Complex Gene Synthesis | Thermo Fisher Scientific

Researchers who need long custom DNA constructs for pharmaceutical development, such as viral genomes, or for metabolic engineering, pathway engineering, or synthetic biology can avail our proprietary Invitrogen GeneArt Gene Synthesis services. Our scientists have experience generating longer constructs from 10 kb to several hundred kilobases or containing complex sequence features.

GeneArt services for complex gene synthesis

Using our advanced GeneArt technology, even relatively complex sequences can be synthesized and assembled rapidly and reliably. Complex genes and sequences may have challenging features such as complex secondary structures, repetitive sequences (direct or indirect), high (>80%) or low (<20%) GC content (Figure 1), or long polypurine/polypyrimidine runs that cannot be synthesized using our standard process. For these projects, we have developed a unique proprietary technology that employs specially modified and purified oligonucleotides, and more sophisticated gene assembly methods.

In many cases these projects can be completed and shipped with the same turnaround time as our conventional gene synthesis projects.

Plot showing the GC content in a 40 bp window centered at each nucleotide position along a DNA sequence. The 5' end, the exhibits very high GC content.

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Figure 1. Example of sequence with very high GC content. The plot shows the GC content in a 40 bp window centered at the indicated nucleotide position. In this example, ~300 bp of the 5’ end of the sequence has a very high GC content.

For details on our Complex Gene Synthesis service, including a price and delivery estimate, upload your sequence for analysis by going to our secure online GeneArt Services Dashboard.

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Advantages of custom DNA synthesis services for long and complex DNA constructs

Our high-throughput gene synthesis capacity of >15 Mbp per month allows fast and reliable production of DNA pieces for assembly. Our other capabilities include:

Expert design and assembly technologies and process expertise for the assembly of constructs from 10 kb to several hundred kilobases, with timelines starting from 23 business days
Robust technologies to build DNA constructs with high complexity
Sanger sequencing and next-generation sequencing offer flexible solutions for sequence verification and final QC
Track record of reliable and timely production for long and complex constructs
Proprietary technologies to build high-quality gene fragments up to 3 kb, and assembly kits that enable do-it-yourself construction and assembly

We serve over 3,000 customers on over 12,000 gene constructs each month, so you can be sure we have the global capacity to meet your research needs.

Faster delivery of large and complex genes

Cloned genes ranging in sizes up to 1,000 bp can be completed in as fast as 5 business days.

Table 1. Turnaround times for complex gene synthesis projects.

DNA type	Genes/ORFs	Devices Genes/ORFs	Genetic circuits Enzymatic pathways Recombinant viral genomes	Bacterial genomes Eukaryotic chromosomes (e.g., yeast)
Length	0.1–1 kb	1–7 kb	7 kb–100 kb	100 kb–1.5 Mb
Production time	5–10 business days	12–22 business days	From 23 business days	On request
Service workflow & capabilities	High-throughput production High speed, >15 Mbp/month Leading Bio IT solutions, including GeneOptimizer technology Easy online ordering through GeneArt Dashboard		Specialized manufacturing team Experienced design and assembly Proprietary technology Next-generation sequencing Track record for reliable, timely production of complex custom constructs	Combined expert services and tools Assembly services and tools High-quality DNA fragments Large gene synthesis blocks Large construct assembly Versatile assembly technology and kit solutions
How to order	Order online		Contact us