Affinity Resins—Biosimilars and Recombinant Proteins

We have developed a unique portfolio of affinity resins to help you develop biosimilars, biobetters, and other types of recombinant proteins. These affinity resins can be used for clinical and commercial production.

CaptureSelect Affinity resin benefits:

High Selectivity─high purity in a single capture step, independent of feedstock
Mild elution conditions─retention of biological activity of target
Reduction of process steps─helps reduce costs, allows higher yields
Efficient clearance of impurities

In addition to affinity resins, we also offer product-specific ELISAs to measure any ligand potentially leaching from the column.

The graphic below depicts a typical workflow in which CaptureSelect resin can be used for a primary capture step for the efficient production of recombinant proteins.

CaptureSelect affinity matrices:

Product	Target applications	Uniqueness	RoboColumn™ format
CaptureSelect Follicle Stimulating Hormone (FSH)	Human follicle stimulating hormone	Binds only intact FSH due to alpha/beta chain epitope
CaptureSelect Human Albumin	Human serum albumin, albumin fusion proteins	Mild elution conditions help to protect fusion partner	X
CaptureSelect hCG	Binds to the alpha chain of hCG (human chorionic gonadotropin)	Selective for hCG, with cross-binding to LH, FSH, and TSH
CaptureSelect hGH	Binds recombinant human Growth Hormone	High yield and retained activity
CaptureSelect tPA	Human tissue plasminogen activator (tPA)	The only tPA affinity resin available on the market
CaptureSelect TSH	Human Thyroid Stimulating Hormone	Selective for TSH with no cross binding to hormones of the Gonadotropin family
CaptureSelect C-tagXL	Binds the short C-tag sequence E-P-E-A (glutamic acid - proline - glutamic acid – alanine) when this affinity tag is fused at the C-terminus of a target protein	Optimized to obtain high yields in protein purification

Quantitate the yield of your proteins during the production process with our analytical columns or develop your own analytical assay with CaptureSelect Ligand Conjugates.

When your specific process needs cannot be met with current products, our custom chromatography services are available to build you a unique solution. For more information, please visit Custom Capabilities .

CaptureSelect Leakage ELISAs

Products incorporating CaptureSelect technology are used for clinical and commercial manufacturing of a variety of biologicals. To support these products, Thermo Fisher Scientific has developed product-specific Leakage ELISAs that can be used for routine quality analysis. Below is a list of Leakage ELISAs and their corresponding affinity resins.

Product	Complementing resin
CaptureSelect FSH Ligand Leakage ELISA	CaptureSelect Follicle Stimulating Hormone (FSH) Affinity Matrix
CaptureSelect HSA Ligand Leakage ELISA	CaptureSelect Human Albumin Affinity Matrix
CaptureSelect hGH Ligand Leakage ELISA	CaptureSelect hGH Affinity Matrix
CaptureSelect hCG Ligand Leakage ELISA	CaptureSelect hCG Affinity Matrix
CaptureSelect tPA Ligand Leakage ELISA	CaptureSelect tPA Affinity Matrix
CaptureSelect TSH Ligand Leakage ELISA	CaptureSelect TSH Affinity Matrix
CaptureSelect C-tagXL Ligand Leakage ELISA	CaptureSelect C-tagXL Affinity Matrix
CaptureSelect tPA Ligand Leakage ELISA	CaptureSelect tPA Affinity Matrix
CaptureSelect VII Select Leakage ELISA Kit	VIISelect *
CaptureSelect VIII Select Leakage ELISA Kit	VIIISelect *
CaptureSelect IX Select Leakage ELISA Kit	IXSelect *
CaptureSelect GCSFSelect Leakage ELISA Kit	GCSFSelect *
CaptureSelect Alpha-1 Antitrypsin Select Leakage ELISA Kit	Alpha-1 Antitrypsin Select *

* Complementing resins are available through GE Healthcare Life Sciences

Articles citing CaptureSelect technologies:

Jing Jin (2018) Production, quality control, stability, and potency of cGMPproduced Plasmodium falciparum RH5.1 protein vaccine expressed in Drosophila S2 cells
McCue JT, Selvitelli K, Walker J (2009) Application of a novel affinity adsorbent for the capture and purification of recombinant factor VIII compounds. J Chromatogr A 1216(45):7824–7830.
Casademunt E, Martinelle K, Jernberg M et al. (2012) The first recombinant human coagulation factor VIII of human origin. Eur J Haematol 89(2):165–176.
Detmers F, Mueller F, Rohde J (2013) Increasing purity and yield in biosimilar production. BioProcess International, June 2013.