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Competent Cells Packaging Formats
Each project has its own unique requirements. To meet these different demands, many of Invitrogen’s competent cells are offered in a variety of packaging formats. Custom formats are also available.
Competent cells are available in a variety of formats
| Format / Volume | Chemically competent | Electrocompetent | Throughput | Advantage |
|---|---|---|---|---|
| One Shot 50 μL | ● | ● | Single-use | Transformation and recovery in the same tube |
| MultiShot StripWell 50 μL per well | ● | Medium-throughput | 12 strips of 8 tubes; do as many or as few reactions as needed | |
| MultiShot FlexPlate 20 μL per well | ● | Medium-to high-throughput | 96-well segmented plate: Use multiples of 8-well segments or entire plate | |
| MultiShot 20 μL per well | ● | High-throughput | 96-well full-skirted plates suitable for automation platforms | |
| Standard 100 µL, 200 µL, or 500 µL | ● | ● | Multi-use for throughput | Economical option |
Single-use One Shot aliquots for exceptional convenience
The single-use One Shot kits prioritize convenience
The One Shot format provides 50 μL aliquots of chemically competent cells in single-use tubes. Transformation is performed directly in the tube, improving efficiency at the bench. There are no extra pipetting steps, no extra tubes, and no loss of efficiency due to repeated freeze-thaw cycles. These kits come with SOC medium and pUC19 positive control.
MultiShot StripWell format for medium-throughput
MultiShot StripWell kits are designed for medium-throughput cloning
The MultiShot StripWell format provides strips of tubes in a 96-tube rack format, each well containing 50 μL of chemically competent cells This allows you to use as many or as few reactions as you want, without wasting reagents. Each kit includes 12 strips of 8 tubes, SOC medium, and pUC19 positive control.
MultiShot FlexPlate format for high-throughput
MultiShot FlexPlate gives you the freedom to choose your workflow
The MultiShot FlexPlate format provides a segmented 96-well plate with 20 μL of chemically competent cells in each well. The plate can be separated into 8-well segments allowing you to choose manual or automated applications. Kits are supplied with SOC medium and pUC19 positive control.
MultiShot format for very high throughput
MultiShot kits include competent cells re-dispensed into 96-well plates
The MultiShot format provides 20 μL aliquots of chemically competent cells in each well of a 96-well full-skirted Thermo Scientific Armadillo plate. Kits are supplied with SOC medium and pUC19 positive control.
Standard kits are the economical choice
Standard
bulk format for an economical choice
Standard kits include competent cells in a bulk format. They are designed for scientists who perform more than one transformation at a time and for general use in a busy lab. Kits are available in 500 μL (5 × 100 μL), 1 mL (5 × 200 μL), and 2 mL (4 × 500 μL) quantities. These kits include SOC medium and pUC19 positive control.
Have it your way with a custom kit
Custom kits with any competent cell type can be packaged in any of the above formats to meet your particular needs. Custom-designed strains can also be made available. Contact your local account manager or send an email to customorders@thermofisher.com to learn more.
Resources
Cloning Support Center
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Competent cells resources
Have any questions on competent cells or transformation? Click below to access overviews, videos, genotype guides and educational resources.
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For Research Use Only. Not for use in diagnostic procedures.
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