High-Capacity cDNA Reverse Transcription Kit product photo

True to its name, the High-Capacity cDNA synthesis kits produce high quality first-strand cDNA from total RNA. The High-Capacity cDNA Reverse Transcription Kit (formerly the High-Capacity cDNA Archive Kit) is designed for synthesizing cDNA for short- or long-term archiving. The kits yield quantitative reverse transcription products from RNA inputs ranging between 0.02 and 2 μg in a 20 μL reaction; it can be scaled up to 10 μg of input RNA in a 100 μL reaction. Downstream applications include real-time PCR, standard PCR, and labeling for microarray analysis.

High-capacity cDNA synthesis kits: formats

Benefits of the High-Capacity RNA-to-cDNA Kit

The High-Capacity RNA-to-cDNA kit consolidates the reagents for reverse transcription reaction into two tubes: 2X RT buffer mix and 20X enzyme mix, which contains reverse transcriptase and RNase inhibitor. These components have been optimized to provide high cDNA yields for real-time gene expression analysis. The two-tube format streamlines the workflow by minimizing the number of pipetting steps and is designed for optimum performance in real-time PCR with TaqMan Fast Advanced Master Mix and PowerUp SYBR Green Master Mix.

Comparison of cDNA synthesis kits for qPCR

For exceptional performance, explore SuperScript IV Reverse Transcriptase—available as a standalone enzyme, first-strand cDNA synthesis kit, master mix for qRT-PCR applications, and one-step RT-PCR system to suit all your research needs.

Use the selection tool to find the right product for of your cDNA synthesis experiments

 High-Capacity cDNA Reverse Transcription kit
High-Capacity RNA-to-cDNA kitSuperScript IV VILO Master Mix
SuperScript IV VILO Master Mix with ezDNase Enzyme

Value choice for routine applications

cDNA synthesis components in two-tube format

Exceptional master mix for two-step RT-qPCR

Exceptional master mix for two-step RT-qPCR with fast and easy gDNA removal

Reverse transcriptase



SuperScript IV

SuperScript IV

Optimal reaction temperature37°C37°C50°C50°C
Reaction time120 min30 to 60 min10 min10 min
Ability to work with degraded or inhibitor-containing RNALowLowHighHigh
Reduced RNase H activityNoNoYesYes
Primers includedRandom primersRandom primersRandom primers and oligo(dT)20Random primers and oligo(dT)20
Integrated gDNA removalNoNoNoYes*
FormatRT reaction components in separate tubes
RT reaction components in two-tube formatAll RT reaction components premixedAll RT reaction components premixed

*Formats supplied with ezDNase enzyme



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Tm calculator
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For Research Use Only. Not for use in diagnostic procedures.