Artist rendering of DNA molecules

To support the manufacturing of cell and gene therapies, we have developed innovative chromatography solutions, specifically designed to improve the downstream purification of viral vectors such as adeno-associated virus (AAV), lentivirus (LV), and adenovirus 5 (Adv5).

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Benefit from:

  • High selectivity and capacity for the purification of viral vectors
  • An efficient and robust purification process, from crude material in one step, offering scalability and process consistency
  • Significant improvement to the downstream process of viral vectors, by reducing the purification steps and maximizing productivity

Lentiviral Particle

AAV purification

Our proven CaptureSelect ligand technology, combined with the large pore structure of the POROS beads, enables efficient purification of large biomolecules such as viral vectors. These unique affinity resins are developed to significantly improve downstream processing of viral vectors by reducing the number of purification steps and offering scalability, thus maximizing process consistency, efficiency, and productivity.

POROS CaptureSelect AAV resins are unique affinity chromatography resins for the purification of adeno-associated virus (AAV) used in gene therapy applications.

Key benefits:

  • High dynamic binding capacity and superior resolution address capacity and selectivity requirements for purifying viral vectors
  • Robust viral clearance capabilities of the resins help to reduce the risk of possible viral contamination derived from cell culture production

When the use of your product requires further polishing, such as removal of empty capsids, Thermo Scientific Ion Exchange resins can be utilized for subsequent polishing steps. The excellent resolution of these resins allows for separation of closely related product forms such as empty and full viral capsids.

All AAV affinity purification and polishing resins come with full regulatory support, enabling use in commercial manufacturing.

For more information and products in the AAV manufacturing workflow, please visit our page on AAV production.

Table 1. Summary of POROS CaptureSelect AAV affinity resin properties

 

Binding capacity (vg/mL)*

Serotype affinity

POROS CaptureSelect AAVX

>1014

AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, recombinant and chimeric vectors**

POROS CaptureSelect AAV9

>1014

AAV9

POROS CaptureSelect AAV8

>1013

AAV8

*Measured as viral genomes per milliliter (vg/mL).
**The AAVX Affinity ligand has demonstrated binding reactivity towards a large set of AAV serotypes (including chimeric vectors), suggesting that an even broader range of AAV serotypes might be purified by this resin.


Resins and reagents for AAV purification


Lentivirus purification solutions

Our Lentivirus purification solutions are developed to help improve the downstream process efficiency of this group of viral vectors.

CaptureSelect Lenti-VSVG affinity matrix

This novel affinity matrix has been designed specifically for the purification of VSV-G pseudotyped lentivirus particles. The Lenti-VSVG affinity matrix offers an efficient purification method combined with gentle elution conditions, which helps to retain infectivity of these particles.

The Lenti-VSVG affinity matrix is a research use only (RUO) chromatography resin but will be further developed into a bioprocess resin suitable for cGMP manufacturing. For more details, please fill out the contact form.

POROS Heparin affinity resin

Heparin affinity chromatography is a simple and effective way of purifying proteins containing a heparin binding domain. Due to its important biological function, the domain is often readily accessible, allowing for efficient purification. POROS 50 HE Heparin Affinity resin can be used for the purification of viral vectors containing a Heparin binding site, such as lentivirus or baculovirus.

POROS D50 weak anion exchange resin

Anion exchange chromatography can serve as an ideal solution for the purification of lentiviral particles. The features of POROS D50 resin, a weak anion exchanger, make it suitable for lentivirus capture.


Resins and reagents for lentivirus purification


Adenovirus-5 (AdV5) purification

To support the research and development of adenoviral vectors, a resin for the purification of adenovirus serotype 5 (AdV5) was developed. POROS CaptureSelect AdV5 Affinity Matrix is specifically designed for the purification and/or scavenging of recombinant hexon capsomeres of AdV5 particles, as well as free hexon proteins. The AdV5 affinity matrix is a research use only (RUO) chromatography resin but can be further developed into a bioprocess resin suitable for cGMP manufacturing.

Viral Safety in AAV Production: How Affinity Chromatography Effectively Contributes

Bioprocessing resources

Access a targeted collection of scientific application notes, case studies, posters, white papers and more for bioprocessing:

For research use or further manufacturing, not for diagnostic use or direct administration in humans or animals.