The High Capacity cDNA Reverse Transcription Kit (formerly the High Capacity cDNA Archive Kit) delivers extremely high quality first-strand cDNA from total RNA. The kit was designed for synthesizing cDNA for short or long term archiving; it yields very quantitative reverse transcription products from 0.02 to 2 μg of input total RNA in a 20 μL reaction (up to 10 μg RNA for a 100 μL reaction). Downstream applications include real-time PCR, standard PCR, and labeling for microarray analysis.

Benefits of this kit include:

  • Linear amplification for most targets in real-time PCR
  • Higher yields and precision at a fraction of the cost

For a master mix in a convenient one-tube format, check out the SuperScript™ IV VILO™ Master Mix. The pairing of SuperScript™ IV reverse transcriptase with an enhanced buffer formulation offers reliable first-strand cDNA synthesis.

Optimum performance in real-time PCR

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription (RT) kit designed for optimum performance in real-time PCR with TaqMan® Gene Expression Master Mix, Power SYBR™ Green PCR Master Mix, and others. The components of the two-tube kit work together to provide sensitive and specific reverse transcription across a broad range of template quantities. The High Capacity RNA-to-cDNA Kit formulation reduces RT time by at least half, requires few pipetting steps, and delivering high cDNA yields.

The High Capacity RNA-to-cDNA Kit consolidates the components of the RT reaction into two tubes; 2X RT buffer and 20X enzyme mix, including RNase inhibitor. These components have been optimized to provide superior cDNA archiving for real-time gene expression analysis, and a more streamlined workflow is achieved by minimizing the the number of reagent additions.