Protein A chromatography relies on the specific binding of antibodies to an immobilized Protein A ligand. Native Protein A or surface Protein A (SPA), is a 55-kDa bacterial cell wall-derived component that naturally binds to IgG molecules.
Modifications to the first generation of Protein A ligands has given way to highly robust and caustic stable purification resins that allow you to design antibody purification processes with greater flexibility. Thermo Fisher Scientific offers Protein A chromatography resins which are designed to help you improve the efficiency and productivity of your antibody purification process.
Native Protein A has 5-homologous binding domains (A-E) that fold into a 3-helix bundle; each of which bind to proteins across a range of mammalian species, most notably human IgG. Long regarded as the platform of choice for antibody purification, Protein A binds to IgG1, IgG2 and IgG4 class molecules and has affinity towards:
Thermo Fisher Scientific offers various Protein A affinity resins constructed of different ligands and bead matrices. These resins are built to:
Designed for high productivity, featuring a highly cross-linked agarose-based backbone. The engineered Protein A affinity ligand on this resin is equipped with:
Access a targeted collection of scientific application notes, case studies, posters, white papers and more for bioprocessing:
Pharmaceutical Grade Reagent. For Manufacturing and Laboratory Use Only.