Attune NxT Flow Cytometer | Thermo Fisher Scientific

Clogging has met its match

Minimize the hassle, frustration, and sample-loss associated with a clogged instrument
Investigate difficult samples and large, clumpy cells, including cancer cells, cardiomyocytes, and adipocytes, which were previously not amenable to flow cytometry

Efficient. Flexible. Transformative.

The 2018 Attune NxT Flow Cytometer system makes multiparametric flow cytometry available to both new and experienced researchers. Experience the difference acoustic assisted hydrodynamic focusing technology brings to your workflow. Designed for faster experimental run times, this compact flow cytometer offers proven, rapid and accurate analysis for a broad range of sample types.

Efficient—up to 10X faster than traditional flow cytometric analyzers
Flexible—up to 14 colors; up to 6 channels off the violet laser
Transformative—run difficult samples with a system that is less sensitive to clogging

A flow cytometer designed with your research in mind

Conserve sample and quickly run experiments with sample flow rates ranging from 12.5–1,000 μL/min
Intuitive software interface and easy-to-follow workflow that is quick to learn, teach and use
Save time and minimize cell handling with faster protocols
Expand your capabilities with up to 6 channels of detection off the violet laser

Investigating flow cytometry instrument capabilities to maximize your research

The 40-page Flow Cytometer Evaluation Guide will enable a better understanding of the components and capabilities of various flow cytometers for objective comparison of instruments from several manufacturers.

Request the guide

Freedom of choice

Option for up to 6 channels off your violet laser with the Attune NxT Violet Laser Upgrade Kit
Configurable laser and filters provide more choices when designing multicolor panels
Broaden your capabilities with field upgradeability of up to 4 lasers and 14 colors
Expand multiparameter capabilities with detection of dim to very bright signals
See compatible reagents by instrument configuration or detection channel

An evolutionary flow cytometer

Acquire sufficient events even from very dilute samples with acoustic-assisted hydrodynamic focusing
Investigate difficult sample types without fear of clogging
Protect precious samples and boost success in rare-cell experiments (like this researcher discovered)

Automation for any lab

Improve your workflow for fully automated sample acquisition from 96-well and 384-well microtiter plates. There are no physical changes to the system needed in order to run plate based experiments—everything is controlled in the software.

Attune Autosampler is compact and compatible with many different types of plates
No carousel needed; run deep well plates up to 2 mL
Rapid processing of up to 384 samples

Learn more

2018 Attune NxT instrument options

This grow-with-you system can be customized with up to 4 lasers and 14 colors and now up to 6 channels off of the violet laser. The optical system was designed to provide optimal signal and has the range to detect very bright as well as dim signals. The Attune NxT Flow Cytometer allows researchers the freedom to build multicolor panels, accommodating common fluorophores and dyes. Build your multicolor panel for the Attune NxT flow cytometer with our superior reagents and antibodies.

Attune NxT Flow Cytometer configuration options

Lasers	Laser configuration (Cat. No.)	Lasers included, No. of detection channels					Total detection channels*
Lasers	Laser configuration (Cat. No.)	Violet, 405 nm	Blue, 488 nm	Yellow, 561 nm	Green, 532 nm	Red, 637 nm	Total detection channels*
1	Blue (A24864)	Available as upgrade†	4	Available as upgrade†	Available as upgrade†	Available as upgrade†	6
2	Blue/green (A28995)	Available as upgrade†	3		4	Available as upgrade†	9
	Blue/yellow (A24861)	Available as upgrade†	3	4		Available as upgrade†	9
	Blue/red (A24863)	Available as upgrade†	4	Available as upgrade†	Available as upgrade†	3	9
	Blue/violet (A24862)	4	4	Available as upgrade†	Available as upgrade†	Available as upgrade†	10
	Blue/violet 6 (A29002)	6	3	Available as upgrade†		Available as upgrade†	11
3	Blue/green/red (A28997)	Available as upgrade†	3		4	3	12
	Blue/red/yellow (A28993)	Available as upgrade†	3	4		3	12
	Blue/green/violet (A28999)	4	3		4	Available as upgrade†	13
	Blue/violet/yellow (A24859)	4	3	4		Available as upgrade†	13
	Blue/red/violet (A24860)	4	4	Available as upgrade†	Available as upgrade†	3	13
	Blue/red/violet 6 (A29003)	6	3	Available as upgrade†		3	14
4	Blue/red/violet/green (A29001)	4	3		4	3	16
	Blue/red/yellow/violet (A24858)	4	3	4		3	16
	Blue/red/yellow/violet 6 (A29004)	6	2	3		3	16
* Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel. † Attune NxT Flow Cytometer owners wishing to purchase a laser upgrade should request a quote here: Yellow Laser Upgrade, Violet Laser Upgrade, Red Laser Upgrade, or Green Laser Upgrade. After we receive your quote request, a technical sales specialist will contact you about upgrade options.

Reagents Antibodies Panel Builder

Are you comparing the specifications of more than one flow cytometer?

A specification is derived from a specific test or calculation, but these tests aren’t standardized across instrument developers and may be misleading in a side-by-side comparison because their values may have been calculated in different ways, despite sharing the same terminology. This 8-page Comparing Technical Specification Documents resource offers helpful tips on deciphering variations.

Download the tip sheet

Comparing technical specification document

Optics

Laser power (as shown in table below)

Laser	Wavelength (nm)	*Beam-shaping optics (BSO) (mW)**	Diode power (mW)**
Violet	405	50	100
Blue	488	50	100
Green	532	100	140
Yellow	561	50	100
Red	637	100	140
* Amount of measured usable laser power after light has gone through the beam optics and shaping filters. ** Vendor-specified theoretical maximum.

Laser excitation: Optimized excitation for minimized stray laser-line noise and losses to reflection
Laser profile: 10 μm x 50 μm flat-top laser providing robust alignment
Emission filters: Up to 14 color channels with wavelength-tuned photomultiplier tubes (PMTs); user-changeable, keyed filters
Laser separation: 150 μm
Optical alignment: Fixed alignment with prealigned welded fiber; no user maintenance require
Onboard thermal-electric cooler: No warm-up delay; fiber isn't affected by on/off
Simmer mode: Instant on/off reduces usage and/or aging by 10x; only keep it "on" when acquiring samples; reports hours of usage
Flat top specified at the flow cell: CV <3% over width of flat top
Upgradable: Convenient field changes

Fluidics

Flow cell: Quartz cuvette gel coupled to 1.2 numerical aperture (NA) collection lens, 200 x 200 μm
Sample analysis volume: 20 μL to 4 mL
Custom sample flow rates: 12.5–1,000 μL/min
Sample delivery: Positive-displacement syringe pump for volumetric analysis
Sample tubes: Accommodates tubes from 17 x 100 mm to 8.5 x 45 mm
Fluid-level sensing: Active
Standard fluid reservoirs: 1.8 L focusing fluid tank, 1.8 L waste tank, 175 mL shutdown solution tank, and 175 mL wash solution tank
Fluid storage: All fluids stored within instrument
Extended fluidics option: Configuration for 10 L fluid
Nominal fluid consumption: 1.8 L/day
Automated maintenance cycles: ≤15 min startup and shutdown—deep clean, sanitize, and debubble modes

Performance

Fluorescence sensitivity: ≤80 MESF for FITC, ≤30 MESF for PE, ≤70 MESF for APC
Fluorescence resolution: CV <3% for the singlet peak of propidium iodide–stained chicken erythrocyte nuclei (CEN)
Data acquisition rate: Up to 35,000 events/sec, 34 parameters, based on a 10% coincidence rate per Poisson statistics
Maximum electronic speed: 65,000 events/sec with all parameters
Carryover: Single-tube format: <1%
Forward and side scatter sensitivity: Able to discriminate platelets from noise
Forward and side scatter resolution: Optimized to resolve lymphocytes, monocytes, and granulocytes in lysed whole blood
Forward scatter: Photodiode detector with 488/10 nm bandpass filter
Side scatter: PMT with default 488/10 nm bandpass filter; optional 405/10 nm bandpass filter
Fluorescent detectors: 14 individual detectors
Electronic pulse: Measured area, height and width pulse for all detectors
Violet side scatter resolution: Can be configured for violet side scatter to better resolve particles from noise
Minimum particle size: 0.2 μm on side scatter using Bangs Laboratories, Inc.™ Submicron Bead Calibration Kit (Cat. No. 832)

Software

Compensation: Full matrix—automated and manual modes, on-plot compensation tools for fine adjustment; use of tubes and wells
Flow rate: Precise flow rate control via software; no hardware adjustments
Live streaming: Live update of statistics during acquisition of events up to 35,000 events/sec
Overlays: Comparative analysis between samples; 3D view
Sample recovery: System able to return unused samples
Concentration: Direct concentration measurement without use of counting beads
Software layout: Fully customizable for each user account
Bubble detection technology: Stops automated run to preserve sample integrity
Maximum single-event file: 20 million with option to append
Heat map: Set up for definition of plate layout; screening view for analysis for tubes and plates
Threshold: Up to 4 individual thresholds with user option to apply Boolean logic
Gating: Hierarchal gating with the ability to derive gates
Smartgate labeling: Option to annotate quad gate names based on fluorophore and target names
Voltage: User adjustable
Window extensions: User adjustable
Area scaling factor (ASF): User adjustable
Acquisition settings: Documented in FCS files and maintained upon import
Templates: Create from existing experiments—instrument settings, workspaces, run protocols, heat map settings, and compensation settings optimized and defined previously
Tube-to-plate conversion: One-click transition from tubes to plates and vice versa; no disassembly, no additional QC, no reboot required for conversion between plates and tubes
Graphics resolution: Publication-quality images; support for TIF, PNG, BMP, JPG, GIF, and EMF; quickly copy and paste plots to any external application (e.g., Microsoft™ PowerPoint™ software)
User account administration: Administrative creation of individual user accounts with designated roles, advanced setting permissions, management of individual accounts, user time tracking, and sample count

Quality and regulatory

Instrument tracking: Automated daily baseline and performance test with Levey-Jennings plots
Warranty: 1 year
Production verification testing: Each instrument is tested and verified for assembly integrity and performance to specifications
Quality management system: Manufacturing standards comply with the requirements of ISO 13485:2003
Robust install specifications: Units installed by engineer; preplanning checklist, delivery, installation, and performance validation comply with standardized procedure
For Research Use Only

Data management

Software requirements: Invitrogen Attune NxT Software
Monitor: 23-inch flat panel (1,920 x 1,080 resolution); dual-monitor capability
Computer: Minitower desktop
Operating system: Windows™ 7 64-bit
FCS format: FCS 3.1, 3.0
Processor: Intel Core™ i7 processor
RAM: 32 GB
Hard drives: 2 x 2TB SATA 6.0Gb/s, 7200 RPM, 64MB data burst cache; Controller RAID1, integrated

Installation requirements

Electrical requirements: 100–240 VAC, 50/60 Hz, <150 W. Thermo Fisher Scientific certifies that the Attune NxT Flow Cytometer conforms to relevant directives to bear the CE mark. The instrument also conforms to the UL and CAN/CSA general requirements (61010.1). The Attune NxT Flow Cytometer is a Class I laser product per CDRH regulations and EN/IEC 60825.
Heat dissipation: <150 W
Temperature operating ranges: 15–30°C (59–86°F)
Operating humidity: 10–90%, noncondensing
Audible noise: <65 dBA at 1.0 m
Instrument size (H x W x D): ~40 x 58 x 43 cm (16 x 23 x 17 in.), including fluid bottles
Weight: ~29 kg (64 lb)
Available configurations (see Attune NxT Flow Cytometer configuration options table)

Download the Attune NxT Flow Cytometer Specification Sheet

Simple yet powerful software, easy auto compensation

Invitrogen Attune NxT Software is designed to provide powerful data acquisition and analysis using an intuitive, user-friendly interface. The instrument software features and benefits include:

Compensation—fully automated and manual compensation
Instrument tracking—automated baseline and performance test for all channels and linearity
Automated maintenance—startup (<15 min), shutdown, rinse, wash, debubble, deep clean,
monthly decontamination
User account maintenance—administrative and individual accounts with user log
Gates—standard and customizable gates
Plot previews—easy workspace setup
Zoom filmstrip viewer—easy visualization and navigation of plots to set gates and plot attributes
File formats—FCS 3.0 and FCS 3.1
Graphics resolution—publication-quality images (customizable)

Learn more about software for the Attune NxT Flow Cytometer

Accessories for the Attune NxT Flow Cytometer

Find laser upgrade and conversion kits to expand the capabilities of your instrument, as well as filter kits and accessory parts.

Attune NxT upgrades	Filter	Common fluorochromes	Cat. No.
Attune NxT Yellow Laser Upgrade Kit	585/16	PE, Alexa Fluor 532, Alexa Fluor 568	100022779
	620/15	Alexa Fluor 594, PE-Texas Red, PE-Alexa Fluor 610
	695/40	PE-Alexa Fluor 700, TRI-COLOR (PE-Cy5), PE-Cy5.5, Qdot 705
	780/60	Qdot 800, PE-Cy 7, PE-Alexa Fluor 750
Attune NxT Red Laser Upgrade Kit	670/14	APC, Alexa Fluor 647, Qdot 655	100022778
	720/30	Alexa Fluor 680, Alexa Fluor 700, APC-Alexa Fluor 700, Qdot 705
	780/60	APC-Alexa Fluor 750, APC-Cy7, APC-eFluor 780, Qdot 800
Attune NxT Green Laser Upgrade Kit	575/36	PE, Alexa Fluor 532, Alexa Fluor 555, DsRed	A32701
	620/15	Alexa Fluor 568, Alexa Fluor 594, Qdot 605, PE-Texas Red, PE-Alexa Fluor 610
	695/40	Qdot 705, PE-Alexa Fluor 700, TRI-COLOR, PE-Cy5.5
	780/60	Qdot 800, PE-Cy7
Attune NxT Violet Laser Upgrade Kit	440/50	Super Bright 436, Alexa Fluor 405, eFluor 450, Pacific Blue	100022777
	512/25	eFluor 506, Pacific Green
	603/48	Super Bight 600, Pacific Orange, Qdot 605
	710/50	Super Bright 702, Qdot 705
Attune NxT Violet 6 Conversion Kit, Blue Laser	530/30	Alexa Fluor 488, FITC	A35428
	574/26	PE, PE-Alexa Fluor 610, Alexa Fluor 546
	695/40	PE-Alexa Fluor 700, PE-Cy5.5, PerCP, PerCP-Cy5.5, Qdot 705, TRI-COLOR
Attune NxT Violet 6 Conversion Kit, Violet Laser	450/40	Super Bright 436, eFluor 450, Pacific Blue	A36569
	525/50	eFluor 506, Pacific Green, eFluor 506
	610/20	Super Bright 600, Qdot 605, Pacific Orange
	660/20	Super Bright 645
	710/50	Super Bright 702, Qdot 705
	780/60	Super Bright 780
Attune NxT Violet 6 Conversion Kit, Red Laser	670/14	APC, Alexa Fluor 647, Qdot 655	A36571
	720/30	Alexa Fluor 680, Alexa Fluor 700, APC-Alexa Fluor 700, Qdot 705
	780/60	APC-Alexa Fluor 750, APC-eFluor 780, APC-Cy7, Qdot 800
Attune NxT Violet 6 Conversion Kit, Yellow Laser	585/16	PE, Alexa Fluor 532, Alexa Fluor 568	A36572
	620/15	Alexa Fluor 594, PE-Texas Red, PE-Alexa Fluor 610
	695/40	PE-Alexa Fluor 700, TRI-COLOR, PE-Cy5.5, Qdot 705
	780/60	Qdot 800, PE-Cy 7, PE-Alexa Fluor 750

The Invitrogen Attune NxT Flow Cytometer was developed with acoustic-assisted hydrodynamic focusing technology, and advanced fluidics designed to minimize clogging and effectively handle a broad range of cell types. This allows for a higher degree of data, detail, and throughput that enables processing of a large range of sample types, including large clumpy cells, samples with a low concentration of cells, and precious samples, more quickly and accurately than ever before with no loss in data quality.

The following applications were performed using the Attune NxT Flow Cytometer.

Immunophenotyping

No-wash, no lyse applications

Literature

Manuals

Posters

Cell types successfully analyzed on the Attune NxT Flow Cytometer

Sample type	Demonstrated example	Published References
Algae	Nannochloropsis oculata
Bacteria	Escherichia coli	Nucleic Acids Res 44(17):e139 (2016) Metab Eng 38:370 (2016) Methods 16 Feb (2018) ACS Synth Biol 7(1):166 (2018) J Am Chem Soc 138(22):7040 (2016) Cell Chem Biol 23(12):1539 (2016)
	Bacillus subtilis	Nucleic Acids Res 44(15):7495 (2016)
	Legionella strains	Water Research 11 April (2018)
	Staphyloccus aureus
Diatom	Phaeodactylum tricornutum	Sci Total Environ 634:87 (2018)
Human	Human adenocarcinoma	Oncotarget 7(17):23772 (2016)
	Human bone marrow (PBMCs)	J Cell Mol Med 22(5):2706 (2018) Immunol Lett 182:30 (2017) Cancer Immunol Immunother 67(3):381 (2018)
	Human bone osteosarcoma cells (U2OS)	Mol Med Rep 12(4): 5042 (2015)
	Human cardiomyocytes
	Human corneal epithelial cells
	Human embryonic kidney (293FT)	J Biotechnol 241:136 (2017) Biomater Sci Eng 2(11): 2080 (2016) Sci Rep 6:29139 (2016) Curr Protoc Cytom 82:7.49.1 (2017) Acta Biomater 69:265 (2018) Biotechnol Lett 38(6):919 (2016)
	Human endothelial cells	Bioeng Transl Med 1(1):103 (2016) Stem Cells Transl Med 7(1):59 (2018)
	Human leukocytes
	Human mesenchymal stem cell (hMSC)	Stem Cell Res Ther 8(1):31 (2017) Exp Cell Res. 337(2):160 (2015)
	Human red blood cells (RBC)
	Human T cell leukemia (Jurkat)	Nat Commun 9:253 (2018) Sci Rep 8:3846 (2018)
	Human whole blood	Methods 134-135:149 (2018)
Mouse	Mouse splenocytes	PloS One 13(4): e0193236 (2018)
	Mouse T and B regulatory cells	Acupunct Med Mar 26 (2018)
	Mouse cardiomyocytes	Braz J Med Biol Res 50(2):e5760 (2017)
	Mouse bone marrow	Biomaterials 128:19 (2017)
	Mouse lung tissue	Acupunc Med 26 March (2018) doi:
	Mouse tumor cells	Sci Rep 7:42687 (2017)
	Mouse skeletal muscle	Muscle Nerve 57(6):1006 (2018)
Parasites	Plasmodium falciparum	Int J Parasitol Drugs Drug Resist 7(1):120 (2017)
	L. amazonensis, T. brucei and T. cruzi	J Eukaryot Microbiol 64(6):756 (2017)
	Trypanosoma cruzi	Eur J Med Chem 139:947 (2017)
Picophytoplankton	Prochlorococcus spp; Synechococcus spp
Plants	Arabidopsis thaliana
	Phalaris arundinacea	Ann Bot 121(7):1309 (2018)
	Solidago canadensis	Oecologia 182(3):755 (2016)
Yeast	Candida albicans	Sci Rep 7:43542 (2017) Med Mycol 56(5):565 (2017)
Yeast	Saccharomyces cerevisiae	PNAS 201715996: 12 Mar (2018) Commun Biol 1:7 (2018)
Zebra fish	Zebra fish organs	J Immunol 197(9):3520 (2016)

Service plans
Planned maintenance
Compliance services

Technical & Application Support
Education services
Instrument management

On-site service plans at a glance

Increase your lab productivity and uptime, enabling you to focus on what matters most, your research success.

Product	AB Complete	AB Assurance	AB Maintenance
Standard on-site response time	Next business day*	2 business days*	3 business days**
Scheduled on-site PM
Remote instrument diagnostics
Priority access to technical support (Monday–Friday, standard business hours)
Parts, labor, and travel for repair
Priority access to remote service engineer
Post-PM requalification and critical repairs
Field applications scientist (FAS) consultation

* Availability limited in some geographic areas.
** After receipt of a purchase order.

Request a quote

Planned maintenance

Planned Maintenance service for the Attune NxT Flow Cytometer includes:

Performance assurance checks
Functionality and safety checks
Safety inspections in accordance with all applicable regulations
Interior and exterior cleaning of the instrument
Components check up
Software update
Routine inspection and replacement of the following:
Updates relating to all service bulletins relevant to instrument configuration

Checks completed during planned maintenance visit:
System maintenance	visually inspect all cables clean the interior and the exterior of unit clean the cove tray and visor clean the tube lifter check the tube lifter for proper operation
Computer maintenance	run the disk cleanup defragment the disk, only if the operating system indicates that it is recommended
Fluidics system maintenance	replace the SIP tube inspect the instrument internally for any traces of salt residue check for internal leaks and damage at all fittings, valves, pumps and syringe pump replace the 1 mL syringe run the system decontamination script, if not previously done by the customer replace both 0.2 micron filters, if not previously done by the customer
Optics maintenance	inspect all optical filters. Clean only if necessary verify the laser alignment, including the pinholes and blocker bar
System diagnostics	run the System Test and ensure that everything passes ensure that the latest Attune NxT Software version is installed check leak detector functionality record laser hours
Attune NxT Autosampler maintenance (if applicable to unit)	run the Attune NxT Autosampler calibration script replace syringe
Instrument performance	ensure that the baseline and performance tests pass the specifications

Compliance services

Get timely, cost-effective, and audit-ready documentation managed by a compliance specialist to help ensure your Attune NxT Flow Cytometer system is installed, operating, and performing to the manufacturer’s specifications.

Verify instrument performance and minimize downtime with IQ/OQ/IPV Services

Our factory-trained and experienced engineers will conduct Installation Qualification (IQ), Operational Qualification (OQ), and Instrument Performance Verification (IPV) on the Invitrogen Attune NxT Flow Cytometer system in your lab. Documentation for audits is provided.

Find compliance documents for Installation and Operational Qualification (IQ/OQ), and Instrument Verification (IPV) for your systems

Technical & Application Support

Take the hassle out of your hands with custom solutions

Assay development & labeling services—our custom solutions to help develop the most appropriate assay for your research or discovery target of interest.

Custom antibodies—our custom antibody development, purification, and screening services deliver the quality, consistency, and performance you need.

Education services

Application and instrument training programs are available at our training centers located throughout the world, within your lab, or through web-based instruction. We offer a wide range of courses to build the skills of cellular analysis professionals and users of the Attune NxT Flow Cytometer:

Training Center Courses—get away from the day-to-day responsibilities of the lab and dedicate time to focus on your education needs. Courses are hosted at Thermo Fisher Scientific Training Center sites to provide professional, interactive education that includes classroom instruction and hands-on training in the laboratory.
Customer Site/content—courses are offered at your location by a Field Application Scientist (FAS) to provide professional, interactive education that includes hands-on instruction in your laboratory.
LabCoat Live—learn a specific application in as little as 1 week. Courses provide interactive education via online lectures and a self-paced hands-on experiment using provided protocols and reagents that we ship to your lab.
eLearning—discover new areas of interest and learn about life science topics at your own pace; courses provide education via on-demand, pre-recorded webinars.

Learn more about our Flow Cytometry Webinars
Take our T Cell Stimulation and Proliferation eLearning Course

Instrument management

Manage your instrument use and care online with Instrument Management. Whether you are responsible for one instrument or hundreds of instruments, Instrument Management enables you to easily manage your instrument use and care online.

24/7 access to complete instrument service history and field service reports
Request online service and service contract quotes
Instrument software, patches, and updates
Online instrument scheduler for instrument availability

Online instrument scheduler for instrument availability

Find out more about Instrument Management at thermofisher.com/easiertomanage

Benefits

A flow cytometer designed with your research in mind

Conserve sample and quickly run experiments with sample flow rates ranging from 12.5–1,000 μL/min
Intuitive software interface and easy-to-follow workflow that is quick to learn, teach and use
Save time and minimize cell handling with faster protocols
Expand your capabilities with up to 6 channels of detection off the violet laser