ViewRNA Cell Plus Assay

Ultra-Sensitive RNA and Protein Detection

ViewRNA ISH Cell and Cell Plus Assays utilize RNA FISH, a powerful technique that combines fluorescence ISH (FISH) and sequential branched-DNA (bDNA) amplification technique to visualize RNA with single-molecule sensitivity along with protein in individual cells. ViewRNA ISH Cell Assay (RNA detection) and Cell Plus Assays (RNA and protein detection) are increasingly important for tracking RNA and protein and understanding the relationship between transcription and translation at the single cell level–technologies that are fundamental to a viral research and a broad range of biological application areas.

Order your ViewRNA ISH Cell or Cell Plus Assay  Find SARS-CoV-2 probes

ViewRNA ISH Cell and Cell Plus Assays offer:

  • Visualization of RNA (ISH Cell Assay) or simultaneous visualization or RNA and protein (Cell Plus Assay) through fluorescence in situ hybridization
  • Single mRNA molecule detection without RNA isolation, reverse transcription, or PCR amplification
  • Visualization of targets not easily localized using other technologies (see also ViewRNA Tissue Assay for hard to work with tissue samples)

On this page:

ViewRNA ISH Cell Assays using bDNA technology

There are four parts to the ViewRNA ISH Cell and Cell Plus assay workflow (Figure 1). In the first step, the sample is fixed, permeabilized, and prepared to allow target accessibility (Note: For ViewRNA Cell Plus Assay antibody staining should occur prior to in situ hybridization. It is recommended to use antibodies that have been tested for assay compatibility using ViewRNA Cell Plus Fixation/Permeabilization Buffer Set–see table below for antibodies that have been tested for compatibility). In Step 2, a proprietary, target-specific Probe Set of approximately 20 oligonucleotide pairs hybridizes to each target mRNA (Table 1). Signal amplification is achieved through specific hybridization of adjacent oligonucleotide pairs to bDNA structures (Step 3), which provides much higher specificity of signal than traditional Stellaris FISH assays. ViewRNA Assays have proven successful for detection of RNA with a length as small as 20 nucleotides, which is particularly useful when target RNA length is very small, such as microRNA, circular RNA, splice variants, etc.

Figure 1. Overview of ViewRNA ISH Cell Assay workflow.

Overview of ViewRNA ISH cell assay workflow
 Click image to enlarge

Signal amplification (Step 3) using bDNA technology is achieved via a series of sequential hybridization steps that require Pre-Amplifier molecules, Amplifier molecules and Fluorescent Label Probes that all hybridize to each other form a fully assembled signal amplification 'tree', which has ~400 binding sites for each Label Probe, resulting in an ~8,000-fold amplification for each individual transcript. Target mRNAs are visualized and detected (Step 4) using a standard fluorescent microscope such as an EVOS Cell Imaging System or with the required filter sets (see Ordering Information, Step 1).  For accurate and quantitative detection of ViewRNA spots per cell, or intensity of spots, a high-content imager such as a CellInsight High-Content Screening Instrument can also be used for 96-well, 384-well, or 1536-well plate high-content format.

Note for ViewRNA Cell Plus Assay: As with ViewRNA ISH Cell assay, the ViewRNA Cell Plus assay uses ISH bDNA technology, but has been formatted and optimized for use with antibody-based protein detection to simultaneously visualize RNA and protein in individual cells. The ViewRNA Cell Plus assay enables detection of up to four RNA targets in combination with immunophenotyping for cell surface or intracellular proteins using both indirect and direct immunocytochemistry. Antibody compatibility with this assay must be determined using either the ViewRNA Cell Plus Fixation/Permeabilization Buffer Set–see table below for antibodies that have been tested for compatibility. As with any immunocytochemistry experiment, antibodies should be titrated for optimal performance.

Table 1. Probe sets available for ViewRNA ISH Cell and Cell Plus Assays.

Probe set Fluorescent label ViewRNA ISH Cell Assay compatibility
Type 1 Alexa Fluor 546  ISH Cell and Cell Plus Assays
Type 4 Alexa Fluor 488 ISH Cell and Cell Plus Assays
Type 6 Alexa Fluor 647  ISH Cell and Cell Plus Assays
Type 10 Alexa Fluor 750 ISH Cell and Cell Plus Assays

 

ViewRNA ISH Cell and Cell Plus Assays at-a-glance

  ISH Cell Assay Cell Plus Assay
Use
  • Detect single RNA molecules at single-cell resolution and quantify gene expression with a limit of detection of single RNA.
  • Visualization of RNA (Cell Assay) or RNA and protein (Cell Plus Assay) through fluorescence in situ hybridization
  • Detection of Micro-RNA, circular RNA, exon junctions/splice variants, gene fusion, gene knockout, pre-miRNA, gene editing/CRISPR, CAR-T cell validation and detection
Differences Optimized for cultured cells or circulating tumor cells (CTCs) Compatible with multiplex mRNA detection by immunocytochemistry (immunostaining)
Sample type Optimized for detection of mRNA in cell culture and circulating tumor cells (CTCs)
  • Compatible with detection of mRNA in cell culture and circulating tumor cells (CTCs)
  • Compatible with tissue and embryo, with minor modifications to the protocol
Compatible
  • Coverslips mounted on slides 
  • 96-well compatible
  • High content kits available for 384-well plates
  • Coverslips mounted on slides 
  • 96-well compatible
  • ICC/IF compatible (1 to 2-plex protein)
Not compatible Not ICC/IHC compatible Not compatible with 384-well plates
Plex level and fluorophore options
  • 1 to 4-plex RNA
     
  • Alexa Fluor 488
  • Alexa Fluor 546
  • Alexa Fluor 647
  • Alexa Fluor 750
  • 1 to 4-plex RNA and one or more protein targets, or any combination of RNA and protein as long as the fluorescence readout is optically distinct
     
  • Alexa Fluor 488
  • Alexa Fluor 546
  • Alexa Fluor 647
  • Alexa Fluor 750
Instrumentation
  • Hybridization oven
  • Fluorescence or microscope/scanner
  • Hybridization oven
  • Fluorescence or microscope/scanner

Need to assay tissue samples? A separate kit has been developed for assaying tissue. Learn more about the ViewRNA ISH Tissue Assay which can be run as a chemiluminescent or fluorescent assay.
 

Featured ViewRNA ISH Cell Assay applications

Single RNA molecule visualization using ViewRNA ISH Cell Assay

Visualize single RNA molecules at the single cell level

Simultaneous visualization of protein and RNA targets with ViewRNA Cell Plus

Simultaneous visualization of protein and RNA targets with ViewRNA Cell Plus


Combining FISH and ICC with the ViewRNA Cell Plus Assay

Combining FISH and ICC with the ViewRNA Cell Plus Assay

Identification of breast cancer CTCs using the ViewRNA ISH Cell Assay

Figure 1. Identification of breast cancer CTCs from human bone marrow using the ViewRNA ISH Cell Assay. A putative breast cancer tumor cell from a bone marrow sample was fixed and analyzed using the ViewRNA ISH Cell Assay for ERBB2 (green), pan-keratin panel (cytokeratin 8, 18, and 19) (yellow), and BACT (purple). The technology was used to identify the only two such CTCs on the slide. Data courtesy of Radium Hospital, Oslo, Norway.

HeLa

Visualization and quantitation of transcript heterogeneity in cell lines using the ViewRNA ISH Cell Assay

SKBR3

Visualization and quantitation of transcript heterogeneity in cell lines using the ViewRNA ISH Cell Assay

Figure 2. Visualization and quantitation of transcript heterogeneity in cell lines using the ViewRNA ISH Cell Assay. Multiplex analysis of Her2 mRNA (green) and control 18S rRNA (red) in HeLa cells and SKBR3 cells. Nuclei were stained with DAPI (blue) and visualized by fluorescence microscopy.
 

Time course of gene expression at the single-cell level using the ViewRNA ISH Cell Assay

Figure 3. Time course of gene expression after cytokine induction, at the single-cell level using the ViewRNA ISH Cell Assay. HeLa cells were treated with PMA for 0–8 hours. Then the cells were fixed and analyzed using the ViewRNA ISH Cell Assay for induction of IL-6 (red) and IL-8 expression (green). Nuclei were stained with DAPI (blue). The results show post-induction peaks at 1–2 hours for IL-6 and 4 hours for IL-8. Furthermore, the assay reveals the heterogeneity of individual cell responses.

Detection of H1N1 influenza A RNA in cells using the ViewRNA ISH Cell Assay

Figure 4. Detection of H1N1 Influenza A RNA migration in cells using the ViewRNA ISH Cell Assay. Nuclear translocation of viral RNA is necessary for replication of the influenza virus. Murine embryonic fibroblasts (MEFs) were incubated on ice with influenza A virus (H1N1, PR8 strain). At time 0, virus was removed and medium (warmed to 37°C) was added. At the indicated time points after warming, the cells were fixed and processed using the ViewRNA ISH Cell Assay Kit with a probe set against the nucleoprotein (NP) viral genomic segment (green). Nuclei are stained blue with DAPI. Time dependent nuclear translocation of the NP genome is seen at 90 min post-infection, and by 180 min the export of newly synthesized viral genomes from the nuclei to the cytosol is also observed. Data courtesy of Dr. Abraham L Brass, Ragon Institute of MGH, MIT, and Harvard.

Multiplexing of viral RNA and mRNA in Huh7 cells using View RNA Assays

Figure 5. Multiplexing of viral RNA and mRNA in Huh7 cells. Multiplex fluorescence in situ detection of HCV genomic viral RNA (green) and 18S rRNA (red) in Huh7 cells lacking (–HCV) or containing (+HCV) a hepatitis C virus (HCV) replicon. Nuclei are stained with DAPI (blue).

Multiplex analysis of miRNA and mRNA expression levels using the ViewRNA Cell Assay.

Figure 6. Multiplex analysis of miRNA and mRNA of various expression levels using the ViewRNA Cell Assay. Simultaneous detection of miRNA (Fast Red substrate, Cy3/550 channel) and mRNA (in FITC channel or in Cy7 channel) expression in any given cell. Shown are duplex assays showing expression of different miRNA and mRNA targets in HeLa cells. HPRT, ACTB, and PPIB were detected in the FITC channel, and GAPD was detected in the Cy7 channel. Based on known expression levels, the data confirm accurate and precise study and visualization of miRNA and mRNA of various expression levels, where one distinct spot corresponds to one molecule of an miRNA or mRNA target.

Compound dose-response profiling

Visualization and quantitation of cultured cells using the ViewRNA Cell Assay for high-content screening

Figure 7. Visualization and quantitation of induction in cultured cells using the ViewRNA Cell Assay for high-content screening. HeLa cells were cultured in a 384-well clear bottom plate and treated with various concentrations of PMA. Following a 4-hour PMA treatment, cells were fixed and analyzed for IL-8 expression (green). Nuclei were stained with DAPI (blue).

Compound screening with multiple markers

Multiplex analysis of compound screens using the ViewRNA Cell Assay for high-content screening

Figure 8. Multiplex analysis of compound screens using the ViewRNA Cell Assay for high-content screening. Individual images show nuclei and RNA targets: gene 1, gene 2, and gene 3. The merged image shows simultaneous detection of the three RNA targets. Nuclei were stained with DAPI (blue). Data courtesy of Dr. Dan Garza, Proteostasis, Inc.

Ordering information

Follow the five steps below to begin your ViewRNA ISH Cell or Cell Plus Assay

Instrument Specification
Fluorescent microscope or high-content screening instrument

Dependent upon fluorophores used in your experiment. Recommendations per fluorophore are listed

Alexa Fluor 488
Ex/Em: 490/525
Suggested filter: FITC

Alexa Fluor 546
Ex/Em: 556/573
Suggested filter: TRITC 
Alexa Fluor 647
Ex/Em: 650/665
Suggested filter: Cy5
Alexa Fluor 750
Ex/Em: 740/775
Suggested filter: Cy7
Incubator Capable of maintaining temperature at 40 ± 1°C
Metal heat block For 1.5 mL microcentrifuge tube, placed inside the validated incubator
Temperature validation kit Use a calibrated thermometer to verify the incubator temperature

Instructions: Choose one assay from the two available kits in step 1. Following selection in step 1 choose any accessory kits and reagents that might be needed for the chosen assay. Make sure to select from the appropriate table of accessory kits and reagents for the chosen assay.

  • A maximum of four types of probe sets are currently available for RNA detection.
  • Select different Probe Sets depending on the RNA expression level and kit selected.
  • For multiplex analysis with immunolabeling of both cell-surface and intracellular proteins, find fluorophore-conjugated antibodies.
Probe Set type Expression level of target RNA Fluorochrome label Laser excitation wavelength (nm) Suggested bandpass filter (nm)
Type 1 Low Alexa Fluor 546 561 610/20
Type 4 Medium-to-high Alexa Fluor 488 488 530/30
Type 6 Low Alexa Fluor 647 633, 635 660/20
Type 10 Medium-to-high Alexa Fluor 750 633, 635 780/60

Search for available (in stock) Probe Sets

Custom Branched DNA Probe Set Tool

Not finding what you’re looking for? Order Custom Branched DNA Probe Sets for PrimeFlow, QuantiGene Singleplex, and ViewRNA Cell and Tissue assays using the custom ordering tool if your target of interest is not found in our web catalog.

Customer bDNA Probe Set Ordering Tool

  • Positive control—use positive control probe sets in every experiment to ensure proper assay performance: Housekeeping genes such as ACTB, GAPD, PPIB or UBC probe sets are commonly used as positive controls.  For micro-RNA experiments, probe set for Let7a works for many sample types. 
  • Negative control—such as samples with the target specific probe omitted, or samples labeled with a probe against a target not expressed in the cells of interest (e.g., dapB, a bacterial gene); highly recommended.
  • Search probes used for positive and negative controls in probe search tool in Step 4, for the species.  Please note that ViewRNA probes are very specific, and probe set for one species, commonly does not detect targets in another species.

Antibody compatibility for ViewRNA Cell Plus Assay

We have evaluated the performance of more than 100 ICC-compatible antibodies (comprised of a mix of unconjugated/purified, biotinylated, and fluorophore-conjugated format; see table below) for use in ViewRNA Cell Plus Assay. To accomplish this, antibody performance was tested either in the context of the full assay (antibody staining and RNA detection) or by using Invitrogen ViewRNA Cell Plus Fixation/Permeabilization Buffer Set
(Cat. No. 00-19001) according to the antibody staining portion of the protocol. We found that 95% of the antibodies tested that perform optimally with formaldehyde-fixed cells also perform well with the ViewRNA Cell Plus Assay. Antibodies that require methanol fixation were generally found to be unsuitable for this assay.

In this evaluation, we have assessed a broad panel of antibodies with specificities against structural proteins, transcription factors, organelles, and surface markers. Our results show that signal intensity and resolution of detail is maintained with ViewRNA Cell Plus Assay compared to our standard immunocytochemistry protocol. Based on our studies, we conclude that antibodies which are suitable for ICC or immunohistochemistry (IHC) (i.e., optimized for formaldehyde fixation) are likely to be compatible with ViewRNA Cell Plus Assay. Nevertheless, we recommend using ViewRNA Cell Plus Fixation/Permeabilization Buffer Set (Cat. No. 00-19001) to establish acceptable performance criteria prior to running the complete ViewRNA Cell Plus Assay.

Species Specificity Clone Cat. No. Conjugate Concentration tested
Recommended
Human, Mouse, Rat, Canine, Bovine, Guinea Pig, Pig, Horse Actin HHF35 14-6496 Purified 10 µg/mL
Human, Mouse, Rat, Canine, Bovine, Guinea Pig, Pig, Horse Actin HHF35 53-6496 Alexa Fluor 488 30 µg/mL
Human AFP (alpha fetoprotein) 1E8 14-9499 Purified 5 µg/mL
Human AHR FF3399 14-9854 Purified 20 µg/mL
Human ALDH1L1 7G8 14-9595 Purified 1 µg/mL
Human alpha-Catenin 1G5 MA1-2000 Purified 5 µg/mL
Human Arginase-1 sl6arg 53-9779 Alexa Fluor 488 20 µg/mL
Human Arginase-1 sl6arg 50-9779 eFluor 660 5 µg/mL
Human Arginase-1 sl6arg 14-9779 Purified 5 µg/mL
Human, Mouse B220 RA3-6B2 53-0452 Alexa Fluor 488 20 µg/mL
Human, Mouse, Rat, Bovine Beta tubulin class III 2G10-TB3 14-4510 Purified 5 µg/mL
Human, Mouse, Rat, Bovine Beta tubulin class III 2G10-TB3 53-4510 Alexa Fluor 488 10 µg/mL
Human, Mouse, Rat, Bovine Beta tubulin class III 2G10-TB3 50-4510 eFluor 660 10 µg/mL
Human, Mouse, Rat, Bovine Beta tubulin class III 2G10-TB3 41-4510 eFluor 570 5 µg/mL
Human Brachyury X1AO2 14-9770 Purified 5 µg/mL
Mouse CD3 17A2 50-0032 eFluor 660 10 µg/mL
Human, Mouse and Canine CD324, E-Cadherin DECMA-1 53-3249 Alexa Fluor 488 20 µg/mL
Mouse CD4 RM4-5 13-0042 Biotin 10 µg/mL
Mouse CD4 RM4-5 41-0042 eFluor 570 5 µg/mL
Mouse CD8 53-6.7 50-0081 eFluor 660 10 µg/mL
Mouse CD8a 4SM15 13-0808 Biotin 10 µg/mL
Mouse CD11b M1/70 50-0112 eFluor 660 5 µg/mL
Human, Mouse, Rat, Chicken Connexin 32 2A 14-9759 Purified 5 µg/mL
Human Cox IV IB52C31H10 14-9775 Purified 1 µg/mL
Human CoxIV IB52C31H10 53-9775 Alexa Fluor 488 10 µg/mL
Human Cytokeratin 7 LP5K 14-9005 Purified 10 µg/mL
Human, Mouse, Rat Early endosome antigen 1 1G11 14-9114 Purified 1 µg/mL
Human CD326 EpCAM 1B7 14-9326 Purified 10 µg/mL
Human ER alpha tbykt 14-9740 Purified 1 µg/mL
Human Fibronectin FN-3 14-9869 Purified 10 µg/mL
Human, Mouse, Rat, Pig GFAP GA5 53-9892 Alexa Fluor 488 10 µg/mL
Human Golgin-97 CDF4 14-9767 Purified 1 µg/mL
Human, Mouse, Rat Grim-19 1A8 14-9937 Purified 5 µg/mL
Human Her2 MJD2 14-9757 Purified 5 µg/mL
Human, Mouse Her4 HFR1 14-9687 Purified 10 µg/mL
Human Ki-67 20Raj1 42-5699 eFluor 615 1 µg/mL
Human Ki-67 20Raj1 50-5699 eFluor 660 20 µg/mL
Human Ki-67 20Raj1 11-5699 FITC 10 µg/mL
Human Ki-67 20Raj1 14-5699 Purified 1 µg/mL
Human Ki-67 20Raj1 13-5699 Biotin 5 µg/mL
Human Ki-67 20Raj1 41-5699 eFluor 570 5 µg/mL
Mouse, Rat Ki-67 SolA15 41-5698 eFluor 570 0.5 µg/mL
Mouse, Rat Ki-67 SolA15 50-5698 eFluor 660 10 µg/mL
Mouse, Rat MASH1 24B72D11 14-5794 Purified 20 µg/mL
Human, Mouse, Rat, Dog Myogenin F5D 53-5643 Alexa Fluor 488 5 µg/mL
Human, Mouse, Rat, Dog Myogenin F5D 14-5643 Purified 1 µg/mL
Human, Mouse, Rat, etc. Myosin MF20 53-6503 Alexa Fluor 488 5 µg/mL
Human, Mouse, Rat, etc. Myosin MF20 50-6503 eFluor 660 10 µg/mL
Mouse Nanog eBioMLC-51 50-5761 eFluor 660 10 µg/mL
Mouse Nanog eBioMLC-51 53-5761 Alexa Fluor 488 10 µg/mL
Human Nestin 10C2 53-9843 Alexa Fluor 488 10 µg/mL
Human Nestin 10C2 14-9843 Purified 5 µg/mL
Human, Mouse OCT3/4 EM92 41-5841 eFluor 570 5 µg/mL
Human, Mouse, Rat PCNA PC10 14-9910 Purified 1 µg/mL
Human, Mouse, Rat Phospho-ATM 10H11.E12 14-9046 Purified 10 µg/mL
Human, Mouse Phospho-ERK1/2 MILAN8R 14-9109 Purified 5 µg/mL
Human, Mouse Phospho-H2AX CR55T33 14-9865 Purified 5 µg/mL
Human Progesterone receptor KMC912 13-9764 Biotin 2.5 µg/mL
Human Progesterone receptor KMC912 14-9764 Purified 1 µg/mL
Human Progesterone receptor KMC912 50-9764 eFluor 660 20 µg/mL
Human Snail1 20C8 53-9859 Alexa Fluor 488 20 µg/mL
Human Snail1 20C8 14-9859 Purified 5 µg/mL
Human, Mouse Sox2 Btjce 41-9811 eFluor 570 5 µg/mL
Human, Mouse Sox2 Btjce 50-9811 eFluor 660 10 µg/mL
Human, Mouse Sox2 Btjce 14-9811 Purified 5 µg/mL
Human, Mouse Sox9 GMPR9 53-9765 Alexa Fluor 488 10 µg/mL
Human, Mouse Sox9 GMPR9 14-9765 Purified 1 µg/mL
Human Survivin STLALYV 53-9176 Alexa Fluor 488 10 µg/mL
Human, Mouse, Rat Thrombospondin-1 A6.1 14-9756 Purified 1 µg/mL
Human, Mouse, Rat, etc. Tubulin DM1A 42-4502 eFluor 615 1 µg/mL
Human, Mouse, Rat, etc. Tubulin DM1A 53-4502 Alexa Fluor 488 5 µg/mL
Human, Mouse, Rat, etc. Tubulin DM1A 14-4502 Purified 5 µg/mL
Human, Mouse, Rat, etc. Tubulin DM1A 13-4502 Biotin 10 µg/mL
Human, Rat, Chicken, Canine Vimentin V9 41-9897 eFluor 570 1 µg/mL
Human, Rat, Chicken, Canine Vimentin V9 50-9897 eFluor 660 5 µg/mL
Human, Mouse, Rat, Avian Vinculin 7F9 41-9777 eFluor 570 10 µg/mL
Human, Mouse, Rat, Avian Vinculin 7F9 53-9777 Alexa Fluor 488 20 µg/mL
Human, Mouse, Rat, Avian Vinculin 7F9 14-9777 Purified 5 µg/mL
Mouse, Rat, Canine ZO-1 R26.4C 41-9776 eFluor 570 1 µg/mL
Human CD3 OKT3 50-0037 eFluor 660 0.12 µg/ml
Human CD4 N1UG0 41-2444 eFluor 570 20 µg/mL
Mouse FoxP3 FJK-16s 53-5773 Alexa Fluor 488 10 µg/mL
Not recommended
Human, Mouse B220 RA3-6B2 50-0452 eFluor 660 10 µg/mL
Mouse CD4 GK1.5 53-0041 Alexa Fluor 488 10 µg/mL
Mouse CD4 GK1.5 50-0041 eFluor 660 5 µg/mL
Mouse, Rat Nestin Rat-401 14-5843 Purified 10 µg/mL
Mouse, Human, Rat, etc. Cytokeratin AE1/AE3 42-9003 eFluor 615 5 µg/mL
Mouse, Human, Rat, etc. Cytokeratin AE1/AE3 41-9003 eFluor 570 1 µg/mL
Mouse, Human, Rat, etc. Cytokeratin AE1/AE3 53-9003 Alexa Fluor 488 1 µg/mL
Human, Mouse, Rat, Chicken Connexin 32 2A 53-9759 Alexa Fluor 488 1 µg/mL
Human, Mouse, Rat Connexin 43 1A 14-4759 Purified 5 µg/mL
Human Heat shock protein 27 STRSN 14-9112 Purified 1 µg/mL
Mouse MHC Class II M5/114.15.2 11-5321 FITC 20 µg/mL
Human, Mouse, Rat PCNA PC10 42-9910 eFluor 615 0.5 µg/mL
Human CD8 AMC908 50-0008 eFluor 660 5 µg/mL

Do you need SARS-CoV-2 probes?

Use the step-by-step ordering information above to order your kit and probes, including controls. In step 4, if you need SARS-CoV-2 probes, see the table below, and follow the ‘How to order SARS-CoV-2 probes’ instructions provided.

Dye Type Scale Catalog
number
Assay ID
AF546 1 120 μL CVX-06 VPNKRHH
AF546 1 440 μL CVX-01 VPNKRHH
AF546 1 1440 μL CVX-02 VPNKRHH
AF488 4 120 μL CVX-06 VP9HHYZ
AF488 4 440 μL CVX-01 VP9HHYZ
AF488 4 1440 μL CVX-02 VP9HHYZ
AF647 6 120 μL CVX-06 VPAAADR
AF647 6 440 μL CVX-01 VPAAADR
AF647 6 1440 μL CVX-02 VPAAADR
AF750 10 120 μL CVX-06 VPCE3XN
AF750 10 440 μL CVX-01 VPCE3XN
AF750 10 1440 μL CVX-02 VPCE3XN

ViewRNA ISH Cell and Cell Plus Probe Sets were designed using the NCBI strain and sequence from Accession Number LC521925, and cover multiple regions of the viral transcript. Find in the table below the binding sites and transcript region for the probe sets included in the assay. If you need a custom probe designed for a specific RNA region use the Custom Branched DNA Probe Set Tool.

  Position (5' to 3') Region
1 705-1676 orf1ab
2 4356-5607 orf1ab
3 12808-13942 orf1ab
4 20571-21759 orf1ab
5 26646-27763 M
6 28212-29152 orf8 and N
Related products

Resources

Support