Mycotoxin and Biotoxin Analysis

Identifying harmful natural toxins in food and feed

International trade regulations make testing food and animal feed for biotoxins and mycotoxins a critical step before export. We can help your analytical laboratory comply with global testing requirements and ensure that products meet the necessary standards to ensure toxin-free food products.

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Workflows for mycotoxin analysis

Food and animal feed can become contaminated due to mishandling during processing or the improper application of crop protection chemicals to prevent spoilage of agricultural produce. We can help you economically and confidently test for prevalent phytoxins and mycotoxins including aflatoxins, ochratoxins, patulin, fumonisins, trichothecenes, zearalenone, deoxynivalenol, and others.

Economical quantification of mycotoxins and phytoxins in cereal using LC-MS/MS

With simple, fast sample preparation, this economical and robust method can be used for the quantitation of all 11 legislated mycotoxins worldwide and 37 more that are either already legislated in feed or are prospects for further legislation. The lowest maximum residue levels (MRLs) were reached for all regulated compounds with good precision and reproducibility on five replicates at the limit of detection.

Fast, reliable LC-MS/MS method to quantify mycotoxins in corn-based animal feed

This robust method uses a simple liquid-liquid extraction and a dilute-and-shoot approach to achieve high analyte recovery and reliable quantitation. The method was fully validated in the laboratory four analytical runs with duplicate calibration curves along with 5 quality control sample replicates at low, medium, and high concentrations. The method produced excellent % RSDs for the target analytes over the course of the analytical runs.

Identify and quantify mycotoxins in animal feed using HRAM LC-MS

This method offers an excellent solution for the rapid screening and quantitation of multiple mycotoxin compounds in a single method. The simple QuEChERS extraction procedure from a corn-based animal feed matrix provides an efficient and robust workflow with no further cleanup. All three scan modes studied showed excellent quantitation performance with MS² fragment matching and library searching against highly curated databases.

Fast polarity switching was very effective, with mass accuracies remaining stable across the peak in both polarities, providing plenty of scans across the peak for accurate quantitation. The ability to analyze a sample extract in both positive and negative ion modes, in a single analytical run instead of two, offers a huge productivity advantage.

Quantitative results for Zearalenone in negative mode (A) and positive mode (B) taken from the data dependent acquisition (DDA) scan experiment. Plenty of scans are available across the chromatographic peak (left trace in the figures) with excellent calibration linearity and fragment matches obtained in both polarities.
 Click image to enlargeQuantitative results for Zearalenone in negative mode (A) and positive mode (B) taken from the data dependent acquisition (DDA) scan experiment. Plenty of scans are available across the chromatographic peak (left trace in the figures) with excellent calibration linearity and fragment matches obtained in both polarities.

To help you deliver high quality identification of mycotoxins, we regularly curate our compound and spectral database library for natural toxins to reduce your database development time and cost, while ensuring ultimate confidence and confirmation in the data you report. Though some libraries may have more compounds, these are based on estimated structures developed on lower resolution QTOF instrumentation – lower quality data will give more errors and lower confidence in library searches.

Confirmation of target mycotoxins was performed using MS2 fragment matching and searches against the Thermo Scientific mzCloud highly curated MS2 and MSn data mass spectral database library.

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Related application compendiums


Solutions for mycotoxin analysis

Liquid chromatography and mass spectrometry (LC-MS) workflows enable the analysis of multiple toxins in a single run, with increased confidence and faster turnaround in reportable results, helping you meet your testing demands.

Sample preparation and column chemistry

QuEChERS Dispersive SPE products

  • Complete kits provide a quick, rugged, and cost-effective process for the extraction of multiple mycotoxin analytes.
  • Higher sample recovery and throughput, reduced labor, and lower reagent and solvent costs enable better productivity and lower costs per sample.

Pesticide Explorer: Sample-to-Result LC-MS/MS Pesticides Analytical Workflow

LC-MS and HPLC system comparison

 Vanquish Core HPLC + TSQ Quantis Triple Quadrupole MS
Vanquish Core HPLC + TSQ Quantis Triple Quadrupole MS
Vanquish Flex HPLC + Orbitrap Exploris 120 HRAM MS
Vanquish Flex HPLC + Orbitrap Exploris 120 HRAM MS
Vanquish Flex HPLC + Vanquish Fluorescence Detector
Vanquish Flex HPLC + Vanquish Fluorescence Detector
Fast, multiple toxin single test analysis-
Confirmatory ID
Quantitation
Untargeted quantitation--
Unknown screen & ID--
Sensitive
ThroughputHighHighLow
Quantitation Data analysis software optionsChromeleon CDS
TraceFinder
TraceFinder
Chromeleon CDS
Chromeleon CDS
Unknown characterization and identification data analysis-Compound Discoverer-
Online spectral database solutionmzCloudmzCloud-
Offline spectral database solutionmzVaultmzVault-
= Not applicable = Poor = Good = Better = Best

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