2019 Attune NxT Flow Cytometer
Clogging has met its match
- Minimize the hassle, frustration, and sample-loss associated with a clogged instrument
- Investigate difficult samples and large, clumpy cells, including cancer cells, cardiomyocytes, and adipocytes, which were previously not amenable to flow cytometry
Efficient. Flexible. Transformative.
The 2018 Attune NxT Flow Cytometer system makes multiparametric flow cytometry available to both new and experienced researchers. Experience the difference acoustic assisted hydrodynamic focusing technology brings to your workflow. Designed for faster experimental run times, this compact flow cytometer offers proven, rapid and accurate analysis for a broad range of sample types.
- Efficient—up to 10X faster than traditional flow cytometric analyzers
- Flexible—up to 14 colors; up to 6 channels off the violet laser
- Transformative—run difficult samples with a system that is less sensitive to clogging
A flow cytometer designed with your research in mind
- Conserve sample and quickly run experiments with sample flow rates ranging from 12.5–1,000 μL/min
- Intuitive software interface and easy-to-follow workflow that is quick to learn, teach and use
- Save time and minimize cell handling with faster protocols
- Expand your capabilities with up to 6 channels of detection off the violet laser
Invitrogen Attune NxT Flow Cytometer Demo Video
Designed for faster experimental run times, the Invitrogen Attune NxT compact flow cytometer offers rapid and accurate analysis for a broad range of sample types. Experience the difference.
Investigating flow cytometry instrument capabilities to maximize your research
The 40-page Flow Cytometer Evaluation Guide will enable a better understanding of the components and capabilities of various flow cytometers for objective comparison of instruments from several manufacturers.
Freedom of choice
- Option for up to 6 channels off your violet laser with the Attune NxT Violet Laser Upgrade Kit
- Configurable laser and filters provide more choices when designing multicolor panels
- Broaden your capabilities with field upgradeability of up to 4 lasers and 14 colors
- Expand multiparameter capabilities with detection of dim to very bright signals
- See compatible reagents by instrument configuration or detection channel
An evolutionary flow cytometer
- Acquire sufficient events even from very dilute samples with acoustic-assisted hydrodynamic focusing
- Investigate difficult sample types without fear of clogging
- Protect precious samples and boost success in rare-cell experiments (like this researcher discovered)
Automation for any lab
Improve your workflow for fully automated sample acquisition from 96-well and 384-well microtiter plates. There are no physical changes to the system needed in order to run plate based experiments—everything is controlled in the software.
- Attune Autosampler is compact and compatible with many different types of plates
- No carousel needed; run deep well plates up to 2 mL
- Rapid processing of up to 384 samples
2019 Attune NxT instrument options
This grow-with-you system can be customized with up to 4 lasers and 14 colors and now up to 6 channels off of the violet laser. The optical system was designed to provide optimal signal and has the range to detect very bright as well as dim signals. The Attune NxT Flow Cytometer allows researchers the freedom to build multicolor panels, accommodating common fluorophores and dyes. Build your multicolor panel for the Attune NxT flow cytometer with our superior reagents and antibodies.
Attune NxT Flow Cytometer configuration options
Lasers | Laser configuration (Cat. No.) |
Lasers included, No. of detection channels | Total detection channels* | ||||
---|---|---|---|---|---|---|---|
Violet, 405 nm | Blue, 488 nm | Yellow, 561 nm | Green, 532 nm |
Red, 637 nm | |||
1 | Blue (A24864) |
Available as upgrade† |
4
|
Available as upgrade† | Available as upgrade† | Available as upgrade† | 6 |
2 | Blue/green (A28995) |
Available as upgrade† |
3
|
4
|
Available as upgrade† | 9 | |
Blue/yellow (A24861) |
Available as upgrade† |
3
|
4
|
Available as upgrade† | 9 | ||
Blue/red (A24863) |
Available as upgrade† |
4
|
Available as upgrade† | Available as upgrade† |
3
|
9 | |
Blue/violet (A24862) |
4
|
4
|
Available as upgrade† | Available as upgrade† | Available as upgrade† | 10 | |
Blue/violet 6 (A29002) |
6
|
3
|
Available as upgrade† | Available as upgrade† | 11 | ||
3 | Blue/green/red (A28997) |
Available as upgrade†
|
3
|
4
|
3
|
12 | |
Blue/red/yellow (A28993) | Available as upgrade† | 3 | 4 | 3 | 12 | ||
Blue/green/violet (A28999) |
4
|
3
|
4
|
Available as upgrade† | 13 | ||
Blue/violet/yellow (A24859) |
4
|
3
|
4
|
|
Available as upgrade† | 13 | |
Blue/red/violet (A24860) |
4
|
4
|
Available as upgrade† | Available as upgrade† |
3
|
13 | |
Blue/red/violet 6 (A29003) |
6
|
3
|
Available as upgrade† |
3
|
14 | ||
4 | Blue/red/violet/green (A29001) |
4
|
3
|
4
|
3
|
16 | |
Blue/red/yellow/violet (A24858) |
4
|
3
|
4
|
3
|
16 | ||
Blue/red/yellow/violet 6 (A29004) |
6
|
2
|
3
|
3
|
16 | ||
* Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel. † Attune NxT Flow Cytometer owners wishing to purchase a laser upgrade should request a quote here: Yellow Laser Upgrade, Violet Laser Upgrade, Red Laser Upgrade, or Green Laser Upgrade. After we receive your quote request, a technical sales specialist will contact you about upgrade options. |
Are you comparing the specifications of more than one flow cytometer?
A specification is derived from a specific test or calculation, but these tests aren’t standardized across instrument developers and may be misleading in a side-by-side comparison because their values may have been calculated in different ways, despite sharing the same terminology. This 8-page Comparing Technical Specification Documents resource offers helpful tips on deciphering variations.
Optics |
|
||||||||||||||||||||||||||||
Fluidics |
|
||||||||||||||||||||||||||||
Performance |
|
||||||||||||||||||||||||||||
Software |
|
||||||||||||||||||||||||||||
Quality and regulatory |
|
||||||||||||||||||||||||||||
Data management |
|
||||||||||||||||||||||||||||
Installation requirements |
|
Simple yet powerful software, easy auto compensation
Invitrogen Attune NxT Software is designed to provide powerful data acquisition and analysis using an intuitive, user-friendly interface. The instrument software features and benefits include:
- Compensation—fully automated and manual compensation
- Instrument tracking—automated baseline and performance test for all channels and linearity
- Automated maintenance—startup (<15 min), shutdown, rinse, wash, debubble, deep clean,
monthly decontamination - User account maintenance—administrative and individual accounts with user log
- Gates—standard and customizable gates
- Plot previews—easy workspace setup
- Zoom filmstrip viewer—easy visualization and navigation of plots to set gates and plot attributes
- File formats—FCS 3.0 and FCS 3.1
- Graphics resolution—publication-quality images (customizable)
Transforming capabilities
Since its initial unveiling, the compact Attune NxT Flow Cytometer continues to offer more options and added functionality with additional lasers, more detection channels, increased flexibility, and design modifications that further improve the performance, reliability, and robustness.
Find laser upgrade and conversion kits to expand the capabilities of your instrument, as well as filter kits and accessory parts.
Attune NxT upgrades | Filter | Common fluorochromes | Cat. No. |
---|---|---|---|
Attune NxT Yellow Laser Upgrade Kit | 585/16 | PE, Alexa Fluor 532, Alexa Fluor 568 | 100022779 |
620/15 | Alexa Fluor 594, PE-Texas Red, PE-Alexa Fluor 610 | ||
695/40 | PE-Alexa Fluor 700, TRI-COLOR (PE-Cy5), PE-Cy5.5, Qdot 705 | ||
780/60 | Qdot 800, PE-Cy 7, PE-Alexa Fluor 750 | ||
Attune NxT Red Laser Upgrade Kit | 670/14 | APC, Alexa Fluor 647, Qdot 655 | 100022778 |
720/30 | Alexa Fluor 680, Alexa Fluor 700, APC-Alexa Fluor 700, Qdot 705 | ||
780/60 | APC-Alexa Fluor 750, APC-Cy7, APC-eFluor 780, Qdot 800 | ||
Attune NxT Green Laser Upgrade Kit | 575/36 | PE, Alexa Fluor 532, Alexa Fluor 555, DsRed | A32701 |
620/15 | Alexa Fluor 568, Alexa Fluor 594, Qdot 605, PE-Texas Red, PE-Alexa Fluor 610 | ||
695/40 | Qdot 705, PE-Alexa Fluor 700, TRI-COLOR, PE-Cy5.5 | ||
780/60 | Qdot 800, PE-Cy7 | ||
Attune NxT Violet Laser Upgrade Kit | 440/50 | Super Bright 436, Alexa Fluor 405, eFluor 450, Pacific Blue | 100022777 |
512/25 | eFluor 506, Pacific Green | ||
603/48 | Super Bight 600, Pacific Orange, Qdot 605 | ||
710/50 | Super Bright 702, Qdot 705 | ||
Attune NxT Violet 6 Conversion Kit, Blue Laser | 530/30 | Alexa Fluor 488, FITC | A35428 |
574/26 | PE, PE-Alexa Fluor 610, Alexa Fluor 546 | ||
695/40 | PE-Alexa Fluor 700, PE-Cy5.5, PerCP, PerCP-Cy5.5, Qdot 705, TRI-COLOR | ||
Attune NxT Violet 6 Conversion Kit, Violet Laser | 450/40 | Super Bright 436, eFluor 450, Pacific Blue | A36569 |
525/50 | eFluor 506, Pacific Green, eFluor 506 | ||
610/20 | Super Bright 600, Qdot 605, Pacific Orange | ||
660/20 | Super Bright 645 | ||
710/50 | Super Bright 702, Qdot 705 | ||
780/60 | Super Bright 780 | ||
Attune NxT Violet 6 Conversion Kit, Red Laser | 670/14 | APC, Alexa Fluor 647, Qdot 655 | A36571 |
720/30 | Alexa Fluor 680, Alexa Fluor 700, APC-Alexa Fluor 700, Qdot 705 | ||
780/60 | APC-Alexa Fluor 750, APC-eFluor 780, APC-Cy7, Qdot 800 | ||
Attune NxT Violet 6 Conversion Kit, Yellow Laser | 585/16 | PE, Alexa Fluor 532, Alexa Fluor 568 | A36572 |
620/15 | Alexa Fluor 594, PE-Texas Red, PE-Alexa Fluor 610 | ||
695/40 | PE-Alexa Fluor 700, TRI-COLOR, PE-Cy5.5, Qdot 705 | ||
780/60 | Qdot 800, PE-Cy 7, PE-Alexa Fluor 750 |
The Invitrogen Attune NxT Flow Cytometer was developed with acoustic-assisted hydrodynamic focusing technology, and advanced fluidics designed to minimize clogging and effectively handle a broad range of cell types. This allows for a higher degree of data, detail, and throughput that enables processing of a large range of sample types, including large clumpy cells, samples with a low concentration of cells, and precious samples, more quickly and accurately than ever before with no loss in data quality.
The following applications were performed using the Attune NxT Flow Cytometer.
Immunophenotyping
- Validated 10-color T cell panel using the Attune NxT Flow Cytometer
- Attune NxT Acoustic Focusing Cytometer for 6-color immunophenotyping analysis of stained human whole blood using a no-lyse, no-wash protocol, with no compensation
- Ten-parameter immunophenotyping of human lysed whole blood with the Attune NxT Acoustic Focusing Cytometer allows for the identification of multiple T cell subsets and myeloid cells
- 13-parameter immunophenotyping of human lysed whole blood with the the Attune NxT Flow Cytometer allows for the identification of B cells, NK cells, multiple T cell subsets and myeloid cells
- Multiparameter analysis of murine regulatory T cells and dendritic cells with the Attune NxT Acoustic Focusing Cytometer
- Detection of murine regulatory T cells on the Attune NxT Acoustic Focusing Cytometer
No-wash, no lyse applications
- Detection of platelets in whole blood using the Attune NxT Flow Cytometer
- No-wash, no-lyse detection of leukocytes in human whole blood on the Attune NxT Acoustic Focusing Cytometer
- No-wash, no-lyse detection of phagocytic cells via a pHrodo BioParticles functional assay in human whole blood on the Attune NxT Acoustic Focusing Cytometer
Plants
Fluorescent protein detection
Stem cells
Literature
Manuals
Posters
Cell types successfully analyzed on the Attune NxT Flow Cytometer
Sample type | Demonstrated example | Published References |
---|---|---|
Algae | Nannochloropsis oculata | |
Bacteria | Escherichia coli | |
Bacillus subtilis | ||
Legionella strains | ||
Staphyloccus aureus | ||
Diatom | Phaeodactylum tricornutum | |
Human | Human adenocarcinoma | |
Human bone marrow (PBMCs) | ||
Human bone osteosarcoma cells (U2OS) | ||
Human cardiomyocytes | ||
Human corneal epithelial cells | ||
Human embryonic kidney (293FT) | ||
Human endothelial cells | ||
Human leukocytes | ||
Human mesenchymal stem cell (hMSC) | ||
Human red blood cells (RBC) | ||
Human T cell leukemia (Jurkat) | ||
Human whole blood | ||
Mouse | Mouse splenocytes | |
Mouse T and B regulatory cells | ||
Mouse cardiomyocytes | ||
Mouse bone marrow | ||
Mouse lung tissue | ||
Mouse tumor cells | ||
Mouse skeletal muscle | ||
Parasites | Plasmodium falciparum | |
L. amazonensis, T. brucei and T. cruzi | ||
Trypanosoma cruzi | ||
Picophytoplankton | Prochlorococcus spp; Synechococcus spp |
|
Plants | Arabidopsis thaliana | |
Phalaris arundinacea | ||
Solidago canadensis | ||
Yeast | Candida albicans | |
Saccharomyces cerevisiae | ||
Zebra fish | Zebra fish organs |
On-site service plans at a glance
Increase your lab productivity and uptime, enabling you to focus on what matters most, your research success.
Product | AB Complete | AB Assurance | AB Maintenance |
---|---|---|---|
Standard on-site response time | Next business day* | 2 business days* | 3 business days** |
Scheduled on-site PM | |||
Remote instrument diagnostics | |||
Priority access to technical support (Monday–Friday, standard business hours) |
|||
Parts, labor, and travel for repair | |||
Priority access to remote service engineer | |||
Post-PM requalification and critical repairs | |||
Field applications scientist (FAS) consultation |
* Availability limited in some geographic areas.
** After receipt of a purchase order.
Planned maintenance
Planned Maintenance service for the Attune NxT Flow Cytometer includes:
- Performance assurance checks
- Functionality and safety checks
- Safety inspections in accordance with all applicable regulations
- Interior and exterior cleaning of the instrument
- Components check up
- Software update
- Routine inspection and replacement of the following:
- Updates relating to all service bulletins relevant to instrument configuration
Checks completed during planned maintenance visit: | |
---|---|
System maintenance |
|
Computer maintenance |
|
Fluidics system maintenance |
|
Optics maintenance |
|
System diagnostics |
|
Attune NxT Autosampler maintenance (if applicable to unit) |
|
Instrument performance |
|
Compliance services
Get timely, cost-effective, and audit-ready documentation managed by a compliance specialist to help ensure your Attune NxT Flow Cytometer system is installed, operating, and performing to the manufacturer’s specifications.
Verify instrument performance and minimize downtime with IQ/OQ/IPV Services
Our factory-trained and experienced engineers will conduct Installation Qualification (IQ), Operational Qualification (OQ), and Instrument Performance Verification (IPV) on the Invitrogen Attune NxT Flow Cytometer system in your lab. Documentation for audits is provided.
Technical & Application Support
Take the hassle out of your hands with custom solutions
Assay development & labeling services—our custom solutions to help develop the most appropriate assay for your research or discovery target of interest.
Custom antibodies—our custom antibody development, purification, and screening services deliver the quality, consistency, and performance you need.
Education services
Application and instrument training programs are available at our training centers located throughout the world, within your lab, or through web-based instruction. We offer a wide range of courses to build the skills of cellular analysis professionals and users of the Attune NxT Flow Cytometer:
- Training Center Courses—get away from the day-to-day responsibilities of the lab and dedicate time to focus on your education needs. Courses are hosted at Thermo Fisher Scientific Training Center sites to provide professional, interactive education that includes classroom instruction and hands-on training in the laboratory.
- Customer Site/content—courses are offered at your location by a Field Application Scientist (FAS) to provide professional, interactive education that includes hands-on instruction in your laboratory.
- LabCoat Live—learn a specific application in as little as 1 week. Courses provide interactive education via online lectures and a self-paced hands-on experiment using provided protocols and reagents that we ship to your lab.
- eLearning—discover new areas of interest and learn about life science topics at your own pace; courses provide education via on-demand, pre-recorded webinars.
Learn more about our Flow Cytometry Webinars
Take our T Cell Stimulation and Proliferation eLearning Course
Instrument management
Manage your instrument use and care online with Instrument Management. Whether you are responsible for one instrument or hundreds of instruments, Instrument Management enables you to easily manage your instrument use and care online.
- 24/7 access to complete instrument service history and field service reports
- Request online service and service contract quotes
- Instrument software, patches, and updates
- Online instrument scheduler for instrument availability
Find out more about Instrument Management at thermofisher.com/easiertomanage
A flow cytometer designed with your research in mind
- Conserve sample and quickly run experiments with sample flow rates ranging from 12.5–1,000 μL/min
- Intuitive software interface and easy-to-follow workflow that is quick to learn, teach and use
- Save time and minimize cell handling with faster protocols
- Expand your capabilities with up to 6 channels of detection off the violet laser
Invitrogen Attune NxT Flow Cytometer Demo Video
Designed for faster experimental run times, the Invitrogen Attune NxT compact flow cytometer offers rapid and accurate analysis for a broad range of sample types. Experience the difference.
Investigating flow cytometry instrument capabilities to maximize your research
The 40-page Flow Cytometer Evaluation Guide will enable a better understanding of the components and capabilities of various flow cytometers for objective comparison of instruments from several manufacturers.
Freedom of choice
- Option for up to 6 channels off your violet laser with the Attune NxT Violet Laser Upgrade Kit
- Configurable laser and filters provide more choices when designing multicolor panels
- Broaden your capabilities with field upgradeability of up to 4 lasers and 14 colors
- Expand multiparameter capabilities with detection of dim to very bright signals
- See compatible reagents by instrument configuration or detection channel
An evolutionary flow cytometer
- Acquire sufficient events even from very dilute samples with acoustic-assisted hydrodynamic focusing
- Investigate difficult sample types without fear of clogging
- Protect precious samples and boost success in rare-cell experiments (like this researcher discovered)
Automation for any lab
Improve your workflow for fully automated sample acquisition from 96-well and 384-well microtiter plates. There are no physical changes to the system needed in order to run plate based experiments—everything is controlled in the software.
- Attune Autosampler is compact and compatible with many different types of plates
- No carousel needed; run deep well plates up to 2 mL
- Rapid processing of up to 384 samples
2019 Attune NxT instrument options
This grow-with-you system can be customized with up to 4 lasers and 14 colors and now up to 6 channels off of the violet laser. The optical system was designed to provide optimal signal and has the range to detect very bright as well as dim signals. The Attune NxT Flow Cytometer allows researchers the freedom to build multicolor panels, accommodating common fluorophores and dyes. Build your multicolor panel for the Attune NxT flow cytometer with our superior reagents and antibodies.
Attune NxT Flow Cytometer configuration options
Lasers | Laser configuration (Cat. No.) |
Lasers included, No. of detection channels | Total detection channels* | ||||
---|---|---|---|---|---|---|---|
Violet, 405 nm | Blue, 488 nm | Yellow, 561 nm | Green, 532 nm |
Red, 637 nm | |||
1 | Blue (A24864) |
Available as upgrade† |
4
|
Available as upgrade† | Available as upgrade† | Available as upgrade† | 6 |
2 | Blue/green (A28995) |
Available as upgrade† |
3
|
4
|
Available as upgrade† | 9 | |
Blue/yellow (A24861) |
Available as upgrade† |
3
|
4
|
Available as upgrade† | 9 | ||
Blue/red (A24863) |
Available as upgrade† |
4
|
Available as upgrade† | Available as upgrade† |
3
|
9 | |
Blue/violet (A24862) |
4
|
4
|
Available as upgrade† | Available as upgrade† | Available as upgrade† | 10 | |
Blue/violet 6 (A29002) |
6
|
3
|
Available as upgrade† | Available as upgrade† | 11 | ||
3 | Blue/green/red (A28997) |
Available as upgrade†
|
3
|
4
|
3
|
12 | |
Blue/red/yellow (A28993) | Available as upgrade† | 3 | 4 | 3 | 12 | ||
Blue/green/violet (A28999) |
4
|
3
|
4
|
Available as upgrade† | 13 | ||
Blue/violet/yellow (A24859) |
4
|
3
|
4
|
|
Available as upgrade† | 13 | |
Blue/red/violet (A24860) |
4
|
4
|
Available as upgrade† | Available as upgrade† |
3
|
13 | |
Blue/red/violet 6 (A29003) |
6
|
3
|
Available as upgrade† |
3
|
14 | ||
4 | Blue/red/violet/green (A29001) |
4
|
3
|
4
|
3
|
16 | |
Blue/red/yellow/violet (A24858) |
4
|
3
|
4
|
3
|
16 | ||
Blue/red/yellow/violet 6 (A29004) |
6
|
2
|
3
|
3
|
16 | ||
* Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel. † Attune NxT Flow Cytometer owners wishing to purchase a laser upgrade should request a quote here: Yellow Laser Upgrade, Violet Laser Upgrade, Red Laser Upgrade, or Green Laser Upgrade. After we receive your quote request, a technical sales specialist will contact you about upgrade options. |
Are you comparing the specifications of more than one flow cytometer?
A specification is derived from a specific test or calculation, but these tests aren’t standardized across instrument developers and may be misleading in a side-by-side comparison because their values may have been calculated in different ways, despite sharing the same terminology. This 8-page Comparing Technical Specification Documents resource offers helpful tips on deciphering variations.
Optics |
|
||||||||||||||||||||||||||||
Fluidics |
|
||||||||||||||||||||||||||||
Performance |
|
||||||||||||||||||||||||||||
Software |
|
||||||||||||||||||||||||||||
Quality and regulatory |
|
||||||||||||||||||||||||||||
Data management |
|
||||||||||||||||||||||||||||
Installation requirements |
|
Simple yet powerful software, easy auto compensation
Invitrogen Attune NxT Software is designed to provide powerful data acquisition and analysis using an intuitive, user-friendly interface. The instrument software features and benefits include:
- Compensation—fully automated and manual compensation
- Instrument tracking—automated baseline and performance test for all channels and linearity
- Automated maintenance—startup (<15 min), shutdown, rinse, wash, debubble, deep clean,
monthly decontamination - User account maintenance—administrative and individual accounts with user log
- Gates—standard and customizable gates
- Plot previews—easy workspace setup
- Zoom filmstrip viewer—easy visualization and navigation of plots to set gates and plot attributes
- File formats—FCS 3.0 and FCS 3.1
- Graphics resolution—publication-quality images (customizable)
Transforming capabilities
Since its initial unveiling, the compact Attune NxT Flow Cytometer continues to offer more options and added functionality with additional lasers, more detection channels, increased flexibility, and design modifications that further improve the performance, reliability, and robustness.
Find laser upgrade and conversion kits to expand the capabilities of your instrument, as well as filter kits and accessory parts.
Attune NxT upgrades | Filter | Common fluorochromes | Cat. No. |
---|---|---|---|
Attune NxT Yellow Laser Upgrade Kit | 585/16 | PE, Alexa Fluor 532, Alexa Fluor 568 | 100022779 |
620/15 | Alexa Fluor 594, PE-Texas Red, PE-Alexa Fluor 610 | ||
695/40 | PE-Alexa Fluor 700, TRI-COLOR (PE-Cy5), PE-Cy5.5, Qdot 705 | ||
780/60 | Qdot 800, PE-Cy 7, PE-Alexa Fluor 750 | ||
Attune NxT Red Laser Upgrade Kit | 670/14 | APC, Alexa Fluor 647, Qdot 655 | 100022778 |
720/30 | Alexa Fluor 680, Alexa Fluor 700, APC-Alexa Fluor 700, Qdot 705 | ||
780/60 | APC-Alexa Fluor 750, APC-Cy7, APC-eFluor 780, Qdot 800 | ||
Attune NxT Green Laser Upgrade Kit | 575/36 | PE, Alexa Fluor 532, Alexa Fluor 555, DsRed | A32701 |
620/15 | Alexa Fluor 568, Alexa Fluor 594, Qdot 605, PE-Texas Red, PE-Alexa Fluor 610 | ||
695/40 | Qdot 705, PE-Alexa Fluor 700, TRI-COLOR, PE-Cy5.5 | ||
780/60 | Qdot 800, PE-Cy7 | ||
Attune NxT Violet Laser Upgrade Kit | 440/50 | Super Bright 436, Alexa Fluor 405, eFluor 450, Pacific Blue | 100022777 |
512/25 | eFluor 506, Pacific Green | ||
603/48 | Super Bight 600, Pacific Orange, Qdot 605 | ||
710/50 | Super Bright 702, Qdot 705 | ||
Attune NxT Violet 6 Conversion Kit, Blue Laser | 530/30 | Alexa Fluor 488, FITC | A35428 |
574/26 | PE, PE-Alexa Fluor 610, Alexa Fluor 546 | ||
695/40 | PE-Alexa Fluor 700, PE-Cy5.5, PerCP, PerCP-Cy5.5, Qdot 705, TRI-COLOR | ||
Attune NxT Violet 6 Conversion Kit, Violet Laser | 450/40 | Super Bright 436, eFluor 450, Pacific Blue | A36569 |
525/50 | eFluor 506, Pacific Green, eFluor 506 | ||
610/20 | Super Bright 600, Qdot 605, Pacific Orange | ||
660/20 | Super Bright 645 | ||
710/50 | Super Bright 702, Qdot 705 | ||
780/60 | Super Bright 780 | ||
Attune NxT Violet 6 Conversion Kit, Red Laser | 670/14 | APC, Alexa Fluor 647, Qdot 655 | A36571 |
720/30 | Alexa Fluor 680, Alexa Fluor 700, APC-Alexa Fluor 700, Qdot 705 | ||
780/60 | APC-Alexa Fluor 750, APC-eFluor 780, APC-Cy7, Qdot 800 | ||
Attune NxT Violet 6 Conversion Kit, Yellow Laser | 585/16 | PE, Alexa Fluor 532, Alexa Fluor 568 | A36572 |
620/15 | Alexa Fluor 594, PE-Texas Red, PE-Alexa Fluor 610 | ||
695/40 | PE-Alexa Fluor 700, TRI-COLOR, PE-Cy5.5, Qdot 705 | ||
780/60 | Qdot 800, PE-Cy 7, PE-Alexa Fluor 750 |
The Invitrogen Attune NxT Flow Cytometer was developed with acoustic-assisted hydrodynamic focusing technology, and advanced fluidics designed to minimize clogging and effectively handle a broad range of cell types. This allows for a higher degree of data, detail, and throughput that enables processing of a large range of sample types, including large clumpy cells, samples with a low concentration of cells, and precious samples, more quickly and accurately than ever before with no loss in data quality.
The following applications were performed using the Attune NxT Flow Cytometer.
Immunophenotyping
- Validated 10-color T cell panel using the Attune NxT Flow Cytometer
- Attune NxT Acoustic Focusing Cytometer for 6-color immunophenotyping analysis of stained human whole blood using a no-lyse, no-wash protocol, with no compensation
- Ten-parameter immunophenotyping of human lysed whole blood with the Attune NxT Acoustic Focusing Cytometer allows for the identification of multiple T cell subsets and myeloid cells
- 13-parameter immunophenotyping of human lysed whole blood with the the Attune NxT Flow Cytometer allows for the identification of B cells, NK cells, multiple T cell subsets and myeloid cells
- Multiparameter analysis of murine regulatory T cells and dendritic cells with the Attune NxT Acoustic Focusing Cytometer
- Detection of murine regulatory T cells on the Attune NxT Acoustic Focusing Cytometer
No-wash, no lyse applications
- Detection of platelets in whole blood using the Attune NxT Flow Cytometer
- No-wash, no-lyse detection of leukocytes in human whole blood on the Attune NxT Acoustic Focusing Cytometer
- No-wash, no-lyse detection of phagocytic cells via a pHrodo BioParticles functional assay in human whole blood on the Attune NxT Acoustic Focusing Cytometer
Plants
Fluorescent protein detection
Stem cells
Literature
Manuals
Posters
Cell types successfully analyzed on the Attune NxT Flow Cytometer
Sample type | Demonstrated example | Published References |
---|---|---|
Algae | Nannochloropsis oculata | |
Bacteria | Escherichia coli | |
Bacillus subtilis | ||
Legionella strains | ||
Staphyloccus aureus | ||
Diatom | Phaeodactylum tricornutum | |
Human | Human adenocarcinoma | |
Human bone marrow (PBMCs) | ||
Human bone osteosarcoma cells (U2OS) | ||
Human cardiomyocytes | ||
Human corneal epithelial cells | ||
Human embryonic kidney (293FT) | ||
Human endothelial cells | ||
Human leukocytes | ||
Human mesenchymal stem cell (hMSC) | ||
Human red blood cells (RBC) | ||
Human T cell leukemia (Jurkat) | ||
Human whole blood | ||
Mouse | Mouse splenocytes | |
Mouse T and B regulatory cells | ||
Mouse cardiomyocytes | ||
Mouse bone marrow | ||
Mouse lung tissue | ||
Mouse tumor cells | ||
Mouse skeletal muscle | ||
Parasites | Plasmodium falciparum | |
L. amazonensis, T. brucei and T. cruzi | ||
Trypanosoma cruzi | ||
Picophytoplankton | Prochlorococcus spp; Synechococcus spp |
|
Plants | Arabidopsis thaliana | |
Phalaris arundinacea | ||
Solidago canadensis | ||
Yeast | Candida albicans | |
Saccharomyces cerevisiae | ||
Zebra fish | Zebra fish organs |
On-site service plans at a glance
Increase your lab productivity and uptime, enabling you to focus on what matters most, your research success.
Product | AB Complete | AB Assurance | AB Maintenance |
---|---|---|---|
Standard on-site response time | Next business day* | 2 business days* | 3 business days** |
Scheduled on-site PM | |||
Remote instrument diagnostics | |||
Priority access to technical support (Monday–Friday, standard business hours) |
|||
Parts, labor, and travel for repair | |||
Priority access to remote service engineer | |||
Post-PM requalification and critical repairs | |||
Field applications scientist (FAS) consultation |
* Availability limited in some geographic areas.
** After receipt of a purchase order.
Planned maintenance
Planned Maintenance service for the Attune NxT Flow Cytometer includes:
- Performance assurance checks
- Functionality and safety checks
- Safety inspections in accordance with all applicable regulations
- Interior and exterior cleaning of the instrument
- Components check up
- Software update
- Routine inspection and replacement of the following:
- Updates relating to all service bulletins relevant to instrument configuration
Checks completed during planned maintenance visit: | |
---|---|
System maintenance |
|
Computer maintenance |
|
Fluidics system maintenance |
|
Optics maintenance |
|
System diagnostics |
|
Attune NxT Autosampler maintenance (if applicable to unit) |
|
Instrument performance |
|
Compliance services
Get timely, cost-effective, and audit-ready documentation managed by a compliance specialist to help ensure your Attune NxT Flow Cytometer system is installed, operating, and performing to the manufacturer’s specifications.
Verify instrument performance and minimize downtime with IQ/OQ/IPV Services
Our factory-trained and experienced engineers will conduct Installation Qualification (IQ), Operational Qualification (OQ), and Instrument Performance Verification (IPV) on the Invitrogen Attune NxT Flow Cytometer system in your lab. Documentation for audits is provided.
Technical & Application Support
Take the hassle out of your hands with custom solutions
Assay development & labeling services—our custom solutions to help develop the most appropriate assay for your research or discovery target of interest.
Custom antibodies—our custom antibody development, purification, and screening services deliver the quality, consistency, and performance you need.
Education services
Application and instrument training programs are available at our training centers located throughout the world, within your lab, or through web-based instruction. We offer a wide range of courses to build the skills of cellular analysis professionals and users of the Attune NxT Flow Cytometer:
- Training Center Courses—get away from the day-to-day responsibilities of the lab and dedicate time to focus on your education needs. Courses are hosted at Thermo Fisher Scientific Training Center sites to provide professional, interactive education that includes classroom instruction and hands-on training in the laboratory.
- Customer Site/content—courses are offered at your location by a Field Application Scientist (FAS) to provide professional, interactive education that includes hands-on instruction in your laboratory.
- LabCoat Live—learn a specific application in as little as 1 week. Courses provide interactive education via online lectures and a self-paced hands-on experiment using provided protocols and reagents that we ship to your lab.
- eLearning—discover new areas of interest and learn about life science topics at your own pace; courses provide education via on-demand, pre-recorded webinars.
Learn more about our Flow Cytometry Webinars
Take our T Cell Stimulation and Proliferation eLearning Course
Instrument management
Manage your instrument use and care online with Instrument Management. Whether you are responsible for one instrument or hundreds of instruments, Instrument Management enables you to easily manage your instrument use and care online.
- 24/7 access to complete instrument service history and field service reports
- Request online service and service contract quotes
- Instrument software, patches, and updates
- Online instrument scheduler for instrument availability
Find out more about Instrument Management at thermofisher.com/easiertomanage
3D Demo: Explore the Attune NxT Flow Cytometer and Autosampler
A software tool for exploring the hardware, software, optics and fluidics of the Attune NxT Flow Cytometer. Hot spots throughout the tool feature data, researcher opinions, and technical specifications. Mouse operation enables rotation, zoom, and measurement. Available in 8 languages.