LanthaScreen® Nuclear Receptor Competitive Binding Assay

Our LanthaScreen Nuclear Receptor Competitive Binding Assay contains all the needed reagents you need to conduct compound affinity testing: 

  • Purified nuclear receptor-LBD protein
  • A proprietary fluorescent Fluormone™ ligand
  • An optimized proprietary buffer system
  • Terbium-labeled anti-GST antibody

The nuclear receptor (NR) protein is indirectly labeled with terbium via the anti-GST antibody bound to the GST tag on the receptor. When this labeled protein binds to the Fluormone™ ligand, a high TR-FRET ratio will result. If a test compound that binds to the NR protein is added to the reaction, the test compound will compete with the Fluormone™ ligand, resulting in a lower TR-FRET ratio. Since the change in TR-FRET ratio only occurs in the presence of a competitor, it can be used as a convenient and accurate indicator of the relative affinity of the test compound for the NR (Figure 1 below).

Figure 1. Assay schematic of a Nuclear Receptor LanthaScreen®

Selection Table for LanthaScreen® Nuclear Receptor Competitive Binding Assays (TR-FRET)

Target Alias(es) Accession number LanthaScreen® Competitive Binding Assay (TR-FRET)
Estrogen receptor alpha ER Alpha, ERA, ESR1, NR3A1 NP_000116.2 A15887
Estrogen receptor beta ER Beta, ERB, ESR2, NR3A2 NP_001428.1 A15895
Glucocorticoid receptor GR, GCCR, GCR, NR3C1 NP_000167 A15901
Peroxisome proliferator-activated receptor alpha PPAR Alpha, PPARA, NR1C1 NP_005027 PV4892
Peroxisome proliferator-activated receptor delta PPAR Delta, PPARD, NR1C2 NP_006229 PV4893
Peroxisome proliferator-activated receptor gamma PPAR Gamma, PPARG, NR1C3 NP_005028 PV4894
Pregnane X receptor PXR(SXR), NR1I2 NP_003880.3 PV4839
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
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