Generate complex reconstructed 3D volumes with speed and ease. Inspect 3D  is the complete solution for dual- and single axis electron tomography (ET) using Thermo Scientific Krios G4 Cryo-TEM or Glacios Cryo-TEM transmission electron microscopes.

Inspect 3D post alignment and reconstruction software provides a fast, easy, complete solution for dual- and single axis electron tomography.

Software for Electron Microscopes

3D Tomography with Inspect 3D Software

Electron tomography software for volume reconstruction.


Key Features

  • Cross-correlation requires no markers as the process is enhanced by the use of a large variety of filters and image processing tools.
  • Feature tracking can utilize added artificial features like gold markers, or recognizable intrinsic specimen features, as a basis for image alignment.
  • Bead cloaking removes gold beads from the image data after they have been used for alignment, so they do not show in the reconstruction. 
  • The algebraic reconstruction technique iteratively compares projections of the reconstruction to the original projections and corrects the reconstruction to minimize differences.
  • The simultaneous iterative reconstruction technique preserves full spatial information and can enhance the signal to noise ratio of the reconstruction. 
 
Tomography post-alignment and reconstruction module
Tomography post-alignment and reconstruction module
Inspect 3D reconstructed 3D volume screen capture
Inspect 3D reconstructed 3D volume screen capture

Applications

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Infectious Disease Research

Cryo-EM techniques enable multiscale observations of 3D biological structures in their near-native states, informing faster, more efficient development of therapeutics.

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Plant Biology Research

Fundamental plant biology research is enabled by cryo electron microscopy, which provides information on proteins (with single particle analysis), to their cellular context (with tomography), all the way up to the overall structure of the plant (large volume analysis).

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Pathology Research

Transmission electron microscopy (TEM) is used when the nature of the disease cannot be established via alternative methods. With nano-biological imaging, TEM provides accurate and reliable insight for certain pathologies.

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Single Particle Analysis

Single particle analysis (SPA) is a cryo electron microscopy technique that enables structural characterization at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies.

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Cryo-Tomography

Cryo electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

Learn more ›

Single Particle Analysis

Single particle analysis (SPA) is a cryo electron microscopy technique that enables structural characterization at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies.

Learn more ›

Cryo-Tomography

Cryo electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

Learn more ›


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Electron microscopy services for
the life sciences

To ensure optimal system performance, we provide you access to a world-class network of field service experts, technical support, and certified spare parts.

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