Vanquish Duo UHPLC Systems for Inverse Gradient

Uniform analyte response with Charged Aerosol Detection.

Vanquish Duo HPLC and UHPLC Systems for Inverse Gradient

Improve your lab's quantification capabilities with the Thermo Scientific Vanquish Duo HPLC and UHPLC Systems for Inverse Gradient, employing the unique and universal Charged Aerosol Detection, to consistently quantify all non-volatile and semi-volatile compounds even when no reference standard is available. 


Highlights

Universal detection

The Vanquish Duo for Inverse Gradient uses the unique and versatile Charged Aerosol Detector (CAD), a universal detector for the sensitive measurement of non- and semi-volatile compounds – from small molecule to biomolecules, foods and beverages, specialty chemicals or polymers. 

Uniform response for gradient elution

Changing organic composition over a gradient elution results in fluctuation in analyte response. Post column application of an inverse gradient compensation eliminates this effect by ensuring the eluent entering the detector always has the same solvent composition regardless of where the compound elutes during the gradient separation. 

Standard-free quantification

Consistent detector response means that unknown substances can be quantified, even if you lack an individual standard of a specific compound.

Simplified method setup

Effortlessly apply an inverse gradient with Thermo Scientific Chromeleon Chromatography Data System (CDS) wizards that take all system volumes into account and automatically calculate all required parameters for you.

Easy and reliable tool-free connections

The fluidic pathway of the Vanquish Duo HPLC and UHPLC systems for Inverse Gradient are fitted with Thermo Scientific Viper fingertight fittings, which support near-zero dead volume connections and enable sharper peaks and tool-free setup.

CDS software

The Vanquish Duo systems seamlessly integrate with our Thermo Scientific Chromeleon CDS software to deliver superior compliance tools, networking capabilities, automation and data processing. 


What is inverse gradient liquid chromatography?

The inverse gradient system allows you to consistently quantify all non-volatile and semi-volatile compounds using universal Charged Aerosol Detection.

 

The diagrams below illustrate how an inverse gradient is applied and how varying organic composition effects detector response.

Compared to standard LC, an inverse gradient method adds the inverse of the gradient so the resulting eluent composition is consistent.


Quantification recovery of unknown compounds
 

For a gradient separation, the quantification recovery of unknown compounds with CAD is best with the eluting conditions from the Inverse Gradient approach. For a standard CAD, the quantification recovery will depend on the organic content. UV detection recovery depends on extinction coefficient of the analyte.

Videos


System specifications

The table below provides an overview of the Vanquish Duo HPLC and UHPLC systems for Inverse Gradient specifications for a standard setup. Not all specifications are listed. Your specifications may differ based on the components of your system. Find the specification sheets for the system components in the Component specifications, below.

Category Vanquish Duo HPLC and UHPLC Systems for Inverse Gradient
System pressure, maximum 700, 1000 or 1500 bar, depends on system configuration
Flow range 0-5, 0-8, 0-10 mL/min, depends on system configuration
Pump options (default gradient delay volume)

Binary (35, 200, or 400 µL)

Quaternary (679 µL)

Dual (679 µL)

Delay volume depends on pump(s) selected

Biocompatible Yes, depends on system configuration
pH range

2–12, chloride concentration up to 1 mol/L

 

System design Modular
Touchscreen user interface display compatible (optional) Yes, depends on system configuration
Solvent monitoring system compatible (optional) Yes, depends on system configuration
Customizable gradient delay volume to simplify method transfer Yes, depends on system configuration
SmartInject technology for maximum injection precision and improved column lifetime Yes
Injection volume range

Standard: Up to 0.01-25 or 0.01-100 µL (Vanquish Flex/Vanquish Horizon) 
Optional: Up to 0.01-100 or 0.01-1000 µL (Vanquish Core) 
Depends on system configuration

Sample cooling Yes, 4-40°C 
Depends on sampler selected

Sample capacity (standard)

Based on standard 12 mm OD vials
(≤ 1.5 mL)

4 racks of 54 vials (≤ 1.5mL) each, other rack formats available
+ capacity of 12 x 22.5 mm OD vials (≤ 10 mL) in the carousel
Sampler extension available (optional) Yes, with Vanquish Charger
Column compartment thermostatting

Yes, 5-120°C, depends on system configuration

Still air and forced air

Column compartment pre-heater

Active and passive

Depends on system configuration

Column compartment post-column cooler

Yes

Depends on column compartment selected

Detector choices

Vanquish Charged Aerosol Detector
Optional add-on detectors:
Vanquish Diode Array Detector
Vanquish Multiple Wavelength Detector

Vanquish Variable Wavelength Detector


Component specifications

Standard Vanquish Duo HPLC and UHPLC systems for Inverse Gradient LC are made up of the following components. More detail is available below. 

  Vanquish Horizon Duo UHPLC for Inverse Gradient Vanquish Flex Duo UHPLC for Inverse Gradient Vanquish Core Duo HPLC for Inverse Gradient

Pump

 

Download Specification Sheet

Vanquish Binary Pump H Vanquish Binary Pump F 
or 
Vanquish Quaternary Pump F 
or 
Vanquish Dual Pump F
Vanquish Binary Pump C/CN 
or 
Vanquish Quaternary Pump C/CN 
or 
Vanquish Dual Pump C/CN

Autosampler

 

Download Specification Sheet

Vanquish Split Sampler HT Vanquish Split Sampler FT Vanquish Split Sampler C 
or 
Vanquish Split Sampler CT

Extended sample capacity (optional)

 

Download Specification Sheet

Vanquish Charger  
(optional)

Column compartment

 

Download Specification Sheet

Vanquish Column Compartment H Vanquish Column Compartment C

Virtually zero dead volume system connections

 

Download Specification Sheet

Viper UHPLC Fittings Kits

Detectors

 

Charged Aerosol Detector

For additional detectors, visit our HPLC and UHPLC Detectors page


For Research Use Only. Not for use in diagnostic procedures.