Each project has its own unique requirements. To meet these different demands, many of Invitrogen’s competent cells are offered in a variety of packaging formats. Custom formats are also available.

Competent cells are available in a variety of formats

Format / Volume Chemically competent Electrocompetent Throughput Advantage
One Shot 50 μl Single-use Transformation and recovery in the same tube
MultiShot StripWell
50 μl per well
  Medium-throughput

12 strips of 8 tubes; do as many or as few reactions as needed
MultiShot FlexPlate 20 μL per well   High-throughput
96-well segmented plate: Use multiples of 8-well segments or entire plate
MultiShot
15 μl per well
  High-throughput 96-well plates fit automated format
Standard
100 µl, 200 µl, or 500 µl
Multi-use for throughput Economical option

Single-use One Shot aliquots for unparalleled convenience

The single-use One Shot Kits offer the highest level of convenience

The single-use One Shot kits offer the highest level of convenience

The unique format provides 50 μl aliquots of chemically competent cells in single-use tubes. Transformation is performed directly in the tube, saving you time and effort. There are no extra pipetting steps, no extra tubes, and no loss of efficiency due to repeated freeze-thaw cycles. These kits come with SOC medium and pUC19 positive control.

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MultiShot StripWell format for medium-throughput

MultiShot StripWell kits are designed for medium-throughput cloning

MultiShot StripWell kits are designed for medium-throughput cloning

The MultiShot StripWell format provides strips of tubes in a 96-tube rack format, each well containing 50 μl of chemically competent cells This allows you to use as many or as few reactions as you want, without wasting reagents. Each kit includes 12 strips of 8 tubes, SOC medium, and pUC19 positive control.

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MultiShot FlexPlate format for high-throughput

MultiShot FlexPlate gives you the freedom to choose your workflow

MultiShot FlexPlate gives you the freedom to choose your workflow

The MultiShot FlexPlate format provides a segmented 96-well plate with 20 μL of chemically competent cells in each well. The plate can be separated into 8-well segments allowing you to choose manual or automated applications. Kits are supplied with 1 96-well plate, SOC medium, and pUC19 positive control.

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MultiShot format for the highest throughput

MultiShot kits include competent cells re-dispensed into 96-well plates

MultiShot kits include competent cells re-dispensed into 96-well plates

The MultiShot format provides 15 μl aliquots of chemically competent cells in each well of a 96-well plate. Kits are supplied with five 96-well plates, SOC medium, and pUC19 positive control.

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Standard kits are the economical choice

Standard kits include competent cells in a bulk format

Standard bulk format for an economical choice

Standard kits include competent cells in a bulk format. They are designed for scientists who perform more than one transformation at a time and for general use in a busy lab. Kits are available in 500 μl (5 × 100 μl), 1 ml (5 × 200 μl), and 2 ml (4 × 500 μl) quantities. These kits include SOC medium and pUC19 positive control.

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Have it your way with a custom kit

Custom kits with any competent cell type can be packaged in any of the above formats to meet your particular needs. Custom-designed strains can also be made available. Contact your local account manager or send an email to customorders@thermofisher.com to learn more.

Resources

Cloning Support Center

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Competent cells resources

Have any questions on competent cells or transformation? Click below to access overviews, videos, genotype guides and educational resources.

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