2018 Attune NxT Flow Cytometer
Clogging has met its match
- Minimize the hassle, frustration, and sample-loss associated with a clogged instrument
- Investigate difficult samples and large, clumpy cells, including cancer cells, cardiomyocytes, and adipocytes, which were previously not amenable to flow cytometry
Efficient. Flexible. Transformative.
The 2018 Attune NxT Flow Cytometer system makes multiparametric flow cytometry available to both new and experienced researchers. Experience the difference acoustic assisted hydrodynamic focusing technology brings to your workflow. Designed for faster experimental run times, this compact flow cytometer offers proven, rapid and accurate analysis for a broad range of sample types.
- Efficient—up to 10X faster than traditional flow cytometric analyzers
- Flexible—up to 14 colors; up to 6 channels off the violet laser
- Transformative—run difficult samples with a system that is less sensitive to clogging
A flow cytometer designed with your research in mind
- Conserve sample and quickly run experiments with sample flow rates ranging from 12.5–1,000 μL/min
- Intuitive software interface and easy-to-follow workflow that is quick to learn, teach and use
- Save time and minimize cell handling with faster protocols
- Expand your capabilities with up to 6 channels of detection off the violet laser
Investigating flow cytometry instrument capabilities to maximize your research
The 40-page Flow Cytometer Evaluation Guide will enable a better understanding of the components and capabilities of various flow cytometers for objective comparison of instruments from several manufacturers.
Freedom of choice
- Option for up to 6 channels off your violet laser with the Attune NxT Violet Laser Upgrade Kit
- Configurable laser and filters provide more choices when designing multicolor panels
- Broaden your capabilities with field upgradeability of up to 4 lasers and 14 colors
- Expand multiparameter capabilities with detection of dim to very bright signals
- See compatible reagents by instrument configuration or detection channel
An evolutionary flow cytometer
- Acquire sufficient events even from very dilute samples with acoustic-assisted hydrodynamic focusing
- Investigate difficult sample types without fear of clogging
- Protect precious samples and boost success in rare-cell experiments (like this researcher discovered)
Automation for any lab
Improve your workflow for fully automated sample acquisition from 96-well and 384-well microtiter plates. There are no physical changes to the system needed in order to run plate based experiments—everything is controlled in the software.
- Attune Autosampler is compact and compatible with many different types of plates
- No carousel needed; run deep well plates up to 2 mL
- Rapid processing of up to 384 samples
2018 Attune NxT instrument options
This grow-with-you system can be customized with up to 4 lasers and 14 colors and now up to 6 channels off of the violet laser. The optical system was designed to provide optimal signal and has the range to detect very bright as well as dim signals. The Attune NxT Flow Cytometer allows researchers the freedom to build multicolor panels, accommodating common fluorophores and dyes. Build your multicolor panel for the Attune NxT flow cytometer with our superior reagents and antibodies.
Attune NxT Flow Cytometer configuration options
| Lasers | Laser configuration (Cat. No.) |
Lasers included, No. of detection channels | Total detection channels* | ||||
|---|---|---|---|---|---|---|---|
| Violet, 405 nm | Blue, 488 nm | Yellow, 561 nm | Green, 532 nm |
Red, 637 nm | |||
| 1 | Blue (A24864) |
Available as upgrade† |
4
|
Available as upgrade† | Available as upgrade† | Available as upgrade† | 6 |
| 2 | Blue/green (A28995) |
Available as upgrade† |
3
|
4
|
Available as upgrade† | 9 | |
| Blue/yellow (A24861) |
Available as upgrade† |
3
|
4
|
Available as upgrade† | 9 | ||
| Blue/red (A24863) |
Available as upgrade† |
4
|
Available as upgrade† | Available as upgrade† |
3
|
9 | |
| Blue/violet (A24862) |
4
|
4
|
Available as upgrade† | Available as upgrade† | Available as upgrade† | 10 | |
| Blue/violet 6 (A29002) |
6
|
3
|
Available as upgrade† | Available as upgrade† | 11 | ||
| 3 | Blue/green/red (A28997) |
Available as upgrade†
|
3
|
4
|
3
|
12 | |
| Blue/red/yellow (A28993) | Available as upgrade† | 3 | 4 | 3 | 12 | ||
| Blue/green/violet (A28999) |
4
|
3
|
4
|
Available as upgrade† | 13 | ||
| Blue/violet/yellow (A24859) |
4
|
3
|
4
|
|
Available as upgrade† | 13 | |
| Blue/red/violet (A24860) |
4
|
4
|
Available as upgrade† | Available as upgrade† |
3
|
13 | |
| Blue/red/violet 6 (A29003) |
6
|
3
|
Available as upgrade† |
3
|
14 | ||
| 4 | Blue/red/violet/green (A29001) |
4
|
3
|
4
|
3
|
16 | |
| Blue/red/yellow/violet (A24858) |
4
|
3
|
4
|
3
|
16 | ||
| Blue/red/yellow/violet 6 (A29004) |
6
|
2
|
3
|
3
|
16 | ||
| * Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel. † Attune NxT Flow Cytometer owners wishing to purchase a laser upgrade should request a quote here: Yellow Laser Upgrade, Violet Laser Upgrade, Red Laser Upgrade, or Green Laser Upgrade. After we receive your quote request, a technical sales specialist will contact you about upgrade options. |
|||||||
Are you comparing the specifications of more than one flow cytometer?
A specification is derived from a specific test or calculation, but these tests aren’t standardized across instrument developers and may be misleading in a side-by-side comparison because their values may have been calculated in different ways, despite sharing the same terminology. This 8-page Comparing Technical Specification Documents resource offers helpful tips on deciphering variations.
| Optics |
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| Fluidics |
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| Performance |
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| Software |
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| Quality and regulatory |
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| Data management |
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| Installation requirements |
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Simple yet powerful software, easy auto compensation
Invitrogen Attune NxT Software is designed to provide powerful data acquisition and analysis using an intuitive, user-friendly interface. The instrument software features and benefits include:
- Compensation—fully automated and manual compensation
- Instrument tracking—automated baseline and performance test for all channels and linearity
- Automated maintenance—startup (<15 min), shutdown, rinse, wash, debubble, deep clean,
monthly decontamination - User account maintenance—administrative and individual accounts with user log
- Gates—standard and customizable gates
- Plot previews—easy workspace setup
- Zoom filmstrip viewer—easy visualization and navigation of plots to set gates and plot attributes
- File formats—FCS 3.0 and FCS 3.1
- Graphics resolution—publication-quality images (customizable)
Accessories for the Attune Nxt Flow Cytometer
Find laser upgrade and conversion kits to expand the capabilities of your instrument, as well as filter kits and accessory parts.
Meet the Inventor video series
The Invitrogen Attune NxT Flow Cytometer was developed with acoustic-assisted hydrodynamic focusing technology, and advanced fluidics designed to minimize clogging and effectively handle a broad range of cell types. This allows for a higher degree of data, detail, and throughput that enables processing of a large range of sample types, including large clumpy cells, samples with a low concentration of cells, and precious samples, more quickly and accurately than ever before with no loss in data quality.
The following applications were performed using the Attune NxT Flow Cytometer.
Immunophenotyping
- Validated 10-color T cell panel using the Attune NxT Flow Cytometer
- Attune NxT Acoustic Focusing Cytometer for 6-color immunophenotyping analysis of stained human whole blood using a no-lyse, no-wash protocol, with no compensation
- Ten-parameter immunophenotyping of human lysed whole blood with the Attune NxT Acoustic Focusing Cytometer allows for the identification of multiple T cell subsets and myeloid cells
- 13-parameter immunophenotyping of human lysed whole blood with the the Attune NxT Flow Cytometer allows for the identification of B cells, NK cells, multiple T cell subsets and myeloid cells
- Multiparameter analysis of murine regulatory T cells and dendritic cells with the Attune NxT Acoustic Focusing Cytometer
- Detection of murine regulatory T cells on the Attune NxT Acoustic Focusing Cytometer
No-wash, no lyse applications
- Detection of platelets in whole blood using the Attune NxT Flow Cytometer
- No-wash, no-lyse detection of leukocytes in human whole blood on the Attune NxT Acoustic Focusing Cytometer
- No-wash, no-lyse detection of phagocytic cells via a pHrodo BioParticles functional assay in human whole blood on the Attune NxT Acoustic Focusing Cytometer
Fluorescent protein detection
Stem cells
Literature
Manuals
Posters
Cell types successfully analyzed on the Attune NxT Flow Cytometer
| Sample type | Demonstrated example | Published References |
|---|---|---|
| Algae | Nannochloropsis oculata | |
| Bacteria | Escherichia coli | |
| Bacillus subtilis | ||
| Legionella strains | ||
| Staphyloccus aureus | ||
| Diatom | Phaeodactylum tricornutum | |
| Human | Human adenocarcinoma | |
| Human bone marrow (PBMCs) | ||
| Human bone osteosarcoma cells (U2OS) | ||
| Human cardiomyocytes | ||
| Human corneal epithelial cells | ||
| Human embryonic kidney (293FT) | ||
| Human endothelial cells | ||
| Human leukocytes | ||
| Human mesenchymal stem cell (hMSC) | ||
| Human red blood cells (RBC) | ||
| Human T cell leukemia (Jurkat) | ||
| Human whole blood | ||
| Mouse | Mouse splenocytes | |
| Mouse T and B regulatory cells | ||
| Mouse cardiomyocytes | ||
| Mouse bone marrow | ||
| Mouse lung tissue | ||
| Mouse tumor cells | ||
| Mouse skeletal muscle | ||
| Parasites | Plasmodium falciparum | |
| L. amazonensis, T. brucei and T. cruzi | ||
| Trypanosoma cruzi | ||
| Picophytoplankton | Prochlorococcus spp; Synechococcus spp |
|
| Plants | Arabidopsis thaliana | |
| Phalaris arundinacea | ||
| Solidago canadensis | ||
| Yeast | Candida albicans | |
| Saccharomyces cerevisiae | ||
| Zebra fish | Zebra fish organs |
A flow cytometer designed with your research in mind
- Conserve sample and quickly run experiments with sample flow rates ranging from 12.5–1,000 μL/min
- Intuitive software interface and easy-to-follow workflow that is quick to learn, teach and use
- Save time and minimize cell handling with faster protocols
- Expand your capabilities with up to 6 channels of detection off the violet laser
Investigating flow cytometry instrument capabilities to maximize your research
The 40-page Flow Cytometer Evaluation Guide will enable a better understanding of the components and capabilities of various flow cytometers for objective comparison of instruments from several manufacturers.
Freedom of choice
- Option for up to 6 channels off your violet laser with the Attune NxT Violet Laser Upgrade Kit
- Configurable laser and filters provide more choices when designing multicolor panels
- Broaden your capabilities with field upgradeability of up to 4 lasers and 14 colors
- Expand multiparameter capabilities with detection of dim to very bright signals
- See compatible reagents by instrument configuration or detection channel
An evolutionary flow cytometer
- Acquire sufficient events even from very dilute samples with acoustic-assisted hydrodynamic focusing
- Investigate difficult sample types without fear of clogging
- Protect precious samples and boost success in rare-cell experiments (like this researcher discovered)
Automation for any lab
Improve your workflow for fully automated sample acquisition from 96-well and 384-well microtiter plates. There are no physical changes to the system needed in order to run plate based experiments—everything is controlled in the software.
- Attune Autosampler is compact and compatible with many different types of plates
- No carousel needed; run deep well plates up to 2 mL
- Rapid processing of up to 384 samples
2018 Attune NxT instrument options
This grow-with-you system can be customized with up to 4 lasers and 14 colors and now up to 6 channels off of the violet laser. The optical system was designed to provide optimal signal and has the range to detect very bright as well as dim signals. The Attune NxT Flow Cytometer allows researchers the freedom to build multicolor panels, accommodating common fluorophores and dyes. Build your multicolor panel for the Attune NxT flow cytometer with our superior reagents and antibodies.
Attune NxT Flow Cytometer configuration options
| Lasers | Laser configuration (Cat. No.) |
Lasers included, No. of detection channels | Total detection channels* | ||||
|---|---|---|---|---|---|---|---|
| Violet, 405 nm | Blue, 488 nm | Yellow, 561 nm | Green, 532 nm |
Red, 637 nm | |||
| 1 | Blue (A24864) |
Available as upgrade† |
4
|
Available as upgrade† | Available as upgrade† | Available as upgrade† | 6 |
| 2 | Blue/green (A28995) |
Available as upgrade† |
3
|
4
|
Available as upgrade† | 9 | |
| Blue/yellow (A24861) |
Available as upgrade† |
3
|
4
|
Available as upgrade† | 9 | ||
| Blue/red (A24863) |
Available as upgrade† |
4
|
Available as upgrade† | Available as upgrade† |
3
|
9 | |
| Blue/violet (A24862) |
4
|
4
|
Available as upgrade† | Available as upgrade† | Available as upgrade† | 10 | |
| Blue/violet 6 (A29002) |
6
|
3
|
Available as upgrade† | Available as upgrade† | 11 | ||
| 3 | Blue/green/red (A28997) |
Available as upgrade†
|
3
|
4
|
3
|
12 | |
| Blue/red/yellow (A28993) | Available as upgrade† | 3 | 4 | 3 | 12 | ||
| Blue/green/violet (A28999) |
4
|
3
|
4
|
Available as upgrade† | 13 | ||
| Blue/violet/yellow (A24859) |
4
|
3
|
4
|
|
Available as upgrade† | 13 | |
| Blue/red/violet (A24860) |
4
|
4
|
Available as upgrade† | Available as upgrade† |
3
|
13 | |
| Blue/red/violet 6 (A29003) |
6
|
3
|
Available as upgrade† |
3
|
14 | ||
| 4 | Blue/red/violet/green (A29001) |
4
|
3
|
4
|
3
|
16 | |
| Blue/red/yellow/violet (A24858) |
4
|
3
|
4
|
3
|
16 | ||
| Blue/red/yellow/violet 6 (A29004) |
6
|
2
|
3
|
3
|
16 | ||
| * Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel. † Attune NxT Flow Cytometer owners wishing to purchase a laser upgrade should request a quote here: Yellow Laser Upgrade, Violet Laser Upgrade, Red Laser Upgrade, or Green Laser Upgrade. After we receive your quote request, a technical sales specialist will contact you about upgrade options. |
|||||||
Are you comparing the specifications of more than one flow cytometer?
A specification is derived from a specific test or calculation, but these tests aren’t standardized across instrument developers and may be misleading in a side-by-side comparison because their values may have been calculated in different ways, despite sharing the same terminology. This 8-page Comparing Technical Specification Documents resource offers helpful tips on deciphering variations.
| Optics |
|
||||||||||||||||||||||||||||
| Fluidics |
|
||||||||||||||||||||||||||||
| Performance |
|
||||||||||||||||||||||||||||
| Software |
|
||||||||||||||||||||||||||||
| Quality and regulatory |
|
||||||||||||||||||||||||||||
| Data management |
|
||||||||||||||||||||||||||||
| Installation requirements |
|
||||||||||||||||||||||||||||
Simple yet powerful software, easy auto compensation
Invitrogen Attune NxT Software is designed to provide powerful data acquisition and analysis using an intuitive, user-friendly interface. The instrument software features and benefits include:
- Compensation—fully automated and manual compensation
- Instrument tracking—automated baseline and performance test for all channels and linearity
- Automated maintenance—startup (<15 min), shutdown, rinse, wash, debubble, deep clean,
monthly decontamination - User account maintenance—administrative and individual accounts with user log
- Gates—standard and customizable gates
- Plot previews—easy workspace setup
- Zoom filmstrip viewer—easy visualization and navigation of plots to set gates and plot attributes
- File formats—FCS 3.0 and FCS 3.1
- Graphics resolution—publication-quality images (customizable)
Accessories for the Attune Nxt Flow Cytometer
Find laser upgrade and conversion kits to expand the capabilities of your instrument, as well as filter kits and accessory parts.
Meet the Inventor video series
The Invitrogen Attune NxT Flow Cytometer was developed with acoustic-assisted hydrodynamic focusing technology, and advanced fluidics designed to minimize clogging and effectively handle a broad range of cell types. This allows for a higher degree of data, detail, and throughput that enables processing of a large range of sample types, including large clumpy cells, samples with a low concentration of cells, and precious samples, more quickly and accurately than ever before with no loss in data quality.
The following applications were performed using the Attune NxT Flow Cytometer.
Immunophenotyping
- Validated 10-color T cell panel using the Attune NxT Flow Cytometer
- Attune NxT Acoustic Focusing Cytometer for 6-color immunophenotyping analysis of stained human whole blood using a no-lyse, no-wash protocol, with no compensation
- Ten-parameter immunophenotyping of human lysed whole blood with the Attune NxT Acoustic Focusing Cytometer allows for the identification of multiple T cell subsets and myeloid cells
- 13-parameter immunophenotyping of human lysed whole blood with the the Attune NxT Flow Cytometer allows for the identification of B cells, NK cells, multiple T cell subsets and myeloid cells
- Multiparameter analysis of murine regulatory T cells and dendritic cells with the Attune NxT Acoustic Focusing Cytometer
- Detection of murine regulatory T cells on the Attune NxT Acoustic Focusing Cytometer
No-wash, no lyse applications
- Detection of platelets in whole blood using the Attune NxT Flow Cytometer
- No-wash, no-lyse detection of leukocytes in human whole blood on the Attune NxT Acoustic Focusing Cytometer
- No-wash, no-lyse detection of phagocytic cells via a pHrodo BioParticles functional assay in human whole blood on the Attune NxT Acoustic Focusing Cytometer
Fluorescent protein detection
Stem cells
Literature
Manuals
Posters
Cell types successfully analyzed on the Attune NxT Flow Cytometer
| Sample type | Demonstrated example | Published References |
|---|---|---|
| Algae | Nannochloropsis oculata | |
| Bacteria | Escherichia coli | |
| Bacillus subtilis | ||
| Legionella strains | ||
| Staphyloccus aureus | ||
| Diatom | Phaeodactylum tricornutum | |
| Human | Human adenocarcinoma | |
| Human bone marrow (PBMCs) | ||
| Human bone osteosarcoma cells (U2OS) | ||
| Human cardiomyocytes | ||
| Human corneal epithelial cells | ||
| Human embryonic kidney (293FT) | ||
| Human endothelial cells | ||
| Human leukocytes | ||
| Human mesenchymal stem cell (hMSC) | ||
| Human red blood cells (RBC) | ||
| Human T cell leukemia (Jurkat) | ||
| Human whole blood | ||
| Mouse | Mouse splenocytes | |
| Mouse T and B regulatory cells | ||
| Mouse cardiomyocytes | ||
| Mouse bone marrow | ||
| Mouse lung tissue | ||
| Mouse tumor cells | ||
| Mouse skeletal muscle | ||
| Parasites | Plasmodium falciparum | |
| L. amazonensis, T. brucei and T. cruzi | ||
| Trypanosoma cruzi | ||
| Picophytoplankton | Prochlorococcus spp; Synechococcus spp |
|
| Plants | Arabidopsis thaliana | |
| Phalaris arundinacea | ||
| Solidago canadensis | ||
| Yeast | Candida albicans | |
| Saccharomyces cerevisiae | ||
| Zebra fish | Zebra fish organs |
3D Demo: Explore the Attune NxT Flow Cytometer and Autosampler
A software tool for exploring the hardware, software, optics and fluidics of the Attune NxT Flow Cytometer. Hot spots throughout the tool feature data, researcher opinions, and technical specifications. Mouse operation enables rotation, zoom, and measurement. Available in 8 languages.
Service plans
Optimize your instrument performance, maximize the availability of your critical systems, and streamline your laboratory workflows. Choose the level of support for your Attune NxT Flow Cytometer that best meets your laboratory's needs and budget:
- AB Complete Plan
- AB Assurance Plan
- AB Maintenance PLUS Plan
Consumables and accessories
In addition to accessories such as sample syringes, find the Invitrogen Attune solutions necessary to operate your Attune NxT Flow Cytometer including:
- Attune Focusing Fluid
- Attune Wash Solution
- Attune Shutdown Solution
- Attune Performance Tracking Beads
Flow Cytometry Panel Builderbeta
This online tool guides you through flow cytometry panel design, providing a simplified, customizable experience to fit your flow cytometry panel design needs.
Fluorophore Selection Guide
Use these quick guides to find antibodies and other conjugates of a broad range of fluorescent dyes, fluorescent proteins, Qdot probes, and phycobiliproteins.
Fluorescence SpectraViewer
This online tool allows researchers to assess the spectral compatibility of dyes and probes in the course of designing experiments that utilize fluorescence detection techniques.
Resources
Learning center
Flow Cytometry Learning Center
Access flow cytometry application resources for more success as you plan and execute your experiments.
Support
Flow Cytometry Support Center
Find tips, troubleshooting help, and resources for your flow cytometry workflow.