configuration options table)

Simple yet powerful software, easy auto compensation

Invitrogen Attune NxT Software is designed to provide powerful data acquisition and analysis using an intuitive, user-friendly interface. The instrument software features and benefits include:

  • Compensation—fully automated and manual compensation
  • Instrument tracking—automated baseline and performance test for all channels and linearity
  • Automated maintenance—startup (<15 min), shutdown, rinse, wash, debubble, deep clean,
    monthly decontamination
  • User account maintenance—administrative and individual accounts with user log
  • Gates—standard and customizable gates
  • Plot previews—easy workspace setup
  • Zoom filmstrip viewer—easy visualization and navigation of plots to set gates and plot attributes
  • File formats—FCS 3.0 and FCS 3.1
  • Graphics resolution—publication-quality images (customizable)

Learn more about software for the Attune NxT Flow Cytometer

Accessories for the Attune NxT Flow Cytometer

Find laser upgrade and conversion kits to expand the capabilities of your instrument, as well as filter kits and accessory parts.

Attune NxT upgrades Filter Common fluorochromes Cat. No.
Attune NxT Yellow Laser Upgrade Kit 585/16 PE, Alexa Fluor 532, Alexa Fluor 568 100022779
620/15 Alexa Fluor 594, PE-Texas Red, PE-Alexa Fluor 610
695/40 PE-Alexa Fluor 700, TRI-COLOR (PE-Cy5), PE-Cy5.5, Qdot 705
780/60 Qdot 800, PE-Cy 7, PE-Alexa Fluor 750
Attune NxT Red Laser Upgrade Kit 670/14 APC, Alexa Fluor 647, Qdot 655 100022778
720/30 Alexa Fluor 680, Alexa Fluor 700, APC-Alexa Fluor 700, Qdot 705
780/60 APC-Alexa Fluor 750, APC-Cy7, APC-eFluor 780, Qdot 800
Attune NxT Green Laser Upgrade Kit 575/36 PE, Alexa Fluor 532, Alexa Fluor 555, DsRed A32701
620/15 Alexa Fluor 568, Alexa Fluor 594, Qdot 605, PE-Texas Red, PE-Alexa Fluor 610
695/40 Qdot 705, PE-Alexa Fluor 700, TRI-COLOR, PE-Cy5.5
780/60 Qdot 800, PE-Cy7
Attune NxT Violet Laser Upgrade Kit 440/50 Super Bright 436, Alexa Fluor  405, eFluor 450, Pacific Blue 100022777
512/25 eFluor 506, Pacific Green
603/48 Super Bight 600, Pacific Orange, Qdot 605
710/50 Super Bright 702, Qdot 705
Attune NxT Violet 6 Conversion Kit, Blue Laser 530/30 Alexa Fluor 488, FITC A35428
574/26 PE, PE-Alexa Fluor 610, Alexa Fluor 546
695/40 PE-Alexa Fluor 700, PE-Cy5.5, PerCP, PerCP-Cy5.5, Qdot 705, TRI-COLOR
Attune NxT Violet 6 Conversion Kit, Violet Laser 450/40 Super Bright 436, eFluor 450, Pacific  Blue A36569
525/50 eFluor 506, Pacific Green, eFluor 506
610/20 Super Bright 600, Qdot 605, Pacific Orange
660/20 Super Bright 645
710/50 Super Bright 702, Qdot 705
780/60 Super Bright 780
Attune NxT Violet 6 Conversion Kit, Red Laser 670/14 APC, Alexa Fluor 647, Qdot 655 A36571
720/30 Alexa Fluor 680, Alexa Fluor 700, APC-Alexa Fluor 700, Qdot 705
780/60 APC-Alexa Fluor 750, APC-eFluor 780, APC-Cy7, Qdot 800
Attune NxT Violet 6 Conversion Kit, Yellow Laser 585/16 PE, Alexa Fluor 532, Alexa Fluor 568  A36572
620/15 Alexa Fluor 594, PE-Texas Red, PE-Alexa Fluor 610
695/40 PE-Alexa Fluor 700, TRI-COLOR, PE-Cy5.5, Qdot 705
780/60 Qdot 800, PE-Cy 7, PE-Alexa Fluor 750

The Invitrogen Attune NxT Flow Cytometer was developed with acoustic-assisted hydrodynamic focusing technology, and advanced fluidics designed to minimize clogging and effectively handle a broad range of cell types. This allows for a higher degree of data, detail, and throughput that enables processing of a large range of sample types, including large clumpy cells, samples with a low concentration of cells, and precious samples, more quickly and accurately than ever before with no loss in data quality.

The following applications were performed using the Attune NxT Flow Cytometer. 


Literature


Manuals



Cell types successfully analyzed on the Attune NxT Flow Cytometer

Sample type Demonstrated example Published References
Algae Nannochloropsis oculata  
Bacteria Escherichia coli
Bacillus subtilis
Legionella strains
Staphyloccus aureus  
Diatom Phaeodactylum tricornutum
Human Human adenocarcinoma
Human bone marrow (PBMCs)
Human bone osteosarcoma cells (U2OS)
Human cardiomyocytes  
Human corneal epithelial cells  
Human embryonic kidney (293FT)
Human endothelial cells
Human leukocytes  
Human mesenchymal stem cell (hMSC)
Human red blood cells (RBC)  
Human T cell leukemia (Jurkat)
Human whole blood
Mouse Mouse splenocytes
Mouse T and B regulatory cells
Mouse cardiomyocytes
Mouse bone marrow
Mouse lung tissue
Mouse tumor cells
Mouse skeletal muscle
Parasites Plasmodium falciparum
L. amazonensis, T. brucei and T. cruzi
Trypanosoma cruzi
Picophytoplankton Prochlorococcus spp;
Synechococcus spp
 
Plants Arabidopsis thaliana  
Phalaris arundinacea
Solidago canadensis
Yeast Candida albicans
Saccharomyces cerevisiae
Zebra fish Zebra fish organs

On-site service plans at a glance

Increase your lab productivity and uptime, enabling you to focus on what matters most, your research success.

Product AB Complete AB Assurance AB Maintenance
Standard on-site response time Next business day* 2 business days* 3 business days**
Scheduled on-site PM      
Remote instrument diagnostics      
Priority access to technical support
(Monday–Friday, standard business hours)
     
Parts, labor, and travel for repair      
Priority access to remote service engineer      
Post-PM requalification and critical repairs      
Field applications scientist (FAS) consultation      

* Availability limited in some geographic areas.
** After receipt of a purchase order.

Request a quote


Planned maintenance

Planned Maintenance service for the Attune NxT Flow Cytometer includes:

Checks completed during planned maintenance visit:
System maintenance
  • visually inspect all cables
  • clean the interior and the exterior of unit
  • clean the cove tray and visor
  • clean the tube lifter
  • check the tube lifter for proper operation
Computer maintenance
  • run the disk cleanup
  • defragment the disk, only if the operating system indicates that it is recommended
Fluidics system maintenance
  • replace the SIP tube
  • inspect the instrument internally for any traces of salt residue
  • check for internal leaks and damage at all fittings, valves, pumps and syringe pump
  • replace the 1 mL syringe
  • run the system decontamination script, if not previously done by the customer
  • replace both 0.2 micron filters, if not previously done by the customer
Optics maintenance
  • inspect all optical filters. Clean only if necessary
  • verify the laser alignment, including the pinholes and blocker bar
System diagnostics
  • run the System Test and ensure that everything passes
  • ensure that the latest Attune NxT Software version is installed
  • check leak detector functionality
  • record laser hours
Attune NxT Autosampler maintenance (if applicable to unit)
  • run the Attune NxT Autosampler calibration script
  • replace syringe
Instrument performance
  • ensure that the baseline and performance tests pass the specifications

Compliance services

Get timely, cost-effective, and audit-ready documentation managed by a compliance specialist to help ensure your Attune NxT Flow Cytometer system is installed, operating, and performing to the manufacturer’s specifications.

Verify instrument performance and minimize downtime with IQ/OQ/IPV Services

Our factory-trained and experienced engineers will conduct Installation Qualification (IQ), Operational Qualification (OQ), and Instrument Performance Verification (IPV) on the Invitrogen Attune NxT Flow Cytometer system in your lab. Documentation for audits is provided.

Find compliance documents for Installation and Operational Qualification (IQ/OQ), and Instrument Verification (IPV) for your systems


Technical & Application Support

Take the hassle out of your hands with custom solutions

Assay development & labeling services—our custom solutions to help develop the most appropriate assay for your research or discovery target of interest.

Custom antibodies—our custom antibody development, purification, and screening services deliver the quality, consistency, and performance you need.


Education services

Application and instrument training programs are available at our training centers located throughout the world, within your lab, or through web-based instruction. We offer a wide range of courses to build the skills of cellular analysis professionals and users of the Attune NxT Flow Cytometer:

  • Training Center Courses—get away from the day-to-day responsibilities of the lab and dedicate time to focus on your education needs. Courses are hosted at Thermo Fisher Scientific Training Center sites to provide professional, interactive education that includes classroom instruction and hands-on training in the laboratory.
  • Customer Site/content—courses are offered at your location by a Field Application Scientist (FAS) to provide professional, interactive education that includes hands-on instruction in your laboratory.
  • LabCoat Live—learn a specific application in as little as 1 week. Courses provide interactive education via online lectures and a self-paced hands-on experiment using provided protocols and reagents that we ship to your lab.
  • eLearning—discover new areas of interest and learn about life science topics at your own pace; courses provide education via on-demand, pre-recorded webinars.

Learn more about our Flow Cytometry Webinars
Take our T Cell Stimulation and Proliferation eLearning Course


Instrument management

Manage your instrument use and care online with Instrument Management. Whether you are responsible for one instrument or hundreds of instruments, Instrument Management enables you to easily manage your instrument use and care online.

  • 24/7 access to complete instrument service history and field service reports
  • Request online service and service contract quotes
  • Instrument software, patches, and updates
  • Online instrument scheduler for instrument availability
Online instrument scheduler for instrument availability

Find out more about Instrument Management at thermofisher.com/easiertomanage

A flow cytometer designed with your research in mind

  • Conserve sample and quickly run experiments with sample flow rates ranging from 12.5–1,000 μL/min
  • Intuitive software interface and easy-to-follow workflow that is quick to learn, teach and use
  • Save time and minimize cell handling with faster protocols
  • Expand your capabilities with up to 6 channels of detection off the violet laser
Whole blood protocol workflow

Freedom of choice


An evolutionary flow cytometer


Automation for any lab

Improve your workflow for fully automated sample acquisition from 96-well and 384-well microtiter plates. There are no physical changes to the system needed in order to run plate based experiments—everything is controlled in the software.

2018 Attune NxT instrument options

This grow-with-you system can be customized with up to 4 lasers and 14 colors and now up to 6 channels off of the violet laser. The optical system was designed to provide optimal signal and has the range to detect very bright as well as dim signals. The Attune NxT Flow Cytometer allows researchers the freedom to build multicolor panels, accommodating common fluorophores and dyes. Build your multicolor panel for the Attune NxT flow cytometer with our superior reagents and antibodies.

Attune NxT Flow Cytometer configuration options

Lasers Laser configuration
(Cat. No.)
Lasers included, No. of detection channels Total detection channels*
Violet, 405 nm Blue, 488 nm Yellow, 561 nm Green,
532 nm
Red, 637 nm
1 Blue
(A24864)
Available as upgrade†
4
Available as upgrade† Available as upgrade† Available as upgrade† 6
2 Blue/green
(A28995)
Available as upgrade†
3
 
4
Available as upgrade† 9
Blue/yellow
(A24861)
Available as upgrade†
3
4
  Available as upgrade† 9
Blue/red
(A24863)
Available as upgrade†
4
Available as upgrade† Available as upgrade†
3
9
Blue/violet
(A24862)
4
4
Available as upgrade† Available as upgrade† Available as upgrade† 10
Blue/violet 6
(A29002)
6
3
Available as upgrade†   Available as upgrade† 11
3 Blue/green/red
(A28997)
Available as upgrade†
3
 
4
3
12
Blue/red/yellow (A28993) Available as upgrade† 3 4   3 12
Blue/green/violet
(A28999)
4
3
 
4
Available as upgrade† 13
Blue/violet/yellow (A24859)
4
3
4
 
Available as upgrade† 13
Blue/red/violet
(A24860)
4
4
Available as upgrade† Available as upgrade†
3
13
Blue/red/violet 6
(A29003)
6
3
Available as upgrade†  
3
14
4 Blue/red/violet/green
(A29001)
4
3
 
4
3
16
Blue/red/yellow/violet
(A24858)
4
3
4
 
3
16
Blue/red/yellow/violet 6
(A29004)
6
2
3
 
3
16
* Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel.
† Attune NxT Flow Cytometer owners wishing to purchase a laser upgrade should request a quote here: Yellow Laser Upgrade, Violet Laser UpgradeRed Laser Upgrade, or Green Laser Upgrade. After we receive your quote request, a technical sales specialist will contact you about upgrade options.
Optics
  • Laser power (as shown in table below)
    Laser Wavelength (nm) Beam-shaping optics (BSO)* (mW) Diode power** (mW)
    Violet 405 50 100
    Blue 488 50 100
    Green 532 100 140
    Yellow 561 50 100
    Red 637 100 140
    * Amount of measured usable laser power after light has gone through the beam optics and shaping filters.
    ** Vendor-specified theoretical maximum.
  • Laser excitation: Optimized excitation for minimized stray laser-line noise and losses to reflection 
  • Laser profile: 10 μm x 50 μm flat-top laser providing robust alignment 
  • Emission filters: Up to 14 color channels with wavelength-tuned photomultiplier tubes (PMTs); user-changeable, keyed filters 
  • Laser separation: 150 μm 
  • Optical alignment: Fixed alignment with prealigned welded fiber; no user maintenance require
  • Onboard thermal-electric cooler: No warm-up delay; fiber isn't affected by on/off 
  • Simmer mode: Instant on/off reduces usage and/or aging by 10x; only keep it "on" when acquiring samples; reports hours of usage
  • Flat top specified at the flow cell: CV <3% over width of flat top
  • Upgradable: Convenient field changes
Fluidics
  • Flow cell: Quartz cuvette gel coupled to 1.2 numerical aperture (NA) collection lens, 200 x 200 μm
  • Sample analysis volume: 20 μL to 4 mL
  • Custom sample flow rates: 12.5–1,000 μL/min
  • Sample delivery: Positive-displacement syringe pump for volumetric analysis
  • Sample tubes: Accommodates tubes from 17 x 100 mm to 8.5 x 45 mm
  • Fluid-level sensing: Active
  • Standard fluid reservoirs: 1.8 L focusing fluid tank, 1.8 L waste tank, 175 mL shutdown solution tank, and 175 mL wash solution tank
  • Fluid storage: All fluids stored within instrument
  • Extended fluidics option: Configuration for 10 L fluid
  • Nominal fluid consumption: 1.8 L/day
  • Automated maintenance cycles: ≤15 min startup and shutdown—deep clean, sanitize, and debubble modes
Performance
  • Fluorescence sensitivity: ≤80 MESF for FITC, ≤30 MESF for PE, ≤70 MESF for APC
  • Fluorescence resolution: CV <3% for the singlet peak of propidium iodide–stained chicken erythrocyte nuclei (CEN)
  • Data acquisition rate: Up to 35,000 events/sec, 34 parameters, based on a 10% coincidence rate per Poisson statistics
  • Maximum electronic speed: 65,000 events/sec with all parameters
  • Carryover: Single-tube format: <1%
  • Forward and side scatter sensitivity: Able to discriminate platelets from noise
  • Forward and side scatter resolution: Optimized to resolve lymphocytes, monocytes, and granulocytes in lysed whole blood
  • Forward scatter: Photodiode detector with 488/10 nm bandpass filter
  • Side scatter: PMT with default 488/10 nm bandpass filter; optional 405/10 nm bandpass filter
  • Fluorescent detectors: 14 individual detectors
  • Electronic pulse: Measured area, height and width pulse for all detectors
  • Violet side scatter resolution: Can be configured for violet side scatter to better resolve particles from noise
  • Minimum particle size: 0.2 μm on side scatter using Bangs Laboratories, Inc.™ Submicron Bead Calibration Kit (Cat. No. 832)
Software
  • Compensation: Full matrix—automated and manual modes, on-plot compensation tools for fine adjustment; use of tubes and wells
  • Flow rate: Precise flow rate control via software; no hardware adjustments
  • Live streaming: Live update of statistics during acquisition of events up to 35,000 events/sec
  • Overlays: Comparative analysis between samples; 3D view
  • Sample recovery: System able to return unused samples
  • Concentration: Direct concentration measurement without use of counting beads
  • Software layout: Fully customizable for each user account
  • Bubble detection technology: Stops automated run to preserve sample integrity
  • Maximum single-event file: 20 million with option to append
  • Heat map: Set up for definition of plate layout; screening view for analysis for tubes and plates
  • Threshold: Up to 4 individual thresholds with user option to apply Boolean logic
  • Gating: Hierarchal gating with the ability to derive gates
  • Smartgate labeling: Option to annotate quad gate names based on fluorophore and target names
  • Voltage: User adjustable
  • Window extensions: User adjustable
  • Area scaling factor (ASF): User adjustable
  • Acquisition settings: Documented in FCS files and maintained upon import
  • Templates: Create from existing experiments—instrument settings, workspaces, run protocols, heat map settings, and compensation settings optimized and defined previously
  • Tube-to-plate conversion: One-click transition from tubes to plates and vice versa; no disassembly, no additional QC, no reboot required for conversion between plates and tubes
  • Graphics resolution: Publication-quality images; support for TIF, PNG, BMP, JPG, GIF, and EMF; quickly copy and paste plots to any external application (e.g., Microsoft™ PowerPoint™ software)
  • User account administration: Administrative creation of individual user accounts with designated roles, advanced setting permissions, management of individual accounts, user time tracking, and sample count
Quality and regulatory
  • Instrument tracking: Automated daily baseline and performance test with Levey-Jennings plots
  • Warranty: 1 year
  • Production verification testing: Each instrument is tested and verified for assembly integrity and performance to specifications
  • Quality management system: Manufacturing standards comply with the requirements of ISO 13485:2003
  • Robust install specifications: Units installed by engineer; preplanning checklist, delivery, installation, and performance validation comply with standardized procedure
  • For Research Use Only
Data management
  • Software requirements: Invitrogen Attune NxT Software
  • Monitor: 23-inch flat panel (1,920 x 1,080 resolution); dual-monitor capability
  • Computer: Minitower desktop
  • Operating system: Windows™ 7 64-bit
  • FCS format: FCS 3.1, 3.0
  • Processor: Intel Core™ i7 processor
  • RAM: 32 GB
  • Hard drives: 2 x 2TB SATA 6.0Gb/s, 7200 RPM, 64MB data burst cache; Controller RAID1, integrated
Installation requirements
  • Electrical requirements: 100–240 VAC, 50/60 Hz, <150 W. Thermo Fisher Scientific certifies that the Attune NxT Flow Cytometer conforms to relevant directives to bear the CE mark. The instrument also conforms to the UL and CAN/CSA general requirements (61010.1). The Attune NxT Flow Cytometer is a Class I laser product per CDRH regulations and EN/IEC 60825.
  • Heat dissipation: <150 W
  • Temperature operating ranges: 15–30°C (59–86°F)
  • Operating humidity: 10–90%, noncondensing
  • Audible noise: <65 dBA at 1.0 m
  • Instrument size (H x W x D): ~40 x 58 x 43 cm (16 x 23 x 17 in.), including fluid bottles
  • Weight: ~29 kg (64 lb)
  • Available configurations (see Attune NxT Flow Cytometer configuration options table)

Simple yet powerful software, easy auto compensation

Invitrogen Attune NxT Software is designed to provide powerful data acquisition and analysis using an intuitive, user-friendly interface. The instrument software features and benefits include:

  • Compensation—fully automated and manual compensation
  • Instrument tracking—automated baseline and performance test for all channels and linearity
  • Automated maintenance—startup (<15 min), shutdown, rinse, wash, debubble, deep clean,
    monthly decontamination
  • User account maintenance—administrative and individual accounts with user log
  • Gates—standard and customizable gates
  • Plot previews—easy workspace setup
  • Zoom filmstrip viewer—easy visualization and navigation of plots to set gates and plot attributes
  • File formats—FCS 3.0 and FCS 3.1
  • Graphics resolution—publication-quality images (customizable)

Learn more about software for the Attune NxT Flow Cytometer

Accessories for the Attune NxT Flow Cytometer

Find laser upgrade and conversion kits to expand the capabilities of your instrument, as well as filter kits and accessory parts.

Attune NxT upgrades Filter Common fluorochromes Cat. No.
Attune NxT Yellow Laser Upgrade Kit 585/16 PE, Alexa Fluor 532, Alexa Fluor 568 100022779
620/15 Alexa Fluor 594, PE-Texas Red, PE-Alexa Fluor 610
695/40 PE-Alexa Fluor 700, TRI-COLOR (PE-Cy5), PE-Cy5.5, Qdot 705
780/60 Qdot 800, PE-Cy 7, PE-Alexa Fluor 750
Attune NxT Red Laser Upgrade Kit 670/14 APC, Alexa Fluor 647, Qdot 655 100022778
720/30 Alexa Fluor 680, Alexa Fluor 700, APC-Alexa Fluor 700, Qdot 705
780/60 APC-Alexa Fluor 750, APC-Cy7, APC-eFluor 780, Qdot 800
Attune NxT Green Laser Upgrade Kit 575/36 PE, Alexa Fluor 532, Alexa Fluor 555, DsRed A32701
620/15 Alexa Fluor 568, Alexa Fluor 594, Qdot 605, PE-Texas Red, PE-Alexa Fluor 610
695/40 Qdot 705, PE-Alexa Fluor 700, TRI-COLOR, PE-Cy5.5
780/60 Qdot 800, PE-Cy7
Attune NxT Violet Laser Upgrade Kit 440/50 Super Bright 436, Alexa Fluor  405, eFluor 450, Pacific Blue 100022777
512/25 eFluor 506, Pacific Green
603/48 Super Bight 600, Pacific Orange, Qdot 605
710/50 Super Bright 702, Qdot 705
Attune NxT Violet 6 Conversion Kit, Blue Laser 530/30 Alexa Fluor 488, FITC A35428
574/26 PE, PE-Alexa Fluor 610, Alexa Fluor 546
695/40 PE-Alexa Fluor 700, PE-Cy5.5, PerCP, PerCP-Cy5.5, Qdot 705, TRI-COLOR
Attune NxT Violet 6 Conversion Kit, Violet Laser 450/40 Super Bright 436, eFluor 450, Pacific  Blue A36569
525/50 eFluor 506, Pacific Green, eFluor 506
610/20 Super Bright 600, Qdot 605, Pacific Orange
660/20 Super Bright 645
710/50 Super Bright 702, Qdot 705
780/60 Super Bright 780
Attune NxT Violet 6 Conversion Kit, Red Laser 670/14 APC, Alexa Fluor 647, Qdot 655 A36571
720/30 Alexa Fluor 680, Alexa Fluor 700, APC-Alexa Fluor 700, Qdot 705
780/60 APC-Alexa Fluor 750, APC-eFluor 780, APC-Cy7, Qdot 800
Attune NxT Violet 6 Conversion Kit, Yellow Laser 585/16 PE, Alexa Fluor 532, Alexa Fluor 568  A36572
620/15 Alexa Fluor 594, PE-Texas Red, PE-Alexa Fluor 610
695/40 PE-Alexa Fluor 700, TRI-COLOR, PE-Cy5.5, Qdot 705
780/60 Qdot 800, PE-Cy 7, PE-Alexa Fluor 750

The Invitrogen Attune NxT Flow Cytometer was developed with acoustic-assisted hydrodynamic focusing technology, and advanced fluidics designed to minimize clogging and effectively handle a broad range of cell types. This allows for a higher degree of data, detail, and throughput that enables processing of a large range of sample types, including large clumpy cells, samples with a low concentration of cells, and precious samples, more quickly and accurately than ever before with no loss in data quality.

The following applications were performed using the Attune NxT Flow Cytometer. 


Literature


Manuals



Cell types successfully analyzed on the Attune NxT Flow Cytometer

Sample type Demonstrated example Published References
Algae Nannochloropsis oculata  
Bacteria Escherichia coli
Bacillus subtilis
Legionella strains
Staphyloccus aureus  
Diatom Phaeodactylum tricornutum
Human Human adenocarcinoma
Human bone marrow (PBMCs)
Human bone osteosarcoma cells (U2OS)
Human cardiomyocytes  
Human corneal epithelial cells  
Human embryonic kidney (293FT)
Human endothelial cells
Human leukocytes  
Human mesenchymal stem cell (hMSC)
Human red blood cells (RBC)  
Human T cell leukemia (Jurkat)
Human whole blood
Mouse Mouse splenocytes
Mouse T and B regulatory cells
Mouse cardiomyocytes
Mouse bone marrow
Mouse lung tissue
Mouse tumor cells
Mouse skeletal muscle
Parasites Plasmodium falciparum
L. amazonensis, T. brucei and T. cruzi
Trypanosoma cruzi
Picophytoplankton Prochlorococcus spp;
Synechococcus spp
 
Plants Arabidopsis thaliana  
Phalaris arundinacea
Solidago canadensis
Yeast Candida albicans
Saccharomyces cerevisiae
Zebra fish Zebra fish organs

On-site service plans at a glance

Increase your lab productivity and uptime, enabling you to focus on what matters most, your research success.

Product AB Complete AB Assurance AB Maintenance
Standard on-site response time Next business day* 2 business days* 3 business days**
Scheduled on-site PM      
Remote instrument diagnostics      
Priority access to technical support
(Monday–Friday, standard business hours)
     
Parts, labor, and travel for repair      
Priority access to remote service engineer      
Post-PM requalification and critical repairs      
Field applications scientist (FAS) consultation      

* Availability limited in some geographic areas.
** After receipt of a purchase order.

Request a quote


Planned maintenance

Planned Maintenance service for the Attune NxT Flow Cytometer includes:

Checks completed during planned maintenance visit:
System maintenance
  • visually inspect all cables
  • clean the interior and the exterior of unit
  • clean the cove tray and visor
  • clean the tube lifter
  • check the tube lifter for proper operation
Computer maintenance
  • run the disk cleanup
  • defragment the disk, only if the operating system indicates that it is recommended
Fluidics system maintenance
  • replace the SIP tube
  • inspect the instrument internally for any traces of salt residue
  • check for internal leaks and damage at all fittings, valves, pumps and syringe pump
  • replace the 1 mL syringe
  • run the system decontamination script, if not previously done by the customer
  • replace both 0.2 micron filters, if not previously done by the customer
Optics maintenance
  • inspect all optical filters. Clean only if necessary
  • verify the laser alignment, including the pinholes and blocker bar
System diagnostics
  • run the System Test and ensure that everything passes
  • ensure that the latest Attune NxT Software version is installed
  • check leak detector functionality
  • record laser hours
Attune NxT Autosampler maintenance (if applicable to unit)
  • run the Attune NxT Autosampler calibration script
  • replace syringe
Instrument performance
  • ensure that the baseline and performance tests pass the specifications

Compliance services

Get timely, cost-effective, and audit-ready documentation managed by a compliance specialist to help ensure your Attune NxT Flow Cytometer system is installed, operating, and performing to the manufacturer’s specifications.

Verify instrument performance and minimize downtime with IQ/OQ/IPV Services

Our factory-trained and experienced engineers will conduct Installation Qualification (IQ), Operational Qualification (OQ), and Instrument Performance Verification (IPV) on the Invitrogen Attune NxT Flow Cytometer system in your lab. Documentation for audits is provided.

Find compliance documents for Installation and Operational Qualification (IQ/OQ), and Instrument Verification (IPV) for your systems


Technical & Application Support

Take the hassle out of your hands with custom solutions

Assay development & labeling services—our custom solutions to help develop the most appropriate assay for your research or discovery target of interest.

Custom antibodies—our custom antibody development, purification, and screening services deliver the quality, consistency, and performance you need.


Education services

Application and instrument training programs are available at our training centers located throughout the world, within your lab, or through web-based instruction. We offer a wide range of courses to build the skills of cellular analysis professionals and users of the Attune NxT Flow Cytometer:

  • Training Center Courses—get away from the day-to-day responsibilities of the lab and dedicate time to focus on your education needs. Courses are hosted at Thermo Fisher Scientific Training Center sites to provide professional, interactive education that includes classroom instruction and hands-on training in the laboratory.
  • Customer Site/content—courses are offered at your location by a Field Application Scientist (FAS) to provide professional, interactive education that includes hands-on instruction in your laboratory.
  • LabCoat Live—learn a specific application in as little as 1 week. Courses provide interactive education via online lectures and a self-paced hands-on experiment using provided protocols and reagents that we ship to your lab.
  • eLearning—discover new areas of interest and learn about life science topics at your own pace; courses provide education via on-demand, pre-recorded webinars.

Learn more about our Flow Cytometry Webinars
Take our T Cell Stimulation and Proliferation eLearning Course


Instrument management

Manage your instrument use and care online with Instrument Management. Whether you are responsible for one instrument or hundreds of instruments, Instrument Management enables you to easily manage your instrument use and care online.

  • 24/7 access to complete instrument service history and field service reports
  • Request online service and service contract quotes
  • Instrument software, patches, and updates
  • Online instrument scheduler for instrument availability
Online instrument scheduler for instrument availability

Find out more about Instrument Management at thermofisher.com/easiertomanage

3D Demo: Explore the Attune NxT Flow Cytometer and Autosampler

A software tool for exploring the hardware, software, optics and fluidics of the Attune NxT Flow Cytometer. Hot spots throughout the tool feature data, researcher opinions, and technical specifications. Mouse operation enables rotation, zoom, and measurement. Available in 8 languages.

Service plans

Optimize your instrument performance, maximize the availability of your critical systems, and streamline your laboratory workflows. Choose the level of support for your Attune NxT Flow Cytometer that best meets your laboratory's needs and budget:

Consumables and accessories

In addition to accessories such as sample syringes, find the Invitrogen Attune solutions necessary to operate your Attune NxT Flow Cytometer including:

  • Attune Focusing Fluid
  • Attune Wash Solution
  • Attune Shutdown Solution
  • Attune Performance Tracking Beads