Vanquish Horizon UHPLC System

The Thermo Scientific Vanquish Horizon UHPLC system provides unrivaled performance and throughput for applications requiring high-end UHPLC, ideal for research and development labs. With a fully biocompatible flowpath and ultra-low gradient delay volume, it offers superior separations with unsurpassed retention time and peak area precision, excellent detector sensitivity and lower baseline noise for the most demanding LC and LC-MS workflows. Like all members of our Vanquish product line, Vanquish Horizon UHPLC systems offer hardware precision, detector sensitivity, and simplicity of operation.

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Powerful separations

Vanquish Binary Pump H delivers industry-leading UHPLC flow performance using SmartFlow, SmartStroke and Adaptive Thermal Effect Compensation (ATEC) technologies at maximum operating pressures of up to 1500 bar (22,000 psi). 

Mastering most challenging LC-MS applications

Industry-leading performance results in the highest retention time stability, chromatographic resolution and selectivity. This supports LC-MS workflows using ultra-fast gradients and produces the highest quality data with respect to precision, accuracy and reproducibility.

Automated sample handling

Vanquish Split Sampler HT offers outstanding speed and injection precision using SmartInject technology. It has a default vial capacity of 216 samples, which can be extended up to 8832 samples with the Vanquish Charger sampler extension. 

Sensitive analyte detection

The Vanquish Diode Array Detector HL includes LightPipe technology, featuring a long light path with low dispersion for fast and sensitive detection. We offer additional detection options, including near-universal charged aerosol detection, fluorescence detection and mass spectrometric detection. 

Biocompatible, easy and reliable tool-free connections

The fluidic pathway of the Vanquish Horizon UHPLC system is fully biocompatible and fitted with Thermo Scientific Viper fingertight fittings, which support near-zero dead volume connections and enable sharper peaks and tool-free setup.

CDS software

The Vanquish Horizon UHPLC system integrates with Thermo Scientific Chromeleon Chromatography Data System (CDS) with compliance tools, networking capabilities, automation, and data processing. We offer standard instrument integration (SII) packages to connect Thermo Scientific HPLC systems to other CDS and MS software programs.


System specifications

The table below provides an overview of the Vanquish Horizon UHPLC system specifications for a standard setup. Not all specifications are listed. Your specifications may differ based on the components of your system. Find the specification sheets for the system components in the Component specifications, below.

Category  Vanquish Horizon UHPLC System
System pressure, maximum 1500 bar
Flow range 0–5 mL/min
Pump options (default gradient delay volume) Binary (35 µL)
Biocompatible Yes
pH range  2–12, chloride concentration up to 1 mol/L
System design Modular
Touchscreen user interface display compatible (optional) No
Solvent monitoring system compatible (optional) Yes
Customizable gradient delay volume to simplify method transfer  Yes, with metering device

SmartInject technology for maximum injection precision and improved column lifetime

Injection volume range Standard: 0.01-25 µL
Optional: 0.01-100 µL
Sample cooling

Yes, 4-40°C

Sample capacity (standard)
Based on standard 12 mm OD vials (≤ 1.5 mL)

4 racks of 54 vials  (≤ 1.5 mL) each, other rack formats available

+ capacity of 12 × 22.5 mm OD vials (≤ 10 mL) in the carousel

Sampler extension available

Yes, with Vanquish Charger

Column compartment thermostatting Yes, 5-120 °C 
Still air and forced air 
Column compartment pre-heater Active and passive
Column compartment post-column cooler Yes
Detector choices

Vanquish Charged Aerosol Detector

Vanquish Diode Array Detector

Vanquish Fluorescence Detector

Vanquish Multiple Wavelength Detector

Vanquish Variable Wavelength Detector

Mass Spectrometer

Component specifications

Standard Vanquish Horizon UHPLC systems are made up of the following components. Talk to your local sales rep to discuss your application needs. Download the associated specifications sheet for more detail on each component.

  Vanquish Horizon UHPLC



Download Specification Sheet

Vanquish Binary Pump H



Download Specification Sheet

Vanquish Split Sampler HT

Extended sample capacity (optional)


Download Specification Sheet

Vanquish Charger (optional)

Column compartment


Download Specification Sheet

Vanquish Column Compartment H

Virtually zero dead volume system connections


Download Specification Sheet

Viper UHPLC Fittings Kits



Visit our HPLC and UHPLC Detectors page
Optional components: Fraction Collector Visit our Vanquish Fraction Collector FT page

Contact us to get a quote or discuss an HPLC/UHPLC solution

For Research Use Only. Not for use in diagnostic procedures.